Detection of Human Papillomavirus in Normal Oral Cavity in a Group of Pakistani Subjects using Real-Time PCR

Gichki, Abdul Samad; Buajeeb, Waranun; Doungudomdacha, Sombhun; Khovidhunkit, Siribang-On Pibooniyom

doi:10.7314/apjcp.2012.13.5.2299

Cited by 29 publications

(32 citation statements)

References 19 publications

(18 reference statements)

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“…Several studies have found that sexual behavior (i.e., marital status, number of sex partners, oral sex, and anal sex), smoking, and alcohol drinking are significant risk factors for oral HPV infection [10-13, 20, 23, 30, 31, 33]. Therefore, we performed a meta-analysis to investigate the association between overall oral HPV infection and outcome, as described above.…”

Section: Resultsmentioning

confidence: 99%

Risk Factors for Oral Human Papillomavirus Infection in Healthy Individuals: A Systematic Review and Meta-Analysis

Shigeishi

Sugiyama

2016

J Clin Med Res

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BackgroundOral human papillomavirus (HPV) infection is associated with oral cancer development. However, few epidemiologic investigations have focused on oral HPV prevalence in healthy individuals. The objective of this study was to provide updated information regarding oral HPV prevalence in patients without oral cancer worldwide.MethodsWe systematically reviewed 29 studies reporting the prevalence of oral HPV infection that included 22,756 subjects (10,124 males, 12,623 females, and nine unknown gender; age range 2 - 89 years) and were published from January 2012 to June 2015.ResultsThe prevalence of overall HPV, low-risk type HPV, high-risk type HPV, and HPV16 in the reported cases was 5.5%, 2.2%, 2.7%, and 1.0%, respectively. The prevalence of overall HPV was considerably higher in males who had sex with males (12.2%) as compared to heterosexual males (4.7%) and females (2.9%). A meta-analysis was performed to elucidate significant risk factors for oral HPV infection, which revealed a significant statistical association for oral sex and smoking with oral HPV infection (odds ratio (OR): 1.90, 95% confidence interval (CI): 1.51 - 2.39, P < 0.0001; OR: 2.13, 95% CI: 1.32 - 3.43, P = 0.002).ConclusionsOur findings suggest that sexual behavior and smoking are importantly related to oral HPV infection in healthy individuals.

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Section: Resultsmentioning

confidence: 99%

Risk Factors for Oral Human Papillomavirus Infection in Healthy Individuals: A Systematic Review and Meta-Analysis

Shigeishi

Sugiyama

2016

J Clin Med Res

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“…This low prevalence of HPV in the oral cavity without any lesions was due to the salivary immune response which includes IgA, cystatins, and proteolytic enzymes that protect the oral mucosa. [3] Other studies by Giovannelli et al .,[16] Gichki et al .,[17] Miller et al . [18] and Sikka et al .…”

Section: Discussionmentioning

confidence: 99%

Expression of human papillomavirus dna and p53 polymorphisms through polymerase chain reaction in normal mucosa and oral leukoplakia individuals with deleterious oral habits

Ramya

Majumdar

Babu

et al. 2017

Int J App Basic Med Res

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Context:Oral leukoplakia (OL) is a well-recognized precancerous lesion with various etiological factors. Most commonly deleterious oral habits such as tobacco smoking and viral etiologies mainly human papillomavirus (HPV) play an important role. p53 polymorphisms mostly homozygous Arginine (Arg) allele has a greater risk of degradation by HPV. Hence, HPV infection and p53 polymorphisms may act as synergistic factors for increased the risk of malignant transformation in oral precancerous lesions.Aims:The aim of this study is to evaluate the risk of OL and its malignant transformation due to infection by HPV and p53 polymorphisms in the oral biopsy samples through polymerase chain reaction (PCR).Subjects and Methods:A total of 40 individuals were involved– 10 individuals were controls without deleterious habits, 15 were controls with deleterious habits, and 15 were with histologically confirmed OL individuals with deleterious habits. PCR and restriction fragment length polymorphism using sma1 enzyme were carried out to evaluate the expression of HPV and p53 polymorphisms.Statistical Analysis Used:Chi-square test, Fischer's exact t-test, and odds ratio.Results:(1) HPV DNA expression was higher in Leukoplakia individuals than controls. (2) p53 genotype with homozygous Arg was more in HPV-infected individuals.Conclusions:To conclude HPV infected OL cases were mostly with Arg/Arg type of p53 polymorphism.

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“…Recently, more than 100 HPV types have been classified into low and high risk groups according to their viral expression in malignancies. HPV16 and 18 are categorized into the high-risk group as they are associated with several malignancies including cervical and oral cancers (Gichki, 2012).…”

Section: Introductionmentioning

confidence: 99%

Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

Heah¹,

Kwai-Lin²,

Froemming³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective: This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and Methods: Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result: From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion: The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.

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Detection of Human Papillomavirus in Normal Oral Cavity in a Group of Pakistani Subjects using Real-Time PCR

Cited by 29 publications

References 19 publications

Risk Factors for Oral Human Papillomavirus Infection in Healthy Individuals: A Systematic Review and Meta-Analysis

Risk Factors for Oral Human Papillomavirus Infection in Healthy Individuals: A Systematic Review and Meta-Analysis

Expression of human papillomavirus dna and p53 polymorphisms through polymerase chain reaction in normal mucosa and oral leukoplakia individuals with deleterious oral habits

Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

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