DETECTION OF IgE(IgND) SPECIFIC ANTIBODY (PROBABLY REAGIN) TO CASTOR-BEAN ALLERGEN BY THE RED-CELL-LINKED ANTIGEN-ANTIGLOBULIN REACTION

Immunochemical and Biological Analysis of Allergenicity with Excretory-Secretory Products of <i>Anisakis simplex</i> Third Stage Larva

“…In spite of the intensive investigation using animal models, such as mice or rats, the verification of L3-specific allergen was not successfully established in an animal model. Any specific mouse IgE production was not immunochemically verified [20, 25]. Amano et al [10]researched L3-dependent allergy with rat sera administered by L3-peritoneal inoculation, using passive cutaneous anaphylaxis, an in vivo method, but by neither indirect ELISA nor immunoblot for IgE, an in vitro method.…”

“…Avidin-conjugated alkaline phosphatase, Sigma 104 of alkaline phosphatase substrate, and p -nitrophenyl N-acetyl-β- D -glucosaminide were purchased from Sigma (St. Louis, Mo., USA). RBL-2H3 obtained from NIH (John Rivera’s Laboratory, Bethesda, Md., USA) [20]was maintained in our laboratory. The cells were cultured with MEM-Earles (Sigma), supplemented with 15% fetal bovine serum, L -glutamine, sodium pyruvate, and penicillin/streptomycin.…”

“…RBL-2H3 cells, a rat mucosal mast cell line, release a cellular metabolite by both specific IgE and allergens [16]. As a kind of biological method, the induction of RBL-2H3 exocytosis was highly correlated with passive cutaneous anaphylaxis, which is the biological method frequently used when no demonstration of specific IgE or allergen is possible [10, 17, 18, 19, 20]. …”

Reaginic Antibodies on Basophils in Grass Pollen Allergy, as Shown by Rosette-Formation

“…The RCLAAR has been used previously for the detection of IgE and other classes of antibodies specific for allergens such as the castor bean [Coombs et al, 1968], insulin [Devey et al, 1970] and penicillin [Wheeler, 1971], and has shown good correlation between the presence of specific IgE and the occurrence of clinical symptoms of hypersensitivity. In the present work, however, the test proved to be of much lower sensitivity for the detec tion of reaginic antibodies than the basophil rosette-forming reaction, since only 6 of the 15 individuals giving basophil rosettes had detectable circulating IgE antibodies.…”

“…Sera were tested for anti-pollen antibodies by the red cell-linked anligen-antiglobulin reaction (RCL.AAR) which was performed essentially as described by Coombs et al (1968], Erythrocytes coated with mixed grass pollen allergens as de scribed above were washed twice with PBS and added as 2% suspension to equal volumes of serial dilutions of the serum to be tested. After 30 min at room tem perature.…”