Detection of IgE Mediated Immediate Hypersensitivity Reactions in the Sera of Ragweed Sensitive Individuals Using Rat Mast Cells

A Modified Rat Mast Cell Technique for the Detection of Human IgE-Type Reagins

“…This finding has been found useful in the detection of reagins in the sera of penicillin-f 1-3], ragweed- [4] and shellfish-sensitive [5] individuals. The purpose of the present study was to modify this rat mast cell technique in order to make it suitable for the routine analysis of a large number of allergic sera or derivative fractions.…”

Inhibition of Rat Mast Cell Degranulation and Histamine Release by Histamine-Rat Gammaglobulin Conjugate

“…RMCD was observed un der the microscope. 100 mast cells were counted and the number of degranulated cells were esti mated [4,8], This experiment allowed the selection of a proper concentration (0.63 mg/kg) of the compound 48/80 to be used for further studies.…”

The Effect of Laundry Detergent Containing <i>B. subtilis</i> Enzyme in Producing Sensitization Reactions in Guinea Pigs

“…The same procedures for the preparation of the mast cell suspension and test with human allergic serum and antigen have been used in the present study and has been described previously [12,13]. Briefly, equal volumes of 0.05 ml of guinea pig serum, alcalase solution and mast cell sus pension were incubated at 37 °C for 3 min.…”

“…The purpose of the present study, was to simulate conditions of consumer use of enzymecontaining detergents in the guinea pigs in order to establish whether ex posure of these animals to an alcalase-containing detergent by inhala tion, topical and abraded skin application and oral ingestion routes would yield allergic manifestations. Examination of these animals on long-term exposure revealed sensitization of the jejunum and the pres ence of circulating antibodies to alcalase as determined by the rat mast cell technique [12][13][14].…”