Detection of
            <i>qnr</i>
            in Clinical Isolates of
            <i>Escherichia coli</i>
            from Korea

Jeong, Jin-Yong; Yoon, Haesung; Kim, Eun Sil; Lee, Yoola; Choi, Sang‐Ho; Kim, Nam Joong; Woo, Jun Hee; Kim, Yang Soo

doi:10.1128/aac.49.6.2522-2524.2005

Cited by 71 publications

(56 citation statements)

References 11 publications

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“…According to Jeong et al (2005), the presence of a qnr determinant increases resistance to nalidixic acid and fluoroquinolones four to eight times [30]. In our study, the majority of ciprofloxacin-resistant isolates carrying qnr genes showed complete resistance to ciprofloxacin (MICs > 32 μg/mL).…”

Section: Discussionmentioning

confidence: 67%

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh

Zibaei

Soleimani-Asl

2014

J Infect Dev Ctries

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Introduction: Plasmid-mediated quinolone resistance, which complicates treatment, has been increasingly identified in Escherichia coli isolates worldwide. The purpose of this study was to identify the plasmid-mediated qnrA and qnrB genes among the quinolone-resistant Escherichia coli isolated from urinary tract infections in Iran. Methodology: A total of 140 Escherichia coli isolates were collected between March and October 2012 from urinary tract infections in Khorram Abad, Iran. All isolates were tested for quinoloe resistance using the disk diffusion method. Also, all quinolone-resistant isolates were screened for the presence of the qnrA and qnrB genes by polymerase chain reaction. Minimum inhibitory concentrations (MICs) of ciprofloxacin for the qnr-positive isolates were determined. Results: One hundred sixteen (82.8%) of 140 Escherichia coli isolates were nalidixic acid-resistant; among them, 14 (12.1%) and 9 (7.8%) were qnrA and qnrB-positive, respectively. Two quinolone-resistant isolates harbored both qnrA and qnrB. Among 63 ciprofloxacin-resistant isolates, 14 (22.2%) and 9 (14.3%) were found to carry qnrA and qnrB genes, respectively. The ciprofloxacin MIC range was 0.25-512 μg/mL for 23 qnr-positive Escherichia coli isolates, 18 of which had MICs values of 4-512 μg/mL. Conclusion: Our study shows that the frequency of plasmid-mediated quinolone resistance genes among E. coli isolates in Iran is high.

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Section: Discussionmentioning

confidence: 67%

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh

Zibaei

Soleimani-Asl

2014

J Infect Dev Ctries

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“…Between 2001 and 2003, the prevalence of qnr in Korea was less than 1% [16]. However, this value has rapidly increased since then.…”

Section: Discussionmentioning

confidence: 99%

“…To determine whether the aac(6′ )-Ib-cr-carrying plasmid is transferable, conjugation experiments were performed using azide-resistant E. coli J53 as the recipient [16,17]. Each donor and recipient strain was inoculated into brain heart infusion broth (Difco, Cockeysville, MI, USA) and shake culture was performed for 3 hr after inoculation.…”

Section: Conjugation Experimentsmentioning

confidence: 99%

Characteristics of aac(6′)-Ib-cr Gene in Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Chungnam Area

et al. 2009

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hibitory concentrations (MICs) of ciprofloxacin, tobramycin, and amikacin and indicated the statistically significant roles of qnrB, qnrS, rmtA, and rmtB in quinolone and aminoglycoside resistance.Conclusions : The aac(6′ )-Ib-cr variants were widespread and showed significant relation to the highlevel quinolone and aminoglycoside resistance in ESBL-producing E. coli and K. pneumoniae. (Korean J Lab Med 2009;29:541-50)

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“…In particular, FQ, a broad spectrum antimicrobial agent effective against most bacteria, has been widely used in the treatment of diseased cattle in Korea. While there has been a number of studies on the mechanisms of FQ resistance in E. coli that have originated from human clinics in Korea [3,9], no study has been conducted to elucidate FQ resistance mechanisms in E. coli isolates from animals, except chickens, in Korea [11]. The aim of the present study was therefore to determine the prevalence and mechanisms of FQ resistance in E. coli isolates from diarrheic beef cattle in Korea.…”

mentioning

confidence: 99%

Prevalence and Molecular Characterization of Fluoroquinolone-Resistant Escherichia coli Isolated from Diarrheic Cattle in Korea

Lim

Lee

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. A total of 176 E. coli isolates were retrieved from 203 diarrheic fecal samples collected from Korean cattle on 117 different farms. The most frequently observed resistance in E. coli isolates was to tetracycline (88.6%), followed by streptomycin (80.7%) and ampicillin (64.8%). Resistance to cefazolin, cefoperazone, cefepime and amikacin was very low. Of the 176 E. coli strains, forty (22.7%) isolates from 30 farms showed resistance to fluoroquinolones (FQ). All the FQ-resistant strains possessed double mutations at codons 83 and 87 in the gyrA gene, and a single mutation mostly at codon 80 in the parC gene, except in one isolate. The pulsed-field gel electrophoresis profiles of the FQ-resistant E. coli isolates were heterogeneous, but two or three isolates that showed an identical pattern originated from the same or different farms. This study demonstrates that FQ resistance is frequently observed in E. coli from diarrheic cattle and that mutations in the quinolone resistance-determining region are the same as those seen in E. coli originating from other animal species and humans. The FQ resistance in diarrheic cattle might have been mostly acquired independently, although the possibility of transmission of FQ-resistant E. coli within a farm or between farms is plausible.

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Detection of qnr in Clinical Isolates of Escherichia coli from Korea

Cited by 71 publications

References 11 publications

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Characteristics of aac(6′)-Ib-cr Gene in Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Chungnam Area

Prevalence and Molecular Characterization of Fluoroquinolone-Resistant Escherichia coli Isolated from Diarrheic Cattle in Korea

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