Till now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with the accession numbers KY463684 for Zaraibi and KY463685 for Baladi and Chinese goat breeds of the MSTN gene deposited with international gene banks by up to 99% and some other species including sheep, cows and bull breeds with percentages of 95 to 97% and between 95 to 99%, respectively. There is also a correlation between the sequences of the registered pieces of Baladi with KY463686 and Damascus and Chinese breeds with KY441464 of MSTN deposited with international gene banks by up to 99% and some other species including sheep and bull breeds at a ratio of 99% for two pieces. Results demonstrated the deposited sequences of object are part of intron 1, exon 2 is fully sequenced with Zaraibi and Baladi breeds; the intron 1, exon 1 with Baladi breed; and the intron 2, part of exon 3 with Damascus breed. Therefore, the Egyptian goat breeds consider national wealth can be used to develop breeding and improvement programs which helps in more applicable scopes like biotechnology, genetic engineering and molecular biology with the help of bioinformatics tools.

Semen was collected, evaluated and extended with two extenders (Lactose-yolk-citrate: LYC and Tris-yolk-fructose: TYF .The final extension rate was 1ml semen:4ml extender. The extended semen was divided into cervical 4 tubes added with Glutathion (GSH) at concentrations of 0.0, 0.2, 0.4 and 0.8 mM/100ml. The extended semen was kept at 5 ºC for5 days. After each storage time (0, 1, 2,3, 4 and 5 days), the percentages of sperm motility, dead spermatozoa, abnormal spermatozoa, and acrosome damage of spermatozoa were recorded. Activities of aspartate-aminotransferase (AST) and alanine-aminotrans ferase (ALT) enzymes were recorded. The penetrating ability of camel spermatozoa added with 0.4 mM into she-camel cervical mucus, during incubation at 37ºC for up to 4 hours was also recorded. The results showed that, the extended cooled camel semen with TYF extender was insignificantly higher the percentage of motile spermatozoa, while insignificantly lower the percentages of dead spermatozoa, abnormal spermatozoa and acrosome damage and leakage of AST and ALT enzymes into the extra cellular medium than LYC extender, during storage at 5ºC for 5 days with the different concentrations of glutathione (GSH) or free-GSH medium. The advancement of storage time at 5ºC for up to 5days was significantly (P<0.05) decreased the percentage of sperm motility , while increased significantly ((P<0.05) the percentages of dead spermatozoa, abnormal spermatozoa, acrosome damage and enzymatic activities(AST and ALT) with different extenders or GSH concentrations. The penetrating ability of the extended camel spermatozoa with TYF or LYC extender added with 0.4 mM/100 ml GSH into she-camel cervical mucus was significantly (P<0.05) better than free-GSH medium (control), during incubation at 37 ºC for up to 4 hours. However, the advancement of incubation time at