Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS)

Sibat, Manoëlla; Herrenknecht, Christine; Darius, Hélène Taiana; Roué, Mélanie; Chinain, Mireille; Heß, Philipp

doi:10.1016/j.chroma.2018.08.008

Cited by 51 publications

(79 citation statements)

References 32 publications

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“…In HRMS spectra ( Figure 1A Figure 1B and Figure S1, Table S1). As with CTX3C and CTX1B [17], the opening of the G-and H-rings allowed the formation of the ion at m/z 563.3220 followed by water losses at m/z 545.3115, m/z 527.3009, and m/z 509.2903, these fragments were also detected in [20] ( Figure 1B and Figure S1, Table S1). Table S1).…”

Section: Lc-hrms Analysis In Targeted Ms/ms Mode Of C-ctx1mentioning

confidence: 77%

“…The complexity of the matrix and the difficulty in the ionization of CTXs also jeopardizes the applicability of LC-HRMS for the detection of CTXs. Despite this, the applicability of HRMS has been demonstrated in particular for CTX1B and CTX3C as the main toxins responsible of CP in the Pacific Ocean, the fragmentation pathways have been identified, increasing the knowledge on how these complex toxins fragment in the mass spectrometer [17][18][19]. Recent studies published by Kryuchkov et al [20] reported strategies for the structure elucidation of Caribbean ciguatoxin analogues (C-CTX3 and C-CTX4).…”

Section: Introductionmentioning

confidence: 99%

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Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts

Estevez

Sibat

Leão-Martins

et al. 2020

Toxins

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Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).Key Contribution: LC-Q-TOF-MS in full-scan MS and MS/MS mode permitted successful acquisition of C-CTX1 spectra. Fragmentation pathways are presented. C-CTX1 was confirmed in a barred hogfish (Bodianus scrofa) from the Selvagens Islands (Portugal).

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Section: Lc-hrms Analysis In Targeted Ms/ms Mode Of C-ctx1mentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts

Estevez

Sibat

Leão-Martins

et al. 2020

Toxins

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“…Several Gambierdiscus and Fukuyoa species/strains have been identified as likely producers of CTXs or CTX-like compounds, as measured by Mouse Biological Assay (MBA) [15,21,40,41], Receptor Binding Assay (RBA) [24], neuroblastoma cell-based assay (CBA-N2a) [42][43][44][45], fluorescent calcium flux assay [46], or liquid chromatography-tandem mass spectrometry (LC-MS/MS) [47][48][49][50][51]. However, the formal confirmation of the presence of previously characterized CTX-and/or MTX-group compounds using LC-MS/MS has been demonstrated only in a limited number of species, i.e., F. paulensis, G. australes, G. caribaeus, G. excentricus, G. pacificus, G. polynesiensis, and G. toxicus, although for this latter species, a potential misidentification of the species is likely [38].…”

Section: Introductionmentioning

confidence: 99%

“…Most of these toxigenic species/strains, however, exhibit very low toxicity, toxin production ranging from several femtograms to sub-picograms per cell [38,54]. Only two species proved significantly more toxic than the others, producing pg amounts of CTXs, namely G. polynesiensis [24,49,51] and G. excentricus [44,54,55], and are recognized as important toxin-producing species in the South Pacific and the Atlantic Oceans, respectively. As an example, published toxicity records for G. polynesiensis range from 1.2 up to 18.2 pg cell −1 , as determined by either RBA [24], CBA-N2a [47,56], or LC-MS/MS [49].…”

Section: Introductionmentioning

confidence: 99%

“…Based on their skeletal structures, CTX analogs can be separated into two types, i.e., CTX1B type and CTX3C type analogs [57]. Once accumulated in fish, algal CTXs are metabolized into more oxidized congeners [2,58,59], although the occurrence of small amounts of the oxidized forms of CTXs, i.e., 2,3-dihydroxy-P-CTX3C, 51-hydroxy-P-CTX3C, 2-hydroxy-P-CTX3C, M-seco-P-CTX3C has also been shown in cultures of G. toxicus and G. polynesiensis [26,51]. Complex biotransformation processes likely take place in fish livers [2] and lead to species-specific and region-specific toxin profiles in fish [26,57].…”

Section: Introductionmentioning

confidence: 99%

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Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia

Longo

Sibat

Viallon

et al. 2019

Toxins

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Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell−1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase.

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Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges

Panda

Dash

Manickam

et al. 2021

Mass Spectrometry Reviews

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There has been a rising concern regarding the harmful impact of biotoxins, source of origin, and the determination of the specific type of toxin. With numerous reports on their extensive spread, biotoxins pose a critical challenge to figure out their parent groups, metabolites, and concentration. In that aspect, liquid chromatography‐mass spectrometry (LC‐MS) based analysis paves the way for its accurate identification and quantification. The biotoxins are ideally categorized as phytotoxins, mycotoxins, shellfish‐toxins, ciguatoxins, cyanotoxins, and bacterial toxins such as tetrodotoxins. Considering the diverse nature of biotoxins, both low‐resolution mass spectrometry (LRMS) and high‐resolution mass spectrometry (HRMS) methods have been implemented for their detection. The sample preparation strategy for complex matrix usually includes “QuEChERS” extraction or solid‐phase extraction coupled with homogenization and centrifugation. For targeted analysis of biotoxins, the LRMS consisting of a tandem mass spectrometer operating in multiple reaction monitoring mode has been widely implemented. With the help of the reference standard, most of the toxins were accurately quantified. At the same time, the suspect screening and nontarget screening approach are facilitated by the HRMS platforms during the absence of reference standards. Significant progress has also been made in sampling device employment, utilizing novel sample preparation strategies, synthesizing toxin standards, employing hybrid MS platforms, and the associated data interpretation. This critical review attempts to elucidate the progress in LC‐MS based analysis in the determination of biotoxins while pointing out major challenges and suggestions for future development.

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Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS)

Cited by 51 publications

References 32 publications

Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts

Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts

Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia

Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges

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