Immunochemical and Biological Analysis of Allergenicity with Excretory-Secretory Products of &lt;i&gt;Anisakis simplex&lt;/i&gt; Third Stage Larva

Kim, J.S.; Kim, K.H.; Cho, S.; Park, H.Y.; Cho, S.W.; Kim, Y.T.; Joo, Kyoung-Hwan; Lee, J.S.

doi:10.1159/000084225

Cited by 20 publications

(23 citation statements)

References 44 publications

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“…These differences between the results of indirect ELISA and Western blotting resulted in the profiling of the protein compositions of each ESP, using Microcon YM-100 and YM-3 filters (Millipore, Billerica, Massachusetts, USA). The majority of L3-ESP consists of low molecular weight (MW) proteins or peptides, and the serum antibodies from the rat anisakiasis serum were not found to bind to any of the low MW proteins (Kim et al, 2005). L3-ESP harbors a low proportion of proteins in excess of YM-100 kDa, and a high proportion of those less than YM-3 kDa (for simplicity, the material recovered from the YM-100 filters was described as being in excess of YM-100 kDa, and the material filtered through YM-3 filters was described as less than YM-3 kDa).…”

Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

“…The non-fragmented protein from L3-ESP has been regarded as sufficiently large to be processed by antigen-presenting cells and presented to T cells, resulting in activation of T cells. This T cell activation would result in an increase in the production of several rat antibody isotypes specific for L3-ESP, including IgE (Kim et al, 2005).…”

Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

“…However, when biological allergenicity was assayed via activation of RBL-2H3 cells, L3-ESP was found to be comparable to L4-ESP. Biological allergenicity can be confirmed by observing binding of specific IgE to the allergen (Kim et al, 2005). These conflicting results indicate that IgE generated in a biological allergic state may differ from the IgE identified by immunochemical methods, at least in the case of L3-ESP.…”

Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

“…Both Kim et al (2005) and Cho et al (2006) utilized the oral L3 infection of rats to explore allergies to L3, and their findings have contributed to our understanding of allergies in humans. The identification of specific IgE is the initial step in the diagnosis of an allergic reaction.…”

Section: Specific Immunoglobulin E Production and Allergy To L3mentioning

confidence: 99%

“…Amano et al (1995) insisted that the allergic reaction was more severe in cases of re-infection than in primary infection, which is consistent with the generally accepted allergy model. After the peritoneal inoculation of rats with L3, however, the levels of PCA reaction were related inversely to the numbers of infecting L3.Both Kim et al (2005) and Cho et al (2006) utilized the oral L3 infection of rats to explore allergies to L3, and their findings have contributed to our understanding of allergies in humans. The identification of specific IgE is the initial step in the diagnosis of an allergic reaction.…”

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Immune reactions and allergy in experimental anisakiasis

Cho

Lee

2006

Korean J Parasitol

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Abstract:The third-stage larvae (L3) of the parasitic nematode, Anisakis simplex, have been implicated in the induction of hyperimmune allergic reactions in orally infected humans. In this work, we have conducted a review of an investigation into immune reactions occurring in animals experimentally infected with A. simplex L3. The patterns of serum antibody productions in the experimental animals against excretory-secretory products (ESP) of A. simplex L3 contributed to our current knowledge regarding specific humoral immune reactions in humans. In our review, we were able to determine that L3 infection of experimental animals may constitute a good model system for further exploration of immune mechanisms and allergy in anisakiasis of humans. worldwide (Mattiucci et al., 1997;Shih, 2004;Ugland et al., 2004). Many epidemics have been reported in Japan and Spain (Chai et al., 2005). Increasing reports of L3 infection in fish have been documented in South Korea, in which the consumption of raw marine fish is also popular (Chai et al., 1992;Im et al., 1995;Song et al., 1995Song et al., , 1999. Easy access to endoscopes and enhanced awareness of anisakiasis among clinicians has resulted in better reporting of morbidity resultant from L3 infection. L3 infection induces the production of specific antibodies and cytokines (Kennedy et al., 1988;Daschner et al., 2001;Nieuwenhuizen et al., 2006). Antibodies can be detected 2 weeks after infection, consistent with the time courses associated with other microorganisms. Analyses of specific antibody levels are generally irrelevant to the differential diagnosis of an acute state in cases of L3 infection, because the profound pain associated with L3 penetration begins only a few hours after the consumption of infected raw fish. However, antibody level measurements are helpful both in the differentiation of tumors from the granulomas formed by infiltrating L3 and in investigations of allergic diseases (Gutierrez and Cuellar, 2002;Kim et al., 2006).The production of IgE tends to increase during parasite infections, but the ultimate effects of IgE vary considerably, depending on the host-parasite relationships. Hyperimmune allergic reactions have been closely associated with IgE production. The infection of a parasite into its normal host tends to reduce the development of allergic responses, despite the associated upshift in IgE production (van den Biggelaar et al., 2000;Yazdanbakhsh et al., 2002). By contrast, the infection of T. canis in humans, an abnormal host, induces increased allergic reactions (Sharp and Olson, 1962;Sharghi et al., 2001). A. simplex L3 has also been shown to induce allergic diseases at a high rate, principally due to the fact that humans are not a regular host of this parasite (Audicana et al., 2002;Klimpel et al., 2004).Through investigations of allergic responses to L3 for a period of more than 10 years, several shared features have been identified, which indicate that immune reactions to L3 infection evidence similar patterns in humans and experimental...

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Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

Section: Excretory-secretory Products Of a Simplex Larvaementioning

confidence: 99%

Section: Specific Immunoglobulin E Production and Allergy To L3mentioning

confidence: 99%

mentioning

confidence: 99%

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Immune reactions and allergy in experimental anisakiasis

Cho

Lee

2006

Korean J Parasitol

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A rat model of intragastric infection with Anisakis spp. live larvae: histopathological study

Zuloaga

Rodríguez-Bobada

Corcuera³

et al. 2013

Parasitol Res

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Anisakiasis is a fish-borne parasitic disease caused by consumption of raw or undercooked fish or cephalopods parasited by Anisakis spp. third stage larvae. The pathological effects of the infection are the combined result of the mechanical action of the larva during tissue invasion, the direct tissue effects of the excretory/secretory products released by the parasite, and the complex interaction between the host immune system and the Anisakis antigens. The aim of this study was to develop an experimental model of infection with Anisakis spp. live larvae in rats, useful to study the acute and chronic histopathological effects of the Anisakis infection. Sprague-Dawley rats were subjected to esophageal catheterization to place larvae directly into the stomach. Reinfections at different intervals after the first infection were preformed. Live larvae were found anchored to the mucosa and passing through the wall of the stomach and showed a strong resistance being able to stay alive at different sites and at the different pH. Migration of larvae from the stomach to other organs out of the gastrointestinal tract was also observed. The histopathological study showed the acute inflammatory reaction, with predominance of polymorphonuclear eosinophils and a mild fibrotic reaction. The model of infection described is valid to study the behavior of the larvae inside the host body, the histopathological changes at the invasion site, and the effects of the repeated infections by ingestion of live larvae.

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Molecular and immunological characterisation of tropomyosin from Anisakis pegreffii

et al. 2017

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Tropomyosin (TM) is a major allergen in shellfish, known to cross-react with mite, cockroach and/or some roundworm (nematode) TM. In this study, we aimed to express and purify TM from the parasitic nematode Anisakis pegreffii and also to characterise its cross-reactivity with TM from shellfish. A. pegreffii was isolated from the flathead tiger fish (Neoplatycephalus richardsoni) and characterised using single-strand conformation polymorphism (SSCP)-based sequencing of the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA. The recombinant tropomyosin (rTM) of A. pegreffii was expressed, purified and confirmed by immunohistochemistry, sequencing and LC-MS/MS analyses. Immunohistochemistry showed the muscle and the base layer of the third-stage larvae (L3) of A. pegreffii as the location of TM in A. pegreffii. The molecular relationship of TM of A. pegreffii with homologs from other nematodes and crustaceans was inferred from phylogenetic analysis. Immunogenicity of TM from A. pegreffii was tested by immunoblotting, which showed that rTM from A. pegreffii binds to IgE from sera of patients with allergy to crustaceans. Immunoblotting also showed that the anti-TM monoclonal antibody (MAb) did not recognise rTM from A. pegreffii. The rTM from A. pegreffii was, however, recognised by anti-TM polyclonal antibodies (PAbs) as well as anti-crustacean polyclonal antibodies (PAbs). The detection of specific serum IgE antibody against parasite TM has been proposed as a useful approach for the diagnosis of parasite-induced allergy. The findings of this study merit further exploration of the cross-reactive allergenic proteins of Anisakis for improved, future diagnosis of allergenic diseases.

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Immunochemical and Biological Analysis of Allergenicity with Excretory-Secretory Products of <i>Anisakis simplex</i> Third Stage Larva

Cited by 20 publications

References 44 publications

Immune reactions and allergy in experimental anisakiasis

Immune reactions and allergy in experimental anisakiasis

A rat model of intragastric infection with Anisakis spp. live larvae: histopathological study

Molecular and immunological characterisation of tropomyosin from Anisakis pegreffii

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