Detection of Macroprolactinemia and Molecular Characterization of Prolactin Isoforms in Blood Samples of Hyperprolactinemic Women

Chawla, Rajinder; Antonios, Tibebe; Berhanu, Esaiyas; Ayana, Gonfa

doi:10.2478/v10011-011-0038-y

Cited by 7 publications

(5 citation statements)

References 22 publications

(19 reference statements)

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“…The secretion of endometrial prolactin helps in maintaining endometrial receptivity, and has been shown to provide an optimal environment for the implanting blastocyst transferred during in-vitro fertilization (IVF) cycles. A high enough prolactin level can inhibit the proliferation of luteinizing granulosa cells, and can also interfere with corpus luteum function resulting in luteal phase defect, as well as abnormal implantation, and embryo development [ 2 ]. Different studies have proven the existence of a state of hyperprolactinemia during IVF/Intracytoplasmic sperm injection (ICSI) cycles [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in serum prolactin level during intracytoplasmic sperm injection, and effect on clinical pregnancy rate: a prospective observational study

Kamel

Halim

Shehata

et al. 2018

BMC Pregnancy Childbirth

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BackgroundTransient hyperprolactinemia was proven to adversely affect the outcome of IVF. We aimed to identify changes in serum prolactin levels in patients undergoing ICSI, and to evaluate the effect of these changes on the clinical pregnancy rate.MethodsA prospective observational study included 90 patients scheduled for ICSI cycles. In each case 4 serum samples were collected during the cycle (midluteal, before ovum pick up procedure (OPU), 2 h after OPU, and before embryo transfer). Serum prolactin level was determined by immunoassay each time.ResultsThe sample collected 2 h after OPU had a mean difference of 25.8 ± 2.8 ng/ml compared to the basal serum prolactin (p < 0.01). In comparison to other samples, this highlighted a significant hyperprolactinemia occurring after OPU, and resolving before embryo transfer. No statistically significant difference between the different serum prolactin samples amongst the pregnant and non pregnant patients. There was a significant positive pearson correlation between the prolactin levels before OPU, and the presence of higher quality embryos (r = 0.274, p = 0.019).ConclusionIn normoprolactinemic women transient hyperprolactinemia is identified in patients undergoing ICSI, and it doesn’t affect the clinical pregnancy rates. A positive correlation was identified between higher quality embryos, and serum prolactin level before OPU.Trial registrationClinicalTrials.gov Identifier: NCT02292953, First received: November 10, 2014.

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Section: Introductionmentioning

confidence: 99%

Changes in serum prolactin level during intracytoplasmic sperm injection, and effect on clinical pregnancy rate: a prospective observational study

Kamel

Halim

Shehata

et al. 2018

BMC Pregnancy Childbirth

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“…This formulation of PRL may cause limited bioactivity, bioavailability and clearance from the glomerulus. Macroprolactinemia is usually asymptomatic, patients with macroprolactinemia do not need further therapy, and pregnancy is possible without any treatment for such hyperprolactinemia [ 4 – 6 ]. Although the gold standard method for detecting macroPRL is gel filtration chromatography, precipitation with polyethylene glycol (PEG) is a widely used screening test.…”

Section: Introductionmentioning

confidence: 99%

Serum macroprolactin levels in pregnancy and association with thyroid autoimmunity

et al. 2015

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BacgroundTo assess the contribution of macroprolactin to high serum prolactin levels and their association with thyroid status and thyroid autoimmunity during pregnancy.Methods138 pregnant women who suspected of having thyroid dysfunction were studied and divided into three groups according to the thyroid status; group 1; euthyroidism (n 40), group 2; hypothyroidism (n 54), and group 3; hyperthyroid (n 44). Polyethylene glycol (PEG) precipitation method was used for detection of macroprolactin. A percentage recovery of 40 % or less is considered as macroprolactinemia. If macroprolactin was negative, the percentage of monomeric prolactin recovery (monoPRL %) after PEG precipitation was used for comparison between the groups.ResultsMacroprolactinemia was found in two patients (1.4 %) one from hypothyroid and other from euthyroid group. Basal prolactin levels in these patients were 400 and 403 ng/mL respectively. Referring to all patients, there was no correlation between PRL, macroPRL or monoPRL % with thyroid hormone status and also with the serum levels of thyroid antibodies (p > 0.05). A positive correlation was observed between the serum levels of PRL with TSH (p = 0.014 and r = 0.219), while a negative correlation was found with FT4 (p = 0.011 and r = −0.227).ConclusionsDespite the fact that serum prolactin levels were found to be high during pregnancy, the contribution of macroprolactin was found to be insignificant in our study. Unlike other auto immune diseases, we could not find any relationship between thyroid autoimmunity and PRL, macroPRL or monoPRL %. These results confirmed that measured prolactin was quite homogeneous during pregnancy.

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“…Prl plays an important role in controlling reproduction. Although it's level increase beyond normal (Hyperprolactinemia) can be either physiological, pathological, or idiopathic, its clinical manifestations can vary from extreme to none (Majumdar and Mangal, 2013;Chawla et al, 2012). Endometrial cells were found to have prolactin receptors.…”

Section: Introductionmentioning

confidence: 99%

Role Of Prolactin And B-Hcg In Female Infertility

Ambad

Butola

Dhok

et al. 2021

ijrps

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The World Health Organization (WHO) describes infertility as a disability for couples in childbirth to attain pregnancy within 12 months of daily sexual intercourse. The reproductive years of women starts when she starts her menstrual cycle during puberty and capacity to have a child typically ends about age (45) years. However, it may be possible for a woman to become pregnant before her cycles end with menopause. Infertility affects 13–20 per cent of couples worldwide, regardless of race or ethnicity. The present study aim was To study the levels of prolactin and beta hCG in female infertility. And To correlate the levels of prolactin and beta hCG in infertile womens and healthy controls. The present study was conducted in the departments of biochemistry and dept. of obstetrics and gynaecology. The study was conducted on two groups of 40 infertile Females and 40 healthy controls. Serum sample used for estimation of prolactin and hCG, assessed by dry chemistry analyzer. Serum prolactin levels in infertile females is 33.96±11.46 and hCG levels was 86.38±12.45, which was higher in infertile female as compared to healthy control with a p-value of<0.05. The present study concludes that Hyperprolactinemia is a major contributing hormonal factor in infertility among infertile women and, as such, prolactin and hCG should be measured in infertile women.

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Detection of Macroprolactinemia and Molecular Characterization of Prolactin Isoforms in Blood Samples of Hyperprolactinemic Women

Cited by 7 publications

References 22 publications

Changes in serum prolactin level during intracytoplasmic sperm injection, and effect on clinical pregnancy rate: a prospective observational study

Changes in serum prolactin level during intracytoplasmic sperm injection, and effect on clinical pregnancy rate: a prospective observational study

Serum macroprolactin levels in pregnancy and association with thyroid autoimmunity

Role Of Prolactin And B-Hcg In Female Infertility

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