Detection of ISAba1 in Acinetobacter baumannii Strains Carrying OXA Genes Isolated From Iranian Burns Patients

Abstract

Background: The presence of carbapenemase-producing Acinetobacter baumannii has become a growing concern in patients who are hospitalized in burns centers. Objectives: The aims of this study were to determine the antimicrobial susceptibility patterns and prevalence of blaOXA carbapenemases, as well as to detect the presence of ISAba1, in A. baumannii strains carrying OXA genes obtained from burns patients at Shahid Motahari hospital, Tehran, Iran. Methods: From August 2013 to March 2014, 100 clinical A. baumannii isolates were collected from patients who were admitted to the burns ward at Shahid Motahari hospital. Antimicrobial susceptibility was determined using a disc diffusion test. PCR, sequencing, and multiplex PCR were used for the detection of blaOXA-23-like, blaOXA-51-like, blaOXA-24-like, and blaOXA-58-like genes, which were then sequenced. The ISAba1 gene was detected, and PCR was performed to detect the presence of ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like genes. Results:The results showed that 93% of the strains were multi-drug resistant, while 82% of them were extensively drug resistant. Additionally, all the strains carried blaOXA-23-like and blaOXA-51-like genes, while 74% and 0% of the strains harbored blaOXA-24-like and blaOXA-58 genes, respectively. ISAba1 was detected in all the strains except for one. The co-existence of ISAba1/blaOXA-51-like genes and ISAba1/blaOXA-23-like genes was detected in 65% and 80% of strains, respectively. Conclusions:The results of this study indicate that the emergence of OXA-type carbapenemases in A. baumannii causing nosocomial infections in burns patients could be of importance for hospital infection control systems in Iran.

Molecular Characterization of Acinetobacter baumannii Isolated from Ventilator-Associated Pneumonia and Burn Wound Colonization by Random Amplified Polymorphic DNA Polymerase Chain Reaction and the Relationship between Antibiotic Susceptibility and Biofilm Production

“…[10] Another study from Iran by Mohammadi et al in 2016 confirmed that all isolated carbapenem-resistant strains of A. baumannii isolated from VAP had oxa-23 gene. [9] The results of these studies[191016] are similar to the recent research that confirmed the high prevalence of oxa-23 compared to other carbapenemase in carbapenem-resistant A. baumannii . The high rate of isolated XDR A. baumannii formVAP and burns is one of alarming result in this study.…”

“…Hence, infected patients with MDR and/or XDR strains of A. baumannii in VAP and burn patients can increase mortality and morbidity. [12389] The detection of MDR and/or XDR strains of A . baumannii is a crucial step for nosocomial infection committees to control infectious spread of such organism.…”

Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran

“…Moreover, Mohammadi et al reported similar results among hospitalized patients with burn infection in 2016 in Iran [32]. Therefore, it is not surprising that Chen et al in 2017 in China, Uwingabiye et al in 2017 in Morocco, and Nowak et al in 2017 in Greece, Italy, and Spain reported similar results in their investigations [33], [34], [35].…”

Cloning, expression, purification and functional analysis of a specific multi-epitope protein from multi drug resistance Acinetobacter baumannii

“…In addition, the produced the partial to complete protection against strain harboured bla OXA-51like , bla OXA-23like , and bla OXAbacteria in mice. 24-like genes (26). E. coli strain Top10 was used for the The sequencing of microbial genomes has been provided the potential antigens of each pathogen (24).…”