Reaginic Antibodies on Basophils in Grass Pollen Allergy, as Shown by Rosette-Formation

Wilson, Anne B.; Marchand, Rose-Mary; Wilson, D. V.; Devey, M. E.; Coombs, R.R.A.

doi:10.1159/000230647

Cited by 12 publications

(4 citation statements)

References 9 publications

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“…However, there is no correlation between mast cell ro settes and the reaginic content of sera, esti mated by PCA reactions. This discrepancy between cell-bound and circulating IgE has been reported and well documented in the rat [Jarrett and Stewart, 1973;Yamamoto et al, 1974] as well as in man [Aas and Jo hansson, 1971;Berg et al, 1971;Wilson et al, 1972]. This fact is of interest, inasmuch as clinical allergy mainly depends on cellbound reagins.…”

Section: Discussionmentioning

confidence: 87%

“…These rosettes result from ac tive sensitization and detect cytophilic anti bodies. Such rosettes have already been de scribed concerning macrophages [Boyden, 1964], basophils from guinea pigs [Wilson and Coombs, 1971] and basophils from al lergic individuals [Wilson et al, 1972]. Renoux et al [1975] described rosette-like aspects, when antihuman IgE-coated Sephadex granules were incubated with human IgE-coated rat mast cells.…”

Section: Discussionmentioning

confidence: 99%

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Rosette-Forming Mast Cells in Rat Anaphylaxis

Wallach¹,

Renoux²

1977

Int Arch Allergy Immunol

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Peritoneal mast cells from immunized rats can form rosettes with antigen-coated sheep red blood cells. The receptor responsible for this active rosette formation is shown to be IgE cytophilic antibody: rosettes are inhibited by previous contact of mast cells with antigen, or with anti-IgE antiserum; the kinetics of mast cell rosettes following a primary immunization with ovalbumin and Bordetella pertussis vaccine is similar to the kinetics of reaginic antibody response. Furthermore, a reaginic serum can induce passive rosette formation. There is no correlation between cell-bound and circulating IgE.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Rosette-Forming Mast Cells in Rat Anaphylaxis

Wallach¹,

Renoux²

1977

Int Arch Allergy Immunol

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“…To our knowledge, the only report based on the mast cell agglutination was made by Wii.son cl al. [16,17], who used an tigen-coupled erythrocytes in order to obtain an immune cyto-adhercnce with specifically sensitized basophiles. The use of antibody-coated Se-phadcx granules is another procedure which is rarely reported.…”

Section: Discussionmentioning

confidence: 99%

Binding of Human IgE Immunoglobulin to Rat Mast Cells

Renoux¹,

Montis²,

Roche³

et al. 1975

Int Arch Allergy Immunol

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The binding of human myeloma IgE immunoglobulin on rat mast cells was studied by three independent techniques. A mixed agglutination reaction with anti-IgE-coated Sephadex granules demonstrated that only human IgE-coated rat mast cells were clearly agglutinated. This binding is strong (50% agglutination) in 3 min and progresses for 30 min (95% agglutination). Autoradiographic studies with ¹²⁵I-labelled human serum proteins demonstrated the selective formation of grains on mast cells incubated with labelled IgE. Upon action of anti-IgE antiserum on IgE-coated rat mast cells, the mast cells released up to 47.5%) of their total histamine content in a fluorometric histamine assay. A relationship was established between sensitizing doses of human IgE and histamine release. These results bring evidence for a binding of human IgE on rat mast cells and imply the existence of receptors for this immunoglobulin on mast cell membrane.

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“…Methods have been reported to detect a specific allergen by the response of blood basophils to the allergen (Wilson, Marchand & Coombs, 1971;Wilson et al, 1972;Hirsch & Zastrous, 1972;Soifer & Hirsch, 1975;King, Alaye & Augustin, 1976). We described previously an indirect method in which the morphological changes of blood basophils following the addition of anti-IgE were observed on smear preparations (Kimura et al, 1975b).…”

Section: Introductionmentioning

confidence: 99%

New in vitro method for detecting asthma allergen

Kimura

Tanizaki

Goda

et al. 1983

Clin Experimental Allergy

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Summary Changes in the morphology and number of basophils were examined after the addition in vitro of house dust extract in cells from seventy‐seven patients with bronchial asthma. Morphological changes (reactive basophils) showed a close relation to the end‐point titrations of antigen in skin tests, RAST score and bronchial provocation tests, but changes in number of basophils did not. With a RAST score of 2 or greater the change in reactive basophils varied from 40 to 80% (mean 58‐1%), which was much greater than seen in persons with no RAST, score 0. Patients with positive bronchial inhalation tests, showed 40 to 80% (mean 57.8%) reactive basophils, whereas persons with a negative bronchial challenge test showed a range of reactive basophils from 0 to 50% (mean 28.9%). The difference in percentage of reactive basophils between the positive and negative groups of persons tested by bronchial inhalation was statistically significant.

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Reaginic Antibodies on Basophils in Grass Pollen Allergy, as Shown by Rosette-Formation

Cited by 12 publications

References 9 publications

Rosette-Forming Mast Cells in Rat Anaphylaxis

Rosette-Forming Mast Cells in Rat Anaphylaxis

Binding of Human IgE Immunoglobulin to Rat Mast Cells

New in vitro method for detecting asthma allergen

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