Detection of Precipitating and Complement Consuming Antibodies by Enzyme Linked Immune Sorbent Assay in Pigeon Breeders' Disease

Huizinga, M.; Lübbers, Meike; Berrens, L.

doi:10.1016/s0171-2985(84)80035-2

Cited by 5 publications

(7 citation statements)

References 5 publications

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“…The main aim of this study was to gather informa tion on the physico-and immunochemical properties of the PBC that are involved in ELISA-IgG antibody measurements against A. fumigatus antigen, and to elucidate the observed discrepancies between ELISAIgG titers and the corresponding numbers of precipi tates found in DI D [4,5,7,8].…”

Section: Discussionmentioning

confidence: 99%

“…Two different forms of discrepant observations were described: (1) high ELISA-IgG titers in the absence of detectable (or small) numbers of precipitates in DID [4,5,7,8,10], and (2) detectable precipitates with negative ELISA titers [4,7,19].…”

Section: Discussionmentioning

confidence: 99%

“…Next to the particular nature of the PBC involved in ELISA also the particular nature of the antibodies taking part in precipitation reactions might play a role [8,9]. It is known that for effective formation of precipitates interactions of the Fc part of immuno globulins is necessary [21].…”

Section: Discussionmentioning

confidence: 99%

“…However, discrepancies between the results of DID and ELISAIgG titers [3][4][5]7] have been reported. Different hypotheses were suggested for explain ing the observed discrepancies: (1) preferential bind ing of specific antigenic components to the polysty rene surface [4,7] which do not form precipitating complexes; (2) formation of immune complexes by certain antibody subclasses [5,8,9] of IgG that are not able to form precipitating immune complexes. In a previous publication [10] we have shown that polysty rene-binding antigenic components (PBC) are rapidly liberated during the logarithmic phase of growth of A .…”

Section: Introductionmentioning

confidence: 99%

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Antibody Determination against <i>Aspergillus fumigatus</i> by Means of the Enzyme-Linked Immunosorbent Assay

Kauffman¹,

Vanderheyden²,

Vanderlaan³

et al. 1985

Int Arch Allergy Immunol

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Polystyrene-binding antigenic components (PBC) of Aspergillus fumigatus involved in ELISA IgG antibody determinations and the corresponding numbers of precipitates found by double immunodiffusion (DID) and/or crossed immunoelectrophoresis (CIE) were studied using culture filtrate antigenic preparations fractionated by Sephadex G-100 chromatography, concanavalin A (ConA) affinity chromatography, isoelectric focusing (IEF) and TCA precipitation. It is shown that PBC, immunologically active in ELISA, are characterized by high molecular weights and ConA-binding properties. The pi values of these components were 3.5–6 and above 8. The glycoprotein and/or mucocpolysaccharide nature is suggested by the binding to ConA and their partial resistance to trichloroacetic acid treatment. By contrast, low-molecular weight components and antigenic components that are not absorbed by ConA being active in DID and/or CIE showed less or absence of binding to the polystyrene surface.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antibody Determination against <i>Aspergillus fumigatus</i> by Means of the Enzyme-Linked Immunosorbent Assay

Kauffman¹,

Vanderheyden²,

Vanderlaan³

et al. 1985

Int Arch Allergy Immunol

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“…These techniques exclude non-viable, morpho logically unidentifiable allergens, e.g., plant parts other than pollen [2,3], fragments of fungal hyphae and especially animal-derived proteins. The latter comprise components of serum, saliva, faeces, urine, pelts and feathers [4][5][6][7], Interest in occupational sensitization to animal al lergens among workers in laboratory animal facilities has increased in recent years [8][9][10]. Consequently, methods have been developed for estimating the level of exposure.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

Virtanen¹,

Louhelainen

Mäntyjärvi³

1986

Int Arch Allergy Immunol

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A method based on inhibition of the enzyme-linked immunosorbent assay was developed for measuring airborne bovine epidermal antigen (BEA) in cowsheds. BEA extract served as the solid-phase reactant competing with the sample for binding of a rabbit immune serum. A sample of swine epithelial extract was used to ascertain the specificity of the reaction. Cowshed air samples were collected according to a standardized volumetric filter method. A specific inhibiting activity was detected in all samples tested corresponding to concentrations between 137ng/m³ and 19.8 μg/m³. The intertest coefficient of variation of cowshed samples was 9.5%.

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IgM antiavian antibodies in sera from patients with pigeon breeder's disease

Martínez-Cordero¹,

De²,

2000

J. Clin. Lab. Anal.

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The authors' objective was to study the presence of IgM antiavian antibodies in sera from patients with pigeon breeder’s disease. We studied 93 patients with interstitial lung disease admitted for the assessment of pigeon breeder's disease. Eighty sera from healthy donors with no history of bird contact and 47 asymptomatic pigeon breeders were included as controls. The presence of IgM, IgG, and IgA antiavian antibodies was detected by ELISA and Western blot using avian‐pooled serum antigen. Fifty‐three patients were classified as having definite pigeon breeder's disease, whereas 40 did not fulfill these diagnostic criteria. The levels of IgM antiavian‐antibodies in pigeon breeder’s disease by ELISA exceeded both the values of healthy subjects with no history of avian contact (P = 2.5 × 10–8) and the results of asymptomatic breeders (P = 0.03). Positive IgA antiavian antibodies were the most frequent abnormalities in pigeon breeder's disease showing values over the reference levels of control groups that reach significant statistical differences. Both precipitin‐positive and ‐negative samples demonstrated IgM reactivity. IgM antiavian antibodies were confirmed by Western blot. A relationship of IgM positive tests with a recent history of avian antigen exposure and acute disease was found. Additionally, the positive IgM group included patients having subacute and chronic lung disease. Antiavian antibodies have previously been considered of minor significance in hypersensitivity pneumonitis; nevertheless, recent studies support their use in clinical diagnosis. Although no specific laboratory tests can confirm the diagnosis in pigeon breeder’s disease, IgM antiavian antibodies may be useful for detecting recent antigen exposure and the acute stage of the disease. J. Clin. Lab. Anal. 14:201–207, 2000. © 2000 Wiley‐Liss, Inc.

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Detection of Precipitating and Complement Consuming Antibodies by Enzyme Linked Immune Sorbent Assay in Pigeon Breeders' Disease

Cited by 5 publications

References 5 publications

Antibody Determination against <i>Aspergillus fumigatus</i> by Means of the Enzyme-Linked Immunosorbent Assay

Antibody Determination against <i>Aspergillus fumigatus</i> by Means of the Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

IgM antiavian antibodies in sera from patients with pigeon breeder's disease

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