Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh, Farzaneh; Zibaei, Mohammad; Soleimani-Asl, Younes

doi:10.3855/jidc.3746

Cited by 22 publications

(25 citation statements)

References 28 publications

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“…In a study conducted on quinolone resistance amongst E. coli strains isolated from UTI by Firoozeh et al, (2013) frequencies of qnrA and qnrB in nalidixic acid-resistant isolates were 12.1% and 7.8% and also in ciprofloxacin-resistant isolates, these were 22.2% and 14.3%, respectively (33). However, in the present study, the prevalence of qnrA and qnrB was 3.3% and 1.4% respectively.…”

Section: Discussioncontrasting

confidence: 50%

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Goudarzi

Fazeli

2017

Shiraz E-Med J

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Background: Prevalence of plasmid mediated quinolone resistance in Escherichia coli clinical isolates is a serious problem in developing countries. Objectives:The aim of this study was to investigate quinolone resistance determinants among Extended-Spectrum B-Lactamases (ESBL)-producing E. coli recovered from patients with nosocomial urinary tract infection. Methods: A total of 290 E. coli isolates, obtained from patients with UTI, were included in this study. The phenotypic confirmatory test for ESBL production was performed by double disc synergy test and combined disk diffusion test. Kirby-Bauer Disk diffusion method was performed to test susceptibilities of ESBL-producing E. coli isolates to 15 antimicrobial agents. Isolates were screened for the presence of ESBL and plasmid-mediated quinolone resistance genes by polymerase chain reaction (PCR). Results: In the present study, 290 (71.7%) E. coli strains were recovered from 410 hospitalized patients with UTI, 51.7% of which were found to have ESBL positive results. In vitro, the susceptibilities of ESBL-producing E. coli strains showed that all isolates were resistant to amoxicillin and penicillin and resistance rates to other of antibiotics differed from 40% to 96%. Among the 150 ESBL positive isolates, frequencies of aac(6')- Ib, oqxA, oqxB, qnrA, qnrB, qnrS, and qepA were 74.7%, 8%, 4%, 3.3%, 1.3%, 2%, and 2.7%, respectively. Coexistence of bla CTX-M, bla TEM, bla CTX-M, and aac(6')-Ib were the most widely distributed resistance genotypes. Conclusions:The data of the present study revealed the high prevalence of plasmid-mediated quinolone resistance genes among ESBL-producing E. coli in the hospitals. The bla CTX-M genes were found to be the dominant ESBL-encoding gene.

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Section: Discussioncontrasting

confidence: 50%

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Goudarzi

Fazeli

2017

Shiraz E-Med J

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“…Quinolones are the most widely used antibacterial agents in fighting against serious infections caused by E. coli and other members of Enterobacteriaceae in Iran [19]. However, plasmid-mediated quinolone resistance in the genus belonging to Enterobacteriaceae, especially E. coil , has led to treatment failures and currently is becoming a significant public health concern.…”

Section: Discussionmentioning

confidence: 99%

“…Our results were partly similar to the resistance levels reported in two previously conducted studies in Iran. Firoozeh et al [19] reported that 82.5% and 45% of urinary E. coli isolates were resistant to nalidixic acid and ciprofloxacin, respectively. In another study from Iran, Khorvash et al [21] found that 76% and 52% E. coli isolates associated with nosocomial infection were resistant to nalidixic acid and ciprofloxacin, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The frequency of qnr genes in our study was lower than those found in the two studies previously conducted in Iran. In a study from Khorramabad (Iran), Firoozeh et al reported that 14 (12.1%) and nine (7.8%) nalidixic acid-resistant E. coli isolates were positive for qnrA and qnrB genes, respectively [19]. In another study from Tehran, Pakzad et al [24] showed that qnrA and qnrB genes were present in 37.5% and 20.8% of ESBL-producing E. coli isolates, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Plasmid-Mediated Quinolone-Resistance (qnr) Genes in Clinical Isolates of Escherichia coli Collected from Several Hospitals of Qazvin and Zanjan Provinces, Iran

Rezazadeh

Baghchesaraei

Peymani

2016

Osong Public Health and Research Perspectives

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ObjectivesEscherichia coli is regarded as the most important etiological agent of urinary tract infections. Fluoroquinolones are routinely used in the treatment of these infections; however, in recent years, a growing rate of resistance to these drugs has been reported globally. The aims of this study were to detect plasmid-mediated qnrA, qnrB, and qnrS genes among the quinolone-nonsusceptible E. coli isolates and to investigate their clonal relatedness in Qazvin and Zanjan Provinces, Iran.MethodsA total of 200 clinical isolates of E. coli were collected from hospitalized patients. The bacterial isolates were identified through standard laboratory protocols and further confirmed using API 20E test strips. Antimicrobial susceptibility was determined by the standard disk diffusion method. Polymerase chain reaction (PCR) and sequencing were used for detecting qnrA, qnrB, and qnrS genes and the clonal relatedness of qnr-positive isolates was evaluated by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) method.ResultsIn total, 136 (68%) isolates were nonsusceptible to quinolone compounds, among which 45 (33.1%) and 71 (52.2%) isolates showed high- and low-level quinolone resistance, respectively. Of the 136 isolates, four (2.9%) isolates were positive for the qnrS1 gene. The results from ERIC-PCR revealed that two (50%) cases of qnr-positive isolates were related genetically.ConclusionOur study results were indicative of the presence of low frequency of qnr genes among the clinical isolates of E. coli in Qazvin and Zanjan Provinces, which emphasizes the need for establishing tactful policies associated with infection-control measures in our hospital settings.

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“…Over one million cases of CAUTIs are reported annually, and CAUTIs account for about 80% of all nosocomial UTIs cases [3]. Escherichia coli is the most common cause of UTIs, accounting for 80%-90% of all UTIs seen among outpatients [4]. Uropathogenic Escherichia coli (UPEC) strains are known as the most frequent causative agent of UTIs [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Firoozeh

Soleimani-Moorchekhorti

Zibaei

2017

J Infect Dev Ctries

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Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536 were not detected in the UPEC strains. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.

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Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Cited by 22 publications

References 28 publications

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Plasmid-Mediated Quinolone-Resistance (qnr) Genes in Clinical Isolates of Escherichia coli Collected from Several Hospitals of Qazvin and Zanjan Provinces, Iran

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

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