Detection of Pityrosporum orbiculare reactive T cells from skin and blood in atopic dermatitis and characterization of their cytokine profiles

Linder, Maria Tengvall; Johansson, Catharina; Zargari, Arezou; Bengtsson, Å.; Ploeg, Ingeborg van der; Jones, Ilona; Härfast, B.; Scheynius, Annika

doi:10.1046/j.1365-2222.1996.d01-281.x

Cited by 63 publications

(43 citation statements)

References 27 publications

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“…Allergen specific T cells cloned from acute AEDS lesional skin show a Th2/Th0-like cytokine profile [64, 72], while the in situ expression of IL-4 in chronic AEDS lesions is accompanied by the expression of interferon-γ [73, 74], also suggesting an involvement of Th1 cytokines in the chronic phase. A possible scenario would be an influence by Th2 cytokines from allergen-specific T cells in the acute phase of AEDS.…”

Section: Possible Pathogenic Mechanismsmentioning

confidence: 99%

“…Elevated proliferation of PBMC in response to in vitro stimulation with Malassezia extract has been found in AEDS patients with specific serum IgE to Malassezia [16, 64, 65, 66]in combination with a Th2-like cytokine profile [65, 66, 67]. The Th2-like cytokines IL-4 and IL-13 support IgE production, while IL-5 promotes growth and differentiation of eosinophils [68], one of the early skin-infiltrating cells in the APT reaction [21, 69].…”

Section: Possible Pathogenic Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

Atopic Eczema/Dermatitis Syndrome and <i>Malassezia</i>

Scheynius

Johansson

Buentke

et al. 2002

Int Arch Allergy Immunol

118

133

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Atopic dermatitis is a chronic multifactorial inflammatory skin disease, which has had a marked increase in prevalence during the last decades. Recently, a new nomenclature was recommended where the term ‘atopic eczema/dermatitis syndrome’ (AEDS) should be used to reflect the heterogeneity in this group of patients and where those patients without measurable IgE reactivity should be classified as either ‘nonallergic AEDS’ or ‘non-IgE-associated allergic AEDS’. For nearly 20 years it has been discussed whether the opportunistic yeast Malassezia, previously designated Pityrosporum, is a contributing factor to AEDS. Today there are several reports that demonstrate specific serum IgE or positive skin prick test and/or atopy patch test reactions to Malassezia in patients with AEDS. Several IgE-binding components have been identified in extracts of Malassezia ranging in molecular mass between 10 and 100 kD. The genes for nine Malassezia allergens with molecular weights ranging from 14 to 36 kD have hitherto been identified and cloned. Six of them are now produced by recombinant techniques and used in diagnostic tests. At present the genus Malassezia is subdivided into seven different species, which all have been isolated from human skin. The respective contribution of different Malassezia spp. to AEDS and in what proportion they share allergens remains to be clarified. We summarize here data that Malassezia can play a role in eliciting and maintaining eczema in patients with AEDS.

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Section: Possible Pathogenic Mechanismsmentioning

confidence: 99%

Section: Possible Pathogenic Mechanismsmentioning

confidence: 99%

Atopic Eczema/Dermatitis Syndrome and <i>Malassezia</i>

Scheynius

Johansson

Buentke

et al. 2002

Int Arch Allergy Immunol

118

133

View full text Add to dashboard Cite

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“…Recently, it has been shown that both the acute and chronic AD lesions are associated with an increased expression of IL-13 mRNA when AD skin biopsy specimens were compared with those from normal control subjects using in situ hybridization [12]. Furthermore, it has been shown that IL-13 mRNA is detected in PBMC from patients with AD [13]. …”

Section: Introductionmentioning

confidence: 99%

IL-13 Production by Peripheral Blood Mononuclear Cells from Patients with Atopic Dermatitis

Takamatsu¹,

Hasegawa²,

Sato³

et al. 1998

Dermatology

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Background: Interleukin 13 (IL-13) shares several functional properties with IL-4 and has been shown to be capable of inducing IgE synthesis. Objective: To determine whether the production of IL-13 by peripheral blood mononuclear cells (PBMC) from patients with atopic dermatitis (AD) is connected with the development of AD. Methods: We examined PBMC from 52 patients with AD and 23 healthy volunteers as controls. PBMC were cultured with or without antigen extracted either from Dermatophagoides farinae or house dust for 48 h, and then the supernatants were measured for IL-13 by competitive enzyme immuno-assay. We also examined the effect of the immunosuppressant FK506 on the production of IL-13 by antigen-stimulated PBMC from AD patients. Results: The levels of IL-13 produced by non-stimulated PBMC in AD were significantly higher than those in controls. The IL-13 levels in AD were increased after antigen stimulation and were higher than those in controls. Moreover, the enhanced production of IL-13 in AD was suppressed by FK506. Conclusion: We suggest that IL-13 may play some role in the development of AD.

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“…Many reports clearly indicate the role of Malassezia in AD, especially AD located to the head and neck area in adults (19,26,29,30,41,62,66,69,74,83,98,109,125,131,132,135,136,137,148,149,154). The Malassezia yeasts have been cultured from patients with AD with the same frequency as from healthy individuals (19,41,52).…”

Section: Atopic Dermatitis and The Malassezia Yeastsmentioning

confidence: 99%

“…Several IgE binding components of Malassezia have been isolated (29,30,62,66,69,70,98,131,132,137,149,157,166,167). Three major allergen components from Malassezia were later identified using monoclonal antibodies (166).…”

Section: Atopic Dermatitis and The Malassezia Yeastsmentioning

confidence: 99%