Molecular Characterization of Acinetobacter baumannii Isolated from Ventilator-Associated Pneumonia and Burn Wound Colonization by Random Amplified Polymorphic DNA Polymerase Chain Reaction and the Relationship between Antibiotic Susceptibility and Biofilm Production

Asadian, Mahla; Azimi, Leila; Alinejad, Faranak; Ostadi, Yalda; Lari, Abdolaziz Rastegar

doi:10.4103/abr.abr_256_18

Cited by 17 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of bla OXA23 in a substantial proportion of isolates indicates its importance as a carbapenemase-encoding gene in the studied population. We identified bla OXA48 in 39% of the isolates, which shows variation compared to [36] and contradicts the findings of [38,39], where none of the isolates carried bla OXA48 . This finding suggests the emergence of bla OXA48 in the studied population, emphasizing the need for ongoing surveillance and monitoring of resistance mechanisms.…”

Section: Discussioncontrasting

confidence: 99%

“…The second most prevalent gene identified in our study was bla OXA23 , detected in 74% of the isolates. This finding aligns with the studies conducted by [32,[36][37][38], but slightly differs from [37]. The presence of bla OXA23 in a substantial proportion of isolates indicates its importance as a carbapenemase-encoding gene in the studied population.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Jasim,

Hadi,

Alsherees

et al. 2023

Med.Sci.Jour.Adv.Res

View full text Add to dashboard Cite

Acinetobacter baumannii is a highly problematic bacterium due to its multidrug-resistant nature and ability to rapidly develop resistance. Carbapenem-resistant A. baumannii (CRAB) is particularly concerning, recognized as a top priority pathogen by the World Health Organization (WHO). In this study, we aimed to assess the susceptibility of A. baumannii to antibiotics and characterize the presence of specific carbapenem resistance genes using PCR analysis. The study was conducted as a cross-sectional investigation at Al-Sader Medical City and Baghdad Teaching Hospitals between October 2022 and February 2023. A total of 59 A.baumannii isolates were collected from patients. To ensure accuracy in diagnosis, the isolates underwent thorough processing using morphological techniques, biochemical tests, and Vitek2 systems. The susceptibility of the isolates to 24 antibiotics was evaluated using the Kirby-Bauer method. Additionally, DNA extraction and PCR analysis were performed to detect the presence of carbapenem resistance genes. The study included analysis of 59 specimens collected from patients, including sputum, wound swabs, blood, and inguinal swabs. The majority of the isolates were obtained from in-patients, demonstrating a significant difference compared to outpatients. Among the studied isolates, the most prevalent carbapenem resistance gene detected was blaOXA51, present in 97% of the isolates. This highlights the substantial presence of blaOXA-mediated carbapenem resistance among A. baumannii strains in our study population. Furthermore, our findings revealed alarmingly high resistance rates against the majority of antibiotics commonly used to treat A. baumannii infections. Approximately 95% of the isolates were classified as extensively drug-resistant, indicating resistance to multiple classes of antibiotics. This poses significant challenges in terms of effective treatment options and emphasizes the urgent need for alternative strategies to manage A. baumannii infections.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 90%

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Jasim,

Hadi,

Alsherees

et al. 2023

Med.Sci.Jour.Adv.Res

View full text Add to dashboard Cite

show abstract

“…In 2018, the molecular epidemiology of 32 ventilator-associated pneumonia cases and 47 burn wound cases showed the colonization of extensively drug-resistant isolates of A. baumannii in 2 teaching hospitals in Tehran, Iran, indicating genetic similarity among all the isolates. 65 Furthermore, the genome analysis of MDR A. baumannii has identified the presence of transposons, integrons, and other MGEs like AbaR ( A. baumannii antibiotic resistance) islands. In a separate study from South India, the phylogenetic relationship of 14 biofilm-producing A. baumannii strains was investigated using RAPD-PCR.…”

Section: Random Amplified Polymorphic Dna-prcmentioning

confidence: 99%

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Anwer

2024

SMJ

View full text Add to dashboard Cite

“…Samples collected from body sites other than burn wonds, and those lacking phenotypical resistance, were excluded from the study. All strains were subcultured on EMB agar and Blood agar, followed by an incubation period of 18…”

Section: Sampling and Isolation Of Acinetobacter Baumannii Specimensmentioning

confidence: 99%

Designing a Multiplex PCR for Rapid and Accurate Detection of Metallobetalactamases Resistant Genes from Acinetobacter baumaniiIsolates in Tehran City, Iran

Mottaghiyan,

Esmaeili,

Ahmadi

et al. 2023

Iran J Pathol

View full text Add to dashboard Cite

Scan to discover onlineBackground & Objective: Acinetobacter baumannii strains harboring Meallobetalactamases (MBL) pose a significant threat in the context of nosocomial infections. The present investigation was undertaken with the objective of devising a Multiplex PCR methodology for the concurrent detection of MBL genes within A. baumannii strains prevalent in Tehran City, Iran.Methods: Between October 2020 and February 2021, 100 strains of A. baumannii were procured from burn specimens of hospitalized patients at Motahhari Hospital in Tehran. The identification of A. baumannii strains involved conventional biochemical techniques, coupled with confirmation of the presence of the bla OXA-51 gene. Antibiotic susceptibility was assessed using the Kirby-Bauer disc diffusion test. MBL-producing strains were characterized through a phenotypic approach employing the combined disk test, alongside Multiplex PCR for the simultaneous identification of bla VIM, bla IMP, bla GIM, and bla NDM genes. Statistical analyses were conducted using the chi-square test, with SPSS version 20.0 employed for data processing.Results: Among 100 strains examined, 96.1% exhibited positivity for MBL, as determined by the combined disk test. The study revealed a predominance of extensively drug-resistant (XDR) strains, with colistin demonstrating the highest level of sensitivity. The genotypic assay unveiled that Multiplex PCR identified bla VIM, bla NDM, and bla IMP in 20 strains, bla VIM and bla NDM in 30 strains, and exclusively the bla NDM gene in 45 strains. Notably, the Multiplex PCR technique exhibited the capacity to concurrently detect MBL genes (bla VIM, bla IMP, bla GIM, bla NDM) in 2 strains. Conclusion:The current investigation underscores prevalence of the bla NDM gene within clinical strains of A. baumannii. Furthermore, Multiplex PCR emerges as a robust and highly sensitive technique for rapid discernment of the MBL genes within in A. baumannii strains.

show abstract

Molecular Characterization of Acinetobacter baumannii Isolated from Ventilator-Associated Pneumonia and Burn Wound Colonization by Random Amplified Polymorphic DNA Polymerase Chain Reaction and the Relationship between Antibiotic Susceptibility and Biofilm Production

Cited by 17 publications

References 12 publications

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Designing a Multiplex PCR for Rapid and Accurate Detection of Metallobetalactamases Resistant Genes from Acinetobacter baumaniiIsolates in Tehran City, Iran

Contact Info

Product

Resources

About