Expression of human papillomavirus dna and p53 polymorphisms through polymerase chain reaction in normal mucosa and oral leukoplakia individuals with deleterious oral habits

Ramya, A.; Majumdar, Swapan; Babu, T. M. Suneel; Uppala, Divya; Srinivas, Baratam; Rao, Ayyagari Kameswara

doi:10.4103/ijabmr.ijabmr_57_16

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…It seems that the TP53 protein with Pro form (p53‐Pro72) is less susceptible to the E6‐mediated degradation in comparison to the Arg (p53‐Arg72) form. This was reported by Babakhani studying TP53 polymorphism with HPV infection in prostate cancer and confirmed by similar results from Ramya, Hou, Storey, and Nagpal …”

Section: Discussionsupporting

confidence: 87%

Mucosal human papillomavirus detection and TP53 immunohistochemical expression in non‐melanoma skin cancer in Tunisian patients

et al. 2019

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Background: Several studies have reported the oncogenic role of human papillomavirus (HPV) in non-melanoma skin cancer (NMSC) carcinogenesis.Considering that HPV could affect tumor protein 53 (TP53) degradation via E6 oncoprotein, we evaluated the expression of TP53 according to HPV infection and E6 expression. Methods: Biopsy specimens from 79 NMSCs (28 squamous cell carcinomas, 21 keratoacanthomas and 30 basal cell carcinomas) were enrolled. Nested PCR was used to detect mucosal HPV (mHPV) DNA. Genotyping was performed by reverse line hybridization. Expression of TP53 and E6 was evaluated by immunohistochemistry. Results: mHPVs were detected in 34.2% (27/79) of NMSC, with 92.6% (25/27) of high-risk HPV (HR-HPV) types. HPV16-E6-positive expression was observed in all HPV16-positive samples. TP53 high expression was found in 51.4% (37/72) of specimens. In this group, 78.4% were HPV-negative (P = 0.014). TP53 expression was negative in 8/10 of HPV E6-positive specimens.Multivariate analysis showed that TP53 was associated with HPV infection independently of histopathologic type (P = 0.005).Conclusion: This study showed a high prevalence of mHPV in NMSC. Active infections assessed by E6 expression are associated with loss of p53 function, highlighting the involvement of mHPV in NMSC carcinogenesis. K E Y W O R D SHPV16-E6s, mucosal human papillomavirus, non-melanoma skin cancer, TP53, Tunisian

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Section: Discussionsupporting

confidence: 87%

Mucosal human papillomavirus detection and TP53 immunohistochemical expression in non‐melanoma skin cancer in Tunisian patients

et al. 2019

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“…Among these 26 manuscripts, 13 were excluded due to not pertinent full text (11 studies with no OLK subdivision in a study group or no subdivided outcomes, even the number of OLK cases was known, 2 studies with no OLK). There were 6 articles with casecontrol studies [5][6][7][8][9][10], 7 articles with cross-sectional studies [11][12][13][14][15][16][17] and no articles with cohort studies.…”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, outcomes were analysed to estimate overall prevalence in the group without and with dysplasia. Dysplasia of leukoplakia was mentioned in 7 articles [5,7,9,11,14,16,17]. Four articles were excluded due to lack of HPV detected in some study or not-subdivid- There were limitations of some analysed studies associated with HPV-DNA detection: high risk of false outcomes due to non-quantitative PCR and PCR-product visualization on gel [5,7,8,13,14], and a very small group of OLK [6,10,13].…”

Section: Resultsmentioning

confidence: 99%

Human papillomavirus and leukoplakia of the oral cavity: a systematic review

Radzki¹,

Kusiak²,

Ordyniec-Kwaśnica³

et al. 2022

pdia

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Introduction: Human papillomavirus (HPV) infection is mainly a problem of the female reproductive tract. It can occur in the oral cavity as well. Commonly HPV infections are subclinical, without any visible symptoms, and last no longer than 2 years. Usually the clinical manifestation of HPV infection is benign, but in some cases it can also promote malignant transformation. In the paper we have tried to estimate the prevalence of HPV detected in samples of oral leukoplakia (OLK), the most common premalignant lesions of the oral mucosa. Aim: To review the current literature to estimate the prevalence of HPV (HPV DNA) detected in samples of oral leukoplakia. Material and methods: We searched PubMed/Medline, Scopus, and Cochrane Library databases for studies that examined the prevalence of HPV in leukoplakia with HPV DNA detection by polymerase chain reaction. Results: HPV positive cases in OLK ranged from 0% to 100% in studies. The overall HPV prevalence in leukoplakia was 6.66%. The prevalence of HPV 16 positive cases was 2.95%. The high-risk HPV prevalence was 5.16%, when the low-risk HPV prevalence was 3.32%. When dysplasia is mentioned, HPV was detected in 25.93% of lesions with dysplasia, compared to 37.14% among non-dysplastic lesions. Conclusions: Further studies should be extended also to include low-risk HPV and compare its prevalence with presence of dysplasia in leukoplakia. Besides, leukoplakia and other premalignant lesions can no longer be treated as one lesion.

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“…5 The ethiological factors of OL is various as human papilloma virüs (HPV), tobacco smoking. 6 Enhancer of zeste homolog 2 (EZH2) is a protein involved in the regulation of cell cycle. Researches on normal oral mucosa, dysplasia and OSCC showed progressively higher expressions of EZH2, which was found to be associated with the potential of malignancy and poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

Expressions of Igf-1r, Ezh2, Laminin-5 in Leukoplakia and Oral Squamous Cell Carcinoma

Güner¹,

Güler²,

Kayhan³

et al. 2021

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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The aim of this study is to investigate the expressions of IGF-1R, EZH2 and Laminin-5 antibodies in biopsy samples of leukoplakia and squamous cell carcinoma of the oral mucosa by immunohistochemistry method. Materials and Methods: The study consisted of three groups; oral squamous cell carcinoma leukoplakia and control groups. The control group consisted of lesions with oral fibrous hyperplasia diagnosis. Biopsies of 38 patients for oral squamous cell carcinoma, 32 patients for leukoplakia and 15 patients for control group were examined and evaluated degree of staining of antibodies. Antibodies were evaluated as negative (0-10%):0, 1 positive (11-30%):+, 2 positive (31-60%):++, 3 positive (61-100%):+++ according to staining percentages. The staining degrees of antibodies were compared with Mann Whitney U test in study and control groups. Gender distribution between groups was compared with Chi-Square test and SPSS 21 program was used for calculations. The results were statistically significancant is p ˂0.05. Results: According to the findings obtained in this study antibodies revealed significantly higher staining in the oral squamous cell carcinoma group and leukoplakia group compared to with control groups. Conclusion:In this study, it was shown that EZH2, IGF-1R and Laminin-5 may have roles in cancer development.

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Expression of human papillomavirus dna and p53 polymorphisms through polymerase chain reaction in normal mucosa and oral leukoplakia individuals with deleterious oral habits

Cited by 9 publications

References 22 publications

Mucosal human papillomavirus detection and TP53 immunohistochemical expression in non‐melanoma skin cancer in Tunisian patients

Mucosal human papillomavirus detection and TP53 immunohistochemical expression in non‐melanoma skin cancer in Tunisian patients

Human papillomavirus and leukoplakia of the oral cavity: a systematic review

Expressions of Igf-1r, Ezh2, Laminin-5 in Leukoplakia and Oral Squamous Cell Carcinoma

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