Detection of myostatin gene MSTN in some goat breeds (Capra hircus)

Abstract

Till now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with the accession numbers KY463684 for Zaraibi and KY463685 for Baladi and Chinese goat breeds of the MSTN gene deposited with international gene banks by up to 99% and some other species including sheep, cows and bull breeds with percentages of 95 to 97% and between 95 to 99%, respectively. There is also a correlation between the sequences of the registered pieces of Baladi with KY463686 and Damascus and Chinese breeds with KY441464 of MSTN deposited with international gene banks by up to 99% and some other species including sheep and bull breeds at a ratio of 99% for two pieces. Results demonstrated the deposited sequences of object are part of intron 1, exon 2 is fully sequenced with Zaraibi and Baladi breeds; the intron 1, exon 1 with Baladi breed; and the intron 2, part of exon 3 with Damascus breed. Therefore, the Egyptian goat breeds consider national wealth can be used to develop breeding and improvement programs which helps in more applicable scopes like biotechnology, genetic engineering and molecular biology with the help of bioinformatics tools.

Utilization of Biotechnology, Neurotransmitter and Cytogenetic Indices in Selecting Pigeon Breeds

“…From the previous results, testing the racing pigeon breeds for both LDHA the DRD4 genes is important process in selecting new pigeon breeders and breeding perfect champion racing pigeons with high quality racing performance, capable of long flights and rapid return from distant released places through carrying possible variations of these genes and exclude the other pigeons not carry at least one of the these racing performance genes from breeding program. With the Baladi breed, intron 1, exon 1; and with the Damascus breed, intron 2, part of exon 3 with the bioinformatics tools, Egyptian goat breeds consider national wealth can be used to build breeding and improvement programmes that help in more applicable scopes such as biotechnology, genetic engineering, and molecular biology (44). Another indices studied in the current study and may be important in selecting racing pigeon breeds is cytogenetic indices including; chromosome structure and the precise identification of chromosomes using differential staining techniques such as G-banding and establishing a standard chromosome nomenclature for each breed.…”

Genetic variation of myostatin MSTN gene and protein polymorphism in three goat populations

“…The deposited sequences of objects are part of intron 3, and exon 3 is fully sequenced with three breeds, according to the findings. Part of intron 1, exon 2 is fully sequenced with Zaraibi and Baladi breeds; intron 1, exon 1 with Baladi breed; and intron 2, part of exon 3 with Damascus breed are among the deposited sequences of the item according to Dowidar et al (2018).…”

Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech

“…MSTN , the gene encoding myostatin, can generate more extensive muscling in cattle, sheep, and pigs, whereas the POLLED allele in cattle is commonly targeted for editing along with protein-encoding genes such as RELA. 110, 111, 112, 113, 114, 115 Editing of the CD163 gene for resistance to the porcine reproductive and respiratory syndrome (PRRS) in pigs is a recent achievement and has been used extensively in the production of PRRS-resistant pigs 116, 117…”