Detection of pigeonpea sterility mosaic virus, the causal agent of sterility mosaic disease of pigeonpea in viruliferous mite vector by DAS-ELISA and DIBA

Latha, T. K. S.; Doraiswamy, Sabitha

doi:10.1080/03235400600940855

Cited by 10 publications

(10 citation statements)

References 3 publications

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“…Polyclonal antibodies to PPSMV particle preparations are effective in detecting PPSMV in plant tissues by the double antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) using enzyme‐labelled (alkaline phosphatase) immunogammaglobulin (Kumar et al ., , ). Serological diagnostic methods, such as DAS‐ELISA and dot‐immunobinding assay (DIBA), have been developed to detect the presence of PPSMV from the viruliferous vector using whole mite extract as an antigen (Latha and Doraiswamy, ). Both DAS‐ELISA and DIBA are sensitive for virus detection in vector mites; however, detection is possible only in groups of 10 or more mites.…”

Section: Virus Diagnosticsmentioning

confidence: 97%

“…in tomato and aubergine, A. mangiferae (Sayed) in mango, and Eriophyes cernuus (Massee) in ber (Singh and Raghuraman, ). Several A. cajani populations were collected from various SMD endemic locations in India, Nepal and Myanmar, and were subjected to PCR‐restriction fragment length polymorphism (RFLP) and sequencing for the ITS regions of rDNA (Kumar et al ., ; Latha and Doraiswamy, ). No significant variation was found in either the ITS regions of rDNA or the morphological features of the eriophyid mites.…”

Section: Virus Transmissionmentioning

confidence: 97%

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Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

Patil

Kumar

2015

Molecular Plant Pathology

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The disease is mainly controlled using SMD-resistant cultivars. However, the occurrence of distinct strains/isolates of PPSMV in different locations makes it difficult to incorporate broad-spectrum resistance. Studies on the inheritance of SMD resistance in different cultivars against different isolates of PPSMV indicate that the resistance is mostly governed by recessive genes, although there are contrasting interpretations of the data. Genetic engineering through RNA-interference (RNAi) and resistant gene-based strategies are some of the potential approaches for the transgenic control of SMD. Seed treatment or soil and foliar application of a number of organophosphorus-based insecticides or acaricides, which are recommended for the management of the vector mites, are seldom practised because of prohibitive costs and also their risks to human health and the environment.

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Section: Virus Diagnosticsmentioning

confidence: 97%

Section: Virus Transmissionmentioning

confidence: 97%

Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

Patil

Kumar

2015

Molecular Plant Pathology

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“…Spreading of virus-infected seeds in virus-free areas infested by A. tosichella could easily start a new infection by means of the mite. In contrast, PPSMV is transmitted only by A. cajani on pigeon pea and not by plant sap, seed or dodder (Latha and Doraiswamy, 2008; Maurya et al, 2017) sustaining the management of the virus disease by means of resistant varieties (Pallavi and Ramappa, 2014). The effectiveness of A. tosichella in transmitting viruses to the wheat can be influenced by the mite population composition and origin, and it might be related to the genetic mite lineage (Schiffer et al, 2009; Navia et al, 2013b; McMechan et al, 2014; Skoracka et al, 2018).…”

Section: Eriophyoids As Vectors Of Plant Virusesmentioning

confidence: 99%

“…The effects of the virus infected plants on the biology of the Eriophyidae are poorly known even though a strict co-evolution of the pathosystem may have produced advantages to mites. Aceria tosichella and A. cajani increase their fecundity rate and density, respectively, on WSMV and PPSMV infected and susceptible plant genotypes (Reddy and Nene, 1980; Kulkarni et al, 2002; Jones et al, 2004; Siriwetwiwat, 2006; Latha and Doraiswamy, 2008; Murugan et al, 2011; Skoracka et al, 2018). Field populations of P. fructiphilus were up to 17 times denser on rose rosette disease-symptomatic multiflora rose than on symptomless ones and the virus transmission was more efficient only when the mite was feeding on rapidly growing plant organs, which are more susceptible to the mite and more receptive for virus infection (Epstein and Hill, 1999).…”

Section: Eriophyoids As Vectors Of Plant Virusesmentioning

confidence: 99%

An Intimate Relationship Between Eriophyoid Mites and Their Host Plants – A Review

Lillo¹,

Pozzebon

Valenzano³

et al. 2018

Front. Plant Sci.

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Eriophyoid mites (Acari Eriophyoidea) are phytophagous arthropods forming intimate relationships with their host plants. These mites are associated with annual and perennial plants including ferns, and are highly specialized with a dominant monophagy. They can be classified in different ecological classes, i.e., vagrant, gall-making and refuge-seeking species. Many of them are major pests and some of them are vectors of plant pathogens. This paper critically reviews the knowledge on eriophyoids of agricultural importance with emphasis on sources for host plant resistance to these mites. The role of species belonging to the family Eriophyidae as vectors of plant viruses is discussed. Eriophyoid-host plant interactions, the susceptibility within selected crops and main host plant tolerance/resistance mechanisms are discussed. Fundamental concepts, subjects, and problems emerged in this review are pointed out and studies are suggested to clarify some controversial points.

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“…The puri ication procedure for minimizing the effects of components by extraction of infected leaves in buffers containing chelating and reducing agents, high concentrations of nonionic detergent, and the precipitation. Puri ication was achieved by quasi-equilibrium zoning centrifugation Sucrose and CsCl gradients [29]. In order to detect the presence of PPSMV from their virulent vector using whole mite extract as an antigen, serological diagnostic techniques such as DAS-ELISA (double antibody sandwich ELISA) and DIBA (dot immunobinding assay) were developed.…”

Section: Etiology and Virus Transmissionmentioning

confidence: 99%

Pigeonpea sterility mosaic virus a green plague-Current status of available drug and new potential targets

Singh¹,

Narula²,

Ujinwal³

et al. 2021

Ann Proteom Bioinform

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Pigeonpea is one of the important legume crops with high protein content and nutritional traits. It has enormous potency for its widespread adoption by farming communities. It is affected by various kinds of biotic and abiotic stresses. In the context, of biotic stresses Sterility mosaic disease (SMD) is one of the severe diseases in pigeonpea which ultimately lead to the drastic yield loss. The virus belongs to the genus Emaravirus, family- Fimoviridae. SMD is associated with two diverse types of Emaravirus, Pigeonpea sterility mosaic virus1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2). It is transmitted by the mite (Aceria cajani), mainly environmental contributing to the feasibility for the mites for the inoculation of the virus. The SMD is mainly governed by two genes SV1 that includes the dominant allele and serves as an inhibitory action on the resistance of the SV2. Methods for identification of the virus include RT-PCR, DIBA and ELISA using alkaline phosphatase or penicillinase. To control SMV disease farmers generally adopted intercropping methods. There are few potential drugs have been identified for the administration of the disease such as 0.1% Fenazaquin, Dicofol, Imidacloripid, Carbosulfan; Spiromesifin includes the inhibition of the mite inoculation on the pigeonpea plant. The present review describes compressive and systematic insights on SMV protein targets and potential drugs that could be utilized as the presumed drug targets for the finding of true drugs against the SMD in pigeonpea.

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Detection of pigeonpea sterility mosaic virus, the causal agent of sterility mosaic disease of pigeonpea in viruliferous mite vector by DAS-ELISA and DIBA

Cited by 10 publications

References 3 publications

Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

An Intimate Relationship Between Eriophyoid Mites and Their Host Plants – A Review

Pigeonpea sterility mosaic virus a green plague-Current status of available drug and new potential targets

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