2019
DOI: 10.1371/journal.pone.0219071
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Detection of Mycoplasma anatis, M. anseris, M. cloacale and Mycoplasma sp. 1220 in waterfowl using species-specific PCR assays

Abstract: Mycoplasma anatis , M . anseris , M . cloacale and M . sp. 1220 colonise geese and ducks, and could be associated with infections of avian respiratory and nervous systems, cause mild to severe inflammation of cloaca and genital tracts, and embryo lethality. Co-occurrence of these Mycoplasma species in waterfowl is frequently detected and the identification of thes… Show more

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Cited by 13 publications
(28 citation statements)
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“…Similar to the domestic goose, cloaca and phallus inflammation were observed in the diseased birds in China. M. anserisalpingitidis was detected and/or isolated from the cloaca swabs of the sampled clinically ill and healthy swan geese like in domestic goose in Europe [20,21].…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Similar to the domestic goose, cloaca and phallus inflammation were observed in the diseased birds in China. M. anserisalpingitidis was detected and/or isolated from the cloaca swabs of the sampled clinically ill and healthy swan geese like in domestic goose in Europe [20,21].…”
Section: Resultsmentioning
confidence: 99%
“…The QIAamp DNA Mini Kit (Qiagen Inc., Hilden, Germany) was used for DNA extraction according to the manufacturers' instructions from the cultures and from the FTA cards. Species-specific PCR systems targeting M. anserisalpingitidis, M. anatis, M. anseris, M. cloacale and Acholeplasma species were used for screening and identification [21,22]. The purity of the cultures was confirmed and the unknown species were identified by a universal Mycoplasma PCR system targeting the 16S/23S rRNA intergenic spacer region in Mycoplasmatales followed by sequencing on an ABI Prism 3100 automated DNA sequencer (Applied Biosystems, Foster City, CA), sequence analysis and BLAST search [23,24].…”
Section: Methodsmentioning
confidence: 99%
“…The genus Mycoplasma belongs to the family Mycoplasmataceae of the class Mollicutes , and includes more than 125 species, which are phenotypically and genetically diverse, and are broadly distributed in nature, colonizing and/or infecting man and other vertebrates [1, 2]. Waterfowl of the family Anatidae , which includes ducks and geese, are susceptible to colonization and infection with some Mycoplasma species and can develop inapparent, chronic or acute mycoplasmosis [2–21]. Mycoplasma anatis , Mycoplasma anseris , Mycoplasma cloacale , Mycoplasma gallisepticum , Mycoplasma imitans , Mycoplasma synoviae and Mycoplasma sp.…”
Section: Full-textmentioning
confidence: 99%
“…It commonly occurs alongside M. anseris, M. cloacale, and M. anatis and is present all over the world. It can be isolated from geese and occasionally from ducks [3]. This species can be transmitted both vertically and horizontally [4,5].…”
Section: Introductionmentioning
confidence: 99%
“…In recent years the investigation of these bacteria have become a research priority [9]. In 2019 a speciesspecific PCR system was designed [3] that led to improved laboratory diagnosis. No vaccine is available for this mycoplasma as of yet, but antibiotics are used routinely to treat the infection [10].…”
Section: Introductionmentioning
confidence: 99%