Characteristics of aac(6′)-Ib-cr Gene in Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Chungnam Area

Shin, So Youn; Kwon, Gye Cheol; Park, Jong Woo; Song, Jeong‐Hoon; Ko, Young Hyun; Sung, Ji Youn; Shin, Hae Won; Koo, Sun Hoe

doi:10.3343/kjlm.2009.29.6.541

Cited by 38 publications

(22 citation statements)

References 26 publications

(17 reference statements)

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“…In addition, qnrA gene enhances the selection of chromosomal encoded quinolone resistance determinants which confer additional resistance to fluoroquinolones. Other plasmid-mediated quinolone resistance determinants qnrB (qnrB1 to qnrB6) and qnrS (qnrS1 and qnrS2) have been also identified in enterobacterial species, sharing 41 and 60% amino acid identity with qnrA, respectively (Nordmann and Poirel, 2005;Shin et al, 2009). Another mechanism of quinolone resistance relies on upregulation of efflux pump which exports El-Sokkary and Abdelmegeed 663 quinolones and other antimicrobials out of the bactereial cell.…”

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confidence: 99%

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

El‐Sokkary¹,

Abdelmegeed²

2015

Afr. J. Microbiol. Res.

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Extensive use of fluoroquinolone antibacterial in clinical practice has been associated with increasing frequency of quinolone-resistant Escherichia coli strains. In the current study, a total of 80 E. coli clinical isolates from Mansoura hospitals patients in Egypt were studied for antibacterial susceptibility pattern against 15 different antibacterials. These strains were tested for quinolones resistance by minimum inhibitory concentration (MIC) determination using broth micro-dilution method. The resistance rate of ciprofloxacin and levofloxacin for E. coli isolates was found to be 60%. PCR was performed for detection of plasmid-mediated quinolone resistance genes including qnrA, qnrB and qnrS. 30 and 61.3% of E. coli isolates were positive for qnrA and qnrB, respectively, whereas qnrS was identified in only 15% of isolates. Quinolone resistance-determining region (QRDR) of gyrA and ParC genes was characterized for 17 ciprofloxacin and levofloxacin resistant E. coli isolates (MIC 12.5-200 µg mL -1 ). Two mutation sites in gyrA were detected in 17 tested E. coli isolates. However, two mutation sites in parC were detected in four E. coli isolates. The amino acid change at Ser-83 and aspartic-87 in GyrA were the most common mutation sites identified in the isolates. These results indicated that multiple mechanisms of quinolone-resistance are commonly found in E. coli isolated from Mansoura hospitals.

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Section: Introductionmentioning

confidence: 99%

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

El‐Sokkary¹,

Abdelmegeed²

2015

Afr. J. Microbiol. Res.

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“…Comparative genomic analysis of plasmid pKOX3-P2-OXA identified a novel class 1 integron, i.e., intl1-[aac(6=)-lb-cr]-bla OXA-10 -aadA1-sul1. The aac(6=)-Ib-cr gene has been reported to be associated with ESBL, oqx, or qnr genes in Enterobacteriaceae (12,13). AAC(6=)-Ib-cr, as a variant aminoglycoside acetyltransferase, is prevalent and has been mapped to various plasmids, transposons, and integrons in Enterobacteriaceae (13,14).…”

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First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

Wang

Yuan

Chen

et al. 2017

Antimicrob Agents Chemother

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The nucleotide sequences of five plasmids from one Klebsiella oxytoca isolate were determined using the PacBio RS II system. Plasmid analysis revealed that bla NDM-1 was carried on an IncX3 plasmid. The bla IMP-4 and bla KPC-2 genes were located on IncN and IncP-6 plasmids, respectively. Comparative sequence analysis highlighted the successful spread of carbapenemase-harboring plasmids among different enterobacterial species. We report for the first time, to our knowledge, coproducing NDM-1, KPC-2, and IMP-4 carbapenemases on a K. oxytoca isolate.

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“…Sequencing of PCR products with primers for bla CTX-M-9 -related genes (1) and TEM-FH (5=-CAATAAGCTTCAAAAAGGAAGAG T-3=) and TEMXhoR (5=-GTGCTCGAGCCAATGCTTAATCAG TG-3=) confirmed the presence of bla CTX-M-14 and bla TEM-1 , respectively. Conjugation assays were carried out using Escherichia coli J53-2 (rifampin resistant) as the recipient; transconjugants were selected on MacConkey agar plates supplemented with rifampin (150 mg/liter) and ceftriaxone (1 mg/liter) (26). SE1101 transconjugants (TcSE1101) showed a resistance profile similar to that of the donor strain and positive PCR results for bla CTX-M and bla TEM (Table 1).…”

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First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla _CTX-M-14 in South America

Bado

García-Fulgueiras

Cordeiro

et al. 2012

Antimicrob Agents Chemother

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eWe studied a clinical isolate of Salmonella enterica serotype Enteritidis showing resistance to oxyiminocephalosporins. PCR analysis confirmed the presence of bla CTX-M-14 linked to IS903 in a 95-kb IncI1 conjugative plasmid. Such a plasmid is maintained on account of the presence of a pndAC addiction system. Multilocus sequence typing (MLST) analysis indicated that the strain belongs to ST11. This is the first report of bla CTX-M-14 in Salmonella Enteritidis of human origin in South America.

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Characteristics of aac(6′)-Ib-cr Gene in Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Chungnam Area

Cited by 38 publications

References 26 publications

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla _CTX-M-14 in South America

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Characteristics of aac(6′)-Ib-cr Gene in Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Chungnam Area

Cited by 38 publications

References 26 publications

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

Antibacterial resistance pattern among Escherichia coli strains isolated from Mansoura hospitals in Egypt with a special reference to quinolones

First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla CTX-M-14 in South America

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First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla _CTX-M-14 in South America