No abstract

Merkel cell carcinoma (MCC) is a rare but aggressive neuroendocrine cancer, with approximately 80% of cases associated with Merkel cell polyomavirus (MCPyV). The lack of information concerning its occurrence in non-MCC immunosuppressed populations led to the investigation of MCPyV DNA in saliva and oral biopsies from 60 kidney allograft recipients and 75 non-transplanted individuals (control group). In contrast to herpesviruses, which was also investigated (CMV, HHV-6A, and B, HHV-7) MCPyV was detected predominantly in patients with oral lesions (gingivitis and/or periodontitis) of both transplanted and non-transplanted groups (P=0.016) and in the saliva of the transplanted group (P=0.009). MCPyV co-detection with CMV (P=0.048), and HHV-6 (P=0.020) in the saliva of transplanted patients requires further investigation on a possible role of co-infection.

In this study, we have sought to verify the prevalence ofhuman herpesviruses 6 and 7 (HHV-6, HHV-7) in the saliva of renal transplanted patients from Rio de Janeiro State, Brazil, and compare results with those of healthy subjects, since Roseolovirus DNA detection in body fluids from transplanted patients has been associated with often misdiagnosed chronic symptoms, organ rejection and even death. The studied group was composed by 120 individuals: 60 were renal transplanted patients and 60 were healthy subjects attending the Hospital Universitário Pedro Ernesto, for odontological follow-up. Saliva specimens were submitted to a multiplex nested polymerase chain reaction (PCR) to detect the presence of HHV-6A, HHV-6B and HHV-7. The total Roseolovirus DNA prevalence was 56.7% for transplanted patients and 23.3% for healthy individuals (p<0.001). For immunossupressed patients, the PCR detected a HHV-6A prevalence of 16.7% in transplanted, HHV-6B in 26.6% and HHV-7 DNA was revealed in 13.3% of the studied cases. In healthy subjects, HHV-6A was found in 5% of the samples, HHV-6B in 6.7% and HHV-7 in 11.7%. Multiple infections were observed in 12/60 (20%) individuals. No co-infection was demonstrated for healthy subjects, reinforcing the idea that imunnossuppression can favor reactivation and possibly transactivation among herpesviruses (P<0.001). Statistically significant differences were recorded for HHV6A and B infections in transplanted patients, when compared with healthy individuals (p<0.05). No statistically significant differences were observed regarding HHV-7 infection. Clinical symptoms and laboratorial findings were not specifically associated with patients shedding any of the studied viruses. Our results showed relevant differences in Roseolovirus prevalence among the two studied groups, suggesting a potential role for those viruses in disturbing host homeostasys that can compromise life quality. Although PCR methodology proved to be a useful tool for Roseolovirus detection, the standardization of samples and procedures is necessary to evaluate possible a pathogenic behavior among different agents in order to analyze their role in the post-transplant scenario.

A osseointegração é parte do processo que garante sucesso na implantodontia a partir de uma forma segura e eficiente de instalação do implante, tornando-o propício a receber carga proveniente da mastigação. Sabe-se que vários fatores estão envolvidos, como qualidade óssea planejamento, técnica correta, resposta cicatricial e prótese adequada. Como parte do processo da osseointegração, além da escolha do implante correto, o tratamento de superfície, que refere-se a um processo que modifica a camada mais superficial de um implante dentário com a finalidade otimizar o processo de osseointegração, diminuindo a possibilidade de problemas que possam levar à perda do implante. Este processo pode ser realizado de diversas formas, como relatado no presente artigo através da escrita e de mecanismos pedagógicos. Seu objetivo final é promover contato íntimo entre osso e implante, e a partir do que foi relatado neste trabalho pode-se verificar sua imprescindível atuação dentre os diversos tipos de tratamento possíveis. Foi possível constatar que o tratamento de superfície pode ser considerado peça chave no processo de ossointegração e que o planejamento correto irá direcionar qual é o melhor tratamento de superfície para cada caso.