Detection of gold-nanorod targeted pathogens using optical and piezoelectric optoacoustic sensors: comparative study

Conjusteau, André; Maswadi, Saher; Ermilov, Sergey A.; Brecht, Hans-Peter; Barsalou, Norman; Glickman, Randolph D.; Oraevsky, Alexander A.

doi:10.1117/12.813434

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Optical detectors utilizing interferometric techniques [43,[53][54][55], oblique-incidence reflectance [56], microring resonators [57], fiber-optic lines [58,59], and laser beam deflection [60,61] can also be applied to detect ultrawideband optoacoustic signals. In addition to ultrawideband sensitivity, there are three main advantages of optical detection: (1) possibility for remote (noncontact) optoacoustic detection; (2) small finite size of the virtual detectors enabled by the tightly focused laser beam, which in turn yields high lateral resolution; and (3) possibility of avoiding acoustic diffraction and wavefront distortion in the handheld probes operating in the backward mode [62].…”

Section: Optoacoustic Transducersmentioning

confidence: 99%

Near-Infrared Fluorescence Imaging and Spectroscopy in Random Media and Tissues

2014

Biomedical Photonics Handbook

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Section: Optoacoustic Transducersmentioning

confidence: 99%

Near-Infrared Fluorescence Imaging and Spectroscopy in Random Media and Tissues

2014

Biomedical Photonics Handbook

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“…We have previously reported on the development of an optoacoustic biosensor intended for the detection of bloodborne microorganisms using immunoaffinity reactions of antibody-coupled gold nanorods as contrast agents specifically targeted to the antigen of interest [30][31][32] . The sensitivity of Nano-LISA is at least OD=10 -6 which allows reliable detection of 1 pg/ml (depending on the commercial antibodies that are used).…”

Section: Nanolisa Prototypementioning

confidence: 99%

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

2012

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Gold nanorods (GNR) with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide (CTAB) on the surface of GNR with 16-mercaptohexadecanoic acid (MHDA) and metoxy-poly(ethylene glycol)-thiol (PEG), and the monoclonal antibodies: HER2 or CD33. The physical chemistry property of the conjugates was monitored through optical and zetapotential measurements to confirm surface chemistry. The plasmon resonance is kept in the near infrared area, and changes from strong positive charge for GNR-CTAB to slightly negative for GNR-PEG-mAb conjugates are observed. The conjugates were investigated for different cells lines: breast cancer cells and human leukemia lines in vivo applications. These results demonstrate successful tumor accumulation of our modified PEG-MHDA conjugates of GNR for HER2/neu in both overexpressed breast tumors in nude mice, and for thermolysis of human leukemia cells in vitro. The conjugates are non-toxic and can be used in pre-clinical applications, as well as molecular and optoacoustic imaging, and quantitative sensing of biological substrates.

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“…[9][10][11][12][13][14][15] Photoacoustic sensitivity can be greatly enhanced by the use of contrast agents such as metal nanoparticles, which have a strong photoacoustic response and have proven to be useful in both imaging and sensing applications. [16][17][18] The flexibility, speed, and sensitivity of photoacoustic technologies make them suitable for a variety of applications, including ocular imaging and sensing.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic Imaging and Sensing: A New Way to See the Eye

Glickman

2021

Journal of Ocular Pharmacology and Therapeutics

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Purpose: Photoacoustics (optoacoustics) is a hybrid technology utilizing light excitation of acoustic responses in targets of interest. It has found numerous applications in biomedicine, including eye research, because of its ability to report both morphological and functional data about the interrogated tissue. This presentation will give an overview of current applications. Methods: Wavelength-dependent absorption of light in a tissue chromophore causes local heating, leading to a thermoelastic expansion-contraction cycle. If nanosecond pulses of light are used to excite this process, the resulting pressure wave is an ultrasound signal propagating through the tissue and detectable at the tissue surface. This is highly advantageous because of the known properties of ultrasound propagation in tissue and the ability to use standard, medical ultrasound equipment for detection. The time of arrival and amplitude of ultrasound signals provide information about the location and nature of the absorber. Results: Due to the wavelength dependence of the photoacoustic response, functional and physiological applications are possible. For example, retinal oximetry can be determined from the different optical absorption properties of oxy-and deoxyhemoglobin. Multispectral imaging of the posterior segment can identify pigments such as melanin or lipofuscin or the nature of foreign bodies. The technique can be combined with other imaging modalities such as ultrasound and optical coherence tomography to produce high-resolution images of retinal structures along with functional information. Conclusion: Photoacoustic technology is a powerful noninvasive tool for ocular research and to study ocular morphology, fundamental physiological parameters, cellular responses, and molecular expression.

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Detection of gold-nanorod targeted pathogens using optical and piezoelectric optoacoustic sensors: comparative study

Cited by 7 publications

References 17 publications

Near-Infrared Fluorescence Imaging and Spectroscopy in Random Media and Tissues

Near-Infrared Fluorescence Imaging and Spectroscopy in Random Media and Tissues

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

Photoacoustic Imaging and Sensing: A New Way to See the Eye

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