Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

Gyuranecz, Miklós; Mitter, Alexa; Kovács, Áron Botond; Grózner, Dénes; Kreizinger, Zsuzsa; Bali, Krisztina; Bányai, Krisztiàn; Morrow, Christopher J.

doi:10.1186/s12917-020-02393-5

Cited by 17 publications

(13 citation statements)

References 24 publications

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“… Volokhov et al (2020) interpreted sequence analyses and suggested that the rpoB gene of Mycoplasma anserisalpingitidis could vary by approximately 4% between strains. It is also notable that analysis of the partial rpoB gene showed high similarity of our sequences to M. anserisalpingitidis isolates from distant geographical regions such as Hungary and China ( Gyuranecz et al, 2020 ). The authors found a close relationship between the M. anserisalpingitidis MYCAV93 strain isolated from the inflamed phallus of a domestic goose in Hungary in 2011 and MYCAV785 strain isolated from a clinically healthy swan goose from China.…”

Section: Discussionsupporting

confidence: 53%

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Evidence of Mycoplasma spp. transmission by migratory wild geese

et al. 2022

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Mycoplasma infections have been found in different species of waterfowl worldwide. However, the question of how the pathogens have been transmitted and dispersed is still poorly understood. Samples collected from clinically healthy greater white-fronted geese ( Anser albifrons ) (N = 12), graylag geese ( Anser anser ) (N = 6), taiga bean geese ( Anser fabalis ) (N = 10), and barnacle geese ( Branta leucopsis ) (N = 1) were tested for Mycoplasma spp. All Mycoplasma -positive samples were specified by species-specific PCR for Mycoplasma anserisalpingitidis (formerly known as Mycoplasma sp. 1220), M. anseris, M. anatis , and M. cloacale . The presence of Mycoplasma spp. was confirmed in 22 of 29 sampled birds (75.9%). Mycoplasma anserisalpingitidis was the most frequently detected species (15 of 22, 68.2%). However, we did not detect any of the other Mycoplasma spp. typical for geese, among which are M. anatis, M. anseris , and M. cloacale . Phylogenetic analysis revealed that Polish sequences of M. anserisalpingitidis formed a distinct branch, along with 2 Hungarian isolates obtained from domestic geese. Eight of the samples identified as Mycoplasma spp.-positive were negative for the aforementioned Mycoplasma species. A phylogenetic tree constructed based on partial 16S rRNA gene analysis showed that Mycoplasma spp. sequences collected from Polish wild geese represent a distinct phylogenetic group with Mycoplasma sp. strain 2445 isolated from a domestic goose from Austria. The results of our study showed that wild geese could be a reservoir and vector of different species of the Mycoplasma genus that can cause significant economic losses in the domestic goose industry.

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Section: Discussionsupporting

confidence: 53%

“…in wild geese is scarce. However, Gyuranecz et al (2020) found that M. anserisalpingitidis isolated from Chinese swan geese ( Anser cygnoides ) showed high genetic similarity with the European isolates.…”

Section: Introductionmentioning

confidence: 93%

Evidence of Mycoplasma spp. transmission by migratory wild geese

et al. 2022

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“…In a previous report, duck salpingitis was associated with C. psittaci infection [1], E. coli and Tetratrichomonas sp. [2], M. anserisalpingitidis [5,36], and R. anatipestife [4]. Moreover, reproductive disorders in layers were associated with infections of E. coli [37], Staphylococci, Mannheimia haemolytica, Streptococcus bovis [7], G.anatis [9,10], G. anatis biovar haemolytica [38].…”

Section: Multi-agents Of Salpingitis During Production Peak Of Laying Hens and Breeder Ducksmentioning

confidence: 99%

“…No practical approach is available to control the affected flocks due to lack of knowledge of pathogenesis and virulence factors. In a previous report, duck salpingitis was associated with Chlamydia psittaci (C. psittaci) infection [1], Escherichia coli (E. coli) and trichomonad [2], and Riemerella anatipestifer (R. anatipestifer) [3], while Mycoplasma anserisalpingitidis (M. anserisalpingitidis) infection was associated with reproductive diseases in domestic geese [4,5]. Mortality and average condemnation varied from 1.5% in breeding Pekin ducks to 1.98-5% in other breeder ducks, depending on the isolates, mixed infection and virulence of the strains.…”

Section: Introductionmentioning

confidence: 99%

Co-Infection of Escherichia coli, Enterococcus faecalis and Chlamydia psittaci Contributes to Salpingitis of Laying Layers and Breeder Ducks

et al. 2021

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Salpingitis is manifested as hemorrhagic follicular inflammation exudations and peritonitis, leading to reduced egg production and high culling of breeder flocks. From 2018 to 2021, increasing salpingitis during egg peak is threatening the poultry industry post-artificial insemination, both in breeder layers and breeder ducks across China. In our study, Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis) and Chlamydia psittaci (C. psittaci) were isolated and identified from the diseased oviducts using biochemical tests and PCR. To identify and isolate pathogenicity, we inoculated the isolates into laying hens via an intravaginal route. Later, laying hens developed typical salpingitis after receiving the combination of the aforementioned three isolates (1 × 105 IFU/mL of C. psittaci and 1 × 106 CFU/mL of E. faecalis and E. coli, respectively), while less oviduct inflammation was observed in the layers inoculated with the above isolate alone. Furthermore, 56 breeder ducks were divided into seven groups, eight ducks per group. The birds received the combination of three isolates, synergic infection of E. coli and E. faecalis, and C. psittaci alone via vaginal tract, while the remaining ducks were inoculated with physiological saline as the control group. Egg production was monitored daily and lesions of oviducts and follicles were determined post-infection on day 6. Interestingly, typical salpingitis, degenerated follicles and yolk peritonitis were obviously found in the synergic infection of three isolates and the birds inoculated with C. psittaci alone developed hemorrhagic follicles and white exudates in oviducts, while birds with E. faecalis or E. coli alone did not develop typical salpingitis. Finally, higher E. coli loads were determined in the oviducts as compared to E. faecalis and C. psittaci infection. Taken together, the combination of E. coli and E. faecalis, and C. psittaci could induce typical salpingitis and yolk peritonitis both in laying hens and breeder ducks. Secondary infection of E. coli and E. faecalis via artificial insemination is urgently needed for investigation against salpingitis.

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“…In waterfowls such as geese and ducks, Mycoplasma anatis, Mycoplasma anseris, Mycoplasma cloacale and Mycoplasma anserisalpingitis (formerly called Mycoplasma sp. 1220) are common pathogenic Mycoplasma species (Stipkovits & Szathmary, 2012;Otlu, 2016;Grózner et al, 2019b;Gyuranecz et al, 2020). These Mycoplasma species, together with other infectious microorganisms and environmental factors, play an important role in the immune system of geese.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods

Celebi

Sevimli

Büyük

et al. 2022

Braz. J. Poult. Sci.

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This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from the Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey's Broth and Agar media. To identify Mycoplasma species a Growth Inhibition Test was used. The identification was continued with species-specific PCR and sequence analysis which provide amplification of the genes dnaX, pcrA, rpoB, and the sequence of the 16S rRNA gene, respectively. In addition, Mycoplasma gallisepticum and Mycoplasma synoviae from pneumonic lung samples were directly analyzed via Multiplex Real-time PCR. As a result, 51 Mycoplasma strains were isolated and 32 were identified as Mycoplasma anatis, 9 as Mycoplasma anseris, 5 as Mycoplasma cloacale and 3 as Mycoplasma anserisalpingitis. Two Mycoplasma isolates that could not be identified were grouped in the same branch as a result of 16S RNA sequencing and their nearest neighbour was found to be Mycoplasma sp. 2045 (GenBankNo.MK615061.1). M. gallisepticum DNA was detected in 3 pneumonic lung samples and M. gallisepticum/M. synoviae DNAs were found simultaneously in 1 sample. While some Mycoplasma species identified in this study consolidated their place as pneumonic agents, some increased their potential to become a pneumonic agent when compared with cases caused by well-recognized Mycoplasma strains. Two isolates were identified as -Mycoplasma spp. as their 16S rRNA gene sequence identity levels scored below the threshold of 98.7% for species demarcation and still need to be defined whether they are possible representatives of a novel Mycoplasma species.

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Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

Cited by 17 publications

References 24 publications

Evidence of Mycoplasma spp. transmission by migratory wild geese

Evidence of Mycoplasma spp. transmission by migratory wild geese

Co-Infection of Escherichia coli, Enterococcus faecalis and Chlamydia psittaci Contributes to Salpingitis of Laying Layers and Breeder Ducks

Isolation of Mycoplasma spp. from Geese with Pneumonia and Identification of Microbial Isolates via Molecular Methods

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