Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Volokhov, Dmitriy V.; Grózner, Dénes; Gyuranecz, Miklós; Ferguson‐Noel, Naola; Gao, Yamei; Bradbury, Janet M.; Whittaker, Paul; Chizhikov, Vladimir E.; Szathmáry, Susan; Stipkovits, L.

doi:10.1099/ijsem.0.004052

Cited by 27 publications

(24 citation statements)

References 62 publications

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“…3). Sequence similarity analysis of the rpoB gene [8,17] showed 96.95-97.85% identity between MYCAV785 and M. anserisalpingitidis strains available in GenBank, while 90.14-90.24% identity was detected between MYCAV785 and the publicly available M. anatis strains. GenBank sequence accession numbers of the 16S rRNA and rpoB gene fragments of MYCAV785 are MT241511 and MT241512, respectively (Supplement material).…”

Section: Resultsmentioning

confidence: 96%

“…Mycoplasma infection of geese suffering from salpingitis was first reported by Kosovac and Djurisic [4]. Since then beside Acholeplasma species, M. anseris, M. anatis, M. cloacale, unidentified Mycoplasma species and most frequently M. anserisalpingitidis were identified and associated with reproductive diseases in waterfowl [1,[5][6][7][8]. After the first laying period about 15 to 20% of the ganders harbour M. anserisalpingitidis in the phallus lymph, cloaca and/or trachea.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

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Background: Mycoplasma anserisalpingitidis causes significant economic losses in the domestic goose (Anser anser) industry in Europe. As 95% of the global goose production is in China where the primary species is the swan goose (Anser cygnoides), it is crucial to know whether the agent is present in this region of the world. Results: Purulent cloaca and purulent or necrotic phallus inflammation were observed in affected animals which represented 1-2% of a swan goose breeding flock (75,000 animals) near Guanghzou, China, in September 2019. From twelve sampled animals the cloaca swabs of five birds (three male, two female) were demonstrated to be M. anserisalpingitidis positive by PCR and the agent was successfully isolated from the samples of three female geese. Based on whole genome sequence analysis, the examined isolate showed high genetic similarity (84.67%) with the European isolates. The antibiotic susceptibility profiles of two swan goose isolates, determined by microbroth dilution method against 12 antibiotics and an antibiotic combination were also similar to the European domestic goose ones with tylvalosin and tiamulin being the most effective drugs. Conclusions: To the best of our knowledge this is the first description of M. anserisalpingitidis infection in swan goose, thus the study highlights the importance of mycoplasmosis in the goose industry on a global scale.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

et al. 2020

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“…Mycoplasma anserisalpingitidis is a common waterfowl pathogen present worldwide [2,23]. Despite the economic losses it can cause, there are still major gaps in our knowledge about this microorganism.…”

Section: Discussionmentioning

confidence: 99%

“…Mycoplasma anserisalpingitidis has only recently been fully characterized [20], and as of the writing of this study, no cgMLST schema exists for M. anserisalpingitidis. The aim of the present examination was the genetic characterisation of 81 M. anserisalpingitidis strains with a robust and reliable cgMLST schema created in this study.…”

Section: Introductionmentioning

confidence: 97%

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The core genome multi-locus sequence typing of Mycoplasma anserisalpingitidis

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Background: Mycoplasma anserisalpingitidis is a waterfowl pathogen that mainly infects geese, can cause significant economic losses and is present worldwide. With the advance of whole genome sequencing technologies, new methods are available for the researchers; one emerging methodology is the core genome Multi-Locus Sequence Typing (cgMLST). The core genome contains a high percentage of the coding DNA sequence (CDS) set of the studied strains. The cgMLST schemas are powerful genotyping tools allowing for the investigation of potential epidemics, and precise and reliable classification of the strains. Although whole genome sequences of M. anserisalpingitidis strains are available, to date, no cgMLST schema has been published for this species. Results: In this study, Illumina short reads of 81 M. anserisalpingitidis strains were used, including samples from Hungary, Poland, Sweden, and China. Draft genomes were assembled with the SPAdes software and analysed with the online available chewBBACA program. User made modifications in the program enabled analysis of mycoplasmas and provided similar results as the conventional SeqSphere+ software. The threshold of the presence of CDS in the strains was set to 93% due to the quality of the draft genomes, resulting in the most accurate and robust schema. Three hundred thirty-one CDSs constituted our cgMLST schema (representing 42,77% of the whole CDS set of M. anserisalpingitidis ATCC BAA-2147), and a Neighbor joining tree was created using the allelic profiles. The correlation was observed between the strains' cgMLST profile and geographical origin; however, strains from the same integration but different locations also showed close relationship. Strains isolated from different tissue samples of the same animal revealed highly similar cgMLST profiles. Conclusions: The Neighbor joining tree from the cgMLST schema closely resembled the real-life spatial and temporal relationships of the strains. The incongruences between background data and the cgMLST profile in the strains from the same integration can be because of the higher probability of contacts between the flocks. This schema can help with the epidemiological investigation and can be used as a basis for further studies.

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Mycoplasma

Balish,

Chopra‐Dewasthaly,

Pereyre

et al. 2024

Bergey's Manual of Systematics of Archaea and Bacteria

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My.co.plas'ma. Gr. masc. n. mykês , mushroom or other fungus; Gr. neut. n. plasma , anything formed or molded, image, figure; N.L. neut. n. Mycoplasma , fungus form. Bacillota_I / Bacilli_A / Mycoplasmatales / Mycoplasmataceae / Mycoplasma Bacteria in the genus Mycoplasma are small (300–800 nm in diameter) pleomorphic cells devoid of a cell wall. Culturable species usually form very small (<1 mm) umbonate colonies on agar. Their use of the codon UGA to encode tryptophan is a distinctive characteristic of all species examined to date. As a consequence of their small (usually 0.5–1.5 Mb) genomes they have limited intermediary metabolism and are nutritionally fastidious, requiring exogenous carbohydrates or arginine, fatty acids, cholesterol or other sterols, peptides, cofactors, and free nucleic acids for axenic growth. In nature, all species are obligate commensals or parasites with varying degrees of specificity for a wide range of vertebrate hosts. The type species Mycoplasma mycoides subsp. mycoides and Mycoplasma capricolum subsp. capripneumoniae are highly virulent animal pathogens subject to strict international regulations, but the genus is perhaps better known for Mycoplasma pneumoniae , the agent of primary atypical “walking” pneumonia in humans. The relative biological simplicity of mycoplasmas confers significant advantages for current proteomics, metabolomics, synthetic genomics, and systems biology research. For example, the recent chemical synthesis and transplantation of intact chromosomes demonstrated that it is possible to enliven a mycoplasma fully capable of autonomous replication with an artificially constructed genome. DNA G + C content (mol%) : 23–40. Type species : Mycoplasma mycoides Freundt 1955 AL (basonym: Asterococcus mycoides Borrel et al. 1910.

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Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Cited by 27 publications

References 62 publications

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

The core genome multi-locus sequence typing of Mycoplasma anserisalpingitidis

Mycoplasma

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