Detection of Human Papillomavirus Type 60 in Plantar Cysts and Verruca Plantaris by the In situ Hybridization Method Using Digoxigenin Labeled Probes

Abstract

At first, punctate keratotic lesions are induced by HPV 60. HPV 60 induces irregular elongation of the rete ridges into the dermis. Plantar cysts probably form as a result of the traumatic implantation of HPV 60 infected epidermis with irregularly long and narrow rete ridges into the dermis.

Analysis of the mononuclear inflammatory cell infiltrate in the nontumorigenic, Pretumorigenic and tumorigenic keratinocytic hyperproliferative lesions of the skin

“…8 Alternatively, in the nontumorigenic lesions, these antigens include the viral antigens such as human papillomavirus antigens (VV), bacterial superantigens (LP), HLA antigens, cutaneous lymphocyte-associated antigen (PV). 9,10 Moreover, the damaged keratinocytes might produce more cytokines that recruit more immunocytes. 11 The gradual increase in B cell count from non-tumorigenic, to pre-tumorigenic, and finally to tumorigenic lesions go in line with other groups.…”

A Case of a Human-Papillomavirus-60-Induced Wart with Clinical Appearance of Both Pigmented and Ridged Warts

“…The case described here is interesting because the wart had features of both pigmented and ridged warts, suggesting that ridged warts can be pigmented. A case of HPV-60-induced wart reported by Kawase et al [10]also seems to retain a ridged pattern on the smooth surface of a flat pigmented lesion. In our case, it was demonstrated that increased melanin granules in both keratinocytes and melanin blockade melanocytes were the primary contributors to clinical pigmentation.…”

“…Vacuolated cells were also seen in the granular cell layers. To determine the causative HPV type, in situ hybridization was applied to formalin-fixed, paraffin-embedded sections using digoxigenin-labelled HPV-1, -2, -4, -60, -63 and -65 DNA probes, as described elsewhere [10]. Positive signals in the nuclei of the keratinocytes distributed mainly in the upper part of the epidermis were only seen when HPV-60 DNA was used as a probe (fig.…”

Genetics of Epidermodysplasia Verruciformis

“…Histopathologically, lesions demonstrate stereotypical enlarged keratinocytes in upper epidermis with gray-blue cytoplasm, enlarged round nuclei with pale chromatin, and one or multiple nucleoli (Figure 3).The Immunohistochemistry findings showed the HPV antigens [26].In electron microscopy, The nuclei are clarified with maeginated chromatin, and crystalline viral particles are present in nucleoplasm and in the prominent nucleoli ( Figure 6). Under an electron microscope, HPV5 virions purified from pooled scales of EV patients and virus-like particles (VLP) assembled from a purified recombinant baculoviruses expressing the L1 major capsid protein of HPV5 were observed( Figure 7) [27].…”