Detection of human herpesvirus 6 and 7 DNA in saliva from healthy adults from Rio de Janeiro, Brazil

Magalhães, Ivna M; Martins, Rebeca Vazquez Novo; Cossatis, João José; Cavaliere, Renata M; Afonso, Larissa Alves; Moysés, Natalia; Oliveira, Solange Artimos de; Cavalcanti, Sílvia M. B.

doi:10.1590/s0074-02762010000700015

Cited by 19 publications

(23 citation statements)

References 24 publications

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“…In our study, we have also noticed a dominance of HHV6-B over HHV6-A, in accordance to the findings presented by Ward (2005). It is interesting to notice that a previous study from our group showed the opposite profile in healthy subjects, in which these agents are often shed without clinical signs (Magalhães et al 2010). Nevertheless, due to the small sample studied, divergences were expected.…”

Section: Discussionsupporting

confidence: 92%

“…A multiplex nested PCR assay was used in order to detect HHV-6A, B and HHV-7 as previously described by Magalhães et al 2010. Briefly, DNA was extracted from 500 μl of whole saliva using Invisorb DNA mini kit (Invitek, Germany).…”

Section: Hhv6b and Hhv7mentioning

confidence: 99%

“…In a previous study to evaluate the saliva of healthy adults in the state of Rio de Janeiro, the prevalence of both HHV-6 and 7 ranged from 9.8% to 12.6% (Magalhães et al 2010). Nevertheless, little is known concerning Roseolovirus shedding in post-transplant patients, especially in long-term ones, among whom indirect effects such as persistent fever, anemia, chronic fadigue syndrome among others are frequent and can compromise life quality.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Human Herpesviruses 6 and 7 Dna in the Saliva of Renal Transplanted Patients and Healthy Individuals From Rio De Janeiro, Brazil

Martins¹,

Cossatis²,

Afonso³

et al. 2011

VR&R

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In this study, we have sought to verify the prevalence ofhuman herpesviruses 6 and 7 (HHV-6, HHV-7) in the saliva of renal transplanted patients from Rio de Janeiro State, Brazil, and compare results with those of healthy subjects, since Roseolovirus DNA detection in body fluids from transplanted patients has been associated with often misdiagnosed chronic symptoms, organ rejection and even death. The studied group was composed by 120 individuals: 60 were renal transplanted patients and 60 were healthy subjects attending the Hospital Universitário Pedro Ernesto, for odontological follow-up. Saliva specimens were submitted to a multiplex nested polymerase chain reaction (PCR) to detect the presence of HHV-6A, HHV-6B and HHV-7. The total Roseolovirus DNA prevalence was 56.7% for transplanted patients and 23.3% for healthy individuals (p<0.001). For immunossupressed patients, the PCR detected a HHV-6A prevalence of 16.7% in transplanted, HHV-6B in 26.6% and HHV-7 DNA was revealed in 13.3% of the studied cases. In healthy subjects, HHV-6A was found in 5% of the samples, HHV-6B in 6.7% and HHV-7 in 11.7%. Multiple infections were observed in 12/60 (20%) individuals. No co-infection was demonstrated for healthy subjects, reinforcing the idea that imunnossuppression can favor reactivation and possibly transactivation among herpesviruses (P<0.001). Statistically significant differences were recorded for HHV6A and B infections in transplanted patients, when compared with healthy individuals (p<0.05). No statistically significant differences were observed regarding HHV-7 infection. Clinical symptoms and laboratorial findings were not specifically associated with patients shedding any of the studied viruses. Our results showed relevant differences in Roseolovirus prevalence among the two studied groups, suggesting a potential role for those viruses in disturbing host homeostasys that can compromise life quality. Although PCR methodology proved to be a useful tool for Roseolovirus detection, the standardization of samples and procedures is necessary to evaluate possible a pathogenic behavior among different agents in order to analyze their role in the post-transplant scenario.

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Section: Discussionsupporting

confidence: 92%

Section: Hhv6b and Hhv7mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Human Herpesviruses 6 and 7 Dna in the Saliva of Renal Transplanted Patients and Healthy Individuals From Rio De Janeiro, Brazil

Martins¹,

Cossatis²,

Afonso³

et al. 2011

VR&R

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“…The rate of oral excretion of HHV-6 in the healthy population is low (i.e. about 10%), while oral shedding rates of HHV-7 can vary from 12.6% to 90% depending on the population group [34,37]. HHV-6 and HHV-7 can be observed not only in healthy patients, but also in immunocompromised ones.…”

Section: Discussionmentioning

confidence: 99%

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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Background: Xerostomia is a very relevant and frequent complication of radiotherapy, causing the irradiated oral mucosa to be affected by bacterial, fungal and viral infections. Objective: The objective of this study was to evaluate a possible relationship between oral shedding of human herpesviruses and xerostomia in patients with squamous cell carcinoma of head and neck submitted to radio/chemotherapy. Methods: In this study, oral rinse samples were collected weekly from 20 patients during radiotherapy. The samples were submitted to PCR and enzymatic digestion for detection of human herpesviruses. Xerostomia was evaluated according to the Seminars in Radiation Oncology criteria. Results: There was a higher frequency of grade 1 xerostomia (51.4%), observed first in the 1st week of radiotherapy. In the 4th week of radiotherapy, all patients presented some degree of xerostomia. Analysis of herpesviruses showed oral shedding of EBV, HHV-6 and HHV-7 in all weeks. Considering all the periods, the highest frequency was in patients with EBV excretion (55.0%), which was significantly higher than that of other viruses. Conclusion: We observed that oral shedding of herpesviruses was not affected by xerostomia as there was a progression in their excretion, even with the evolution of xerostomia. This suggested that there is a local replication in the oral cavity that is not completely dependent of salivary excretion.

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“…O HHV-6 é o agente etiológico do exantema súbito, enquanto a infecção por HHV-7 é geralmente assintomática (Ward et al, 2005;Pinheiro et al, 2013). A taxa de excreção oral do HHV-6 na população geral é baixa, girando em torno de 10% (Biganzoli et al, 2010;Magalhães et al, 2010). As taxas de excreção oral de HHV-7 são bastante diferentes, variando de 12.6% a 90% (Biganzoli et al, 2010) dependendo da população estudada.…”

Section: Discussionunclassified

Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço

Palmieri¹

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Palmieri M. Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço. Dissertação apresentada à Faculdade de Odontologia da Universidade de São Paulo, para obter o título de Mestre, pelo Programa de Pós-Graduação em Ciências Odontológicas. A radioterapia para tratamento das neoplasias malignas em região de cabeça e pescoço é acompanhada de diversas complicações, decorrentes do comprometimento dos tecidos radiossensíveis localizados próximos ao tumor.Entre essas complicações a mucosite é a que merece maior destaque. A mucosite é uma reação tóxica inflamatória da mucosa oral causada pelo tratamento citorredutivo induzido pela radioterapia (RT) ou pela quimioterapia (QT). Ela manifesta-se com sinais de edema, eritema, úlcera e formação pseudomembrana, resultando em sintomas de ardência, que pode progredir para dor intensa e consequente prejuízo na alimentação e comunicação verbal.Infecções bacterianas, fúngicas ou virais podem acometer a mucosa bucal irradiada e exacerbar a manifestação da mucosite oral por meio da ativação de fatores de transcrição da resposta inflamatória. Existem poucos dados na literatura sobre a participação dos herpesvirus humanos na mucosite oral induzida pela radioterapia. A proposta desse trabalho foi avaliar a excreção oral dos herpesvirus humanos (HSV-1, HSV-2, EBV, CMV, VZV, HHV6, HHV7 e HHV8) e sua possível associação com o desenvolvimento e agravamento da mucosite oral, em pacientes diagnosticados com carcinoma epidermoide (CEC)de boca e orofaringe, submetidos à radioterapia associado à quimioterapia.Nesse estudo foram analisadas 158 amostras de lavado bucal, de 20 pacientes, submetidos à radioterapia para CEC em região de cabeça e pescoço, coletadas semanalmente, durante todo o tratamento. Foi realizada a extração do DNA dessas amostras e em seguida sua amplificação através da PCR utilizando dois conjuntos de primers: HSVP1/P2 para os subtipos HSV-1, HSV-2, EBV, CMV e HHV-8 e o VZVP1/P2 para os subtipos VZV, HHV-6 e HHV-7. As amostras positivas foram submetidas à digestão enzimática com enzimas de restrição BamHI e BstUI para determinação específica de cada um dos oito herpesvirus. Foi também avaliada clinicamente, a mucosite oral, em cada uma das coletas, seguindo os critérios da OMS e NCIC. As análises da amostra mostraram a excreção do EBV, HHV-6 e HHV-7, em todas as semanas de tratamento radioterápico, enquanto que a excreção do HSV1 não pode ser observada no momento da triagem. Considerando-se todos os períodos em conjunto (Triagem, semanas On our study, we analyzed 158 oral rinsing samples, collected weekly, from 20 patients during the whole radiotherapy treatment for squamous cell carcinoma in head and neck. From these samples, we extracted the DNA and afterwards we amplified them with PCR using two sets of primers:HSVP1/P2 for the subtypes HSV-1, HSV-2, EBV, CMV, and HHV-8 and VZVP1/P2 for the subtypes VZV, HHV 6, and HHV-7. The positive samples were subjected to enzymatic digestion with Ba...

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Detection of human herpesvirus 6 and 7 DNA in saliva from healthy adults from Rio de Janeiro, Brazil

Cited by 19 publications

References 24 publications

Detection of Human Herpesviruses 6 and 7 Dna in the Saliva of Renal Transplanted Patients and Healthy Individuals From Rio De Janeiro, Brazil

Detection of Human Herpesviruses 6 and 7 Dna in the Saliva of Renal Transplanted Patients and Healthy Individuals From Rio De Janeiro, Brazil

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço

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