Detection of glycopeptide resistance genes in enterococci by multiplex PCR

Bhatt, Puneet; Sahni, A.K.; Praharaj, Ashok Kumar; Grover, Naveen; Kumar, Manjit; Chaudhari, CN; Khajuria, Atul

doi:10.1016/j.mjafi.2014.03.005

Cited by 17 publications

(19 citation statements)

References 11 publications

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“…It spreads to patients in the hospital or is transmitted through the food supply. Enterococci are intrinsically resistant or tolerant to many antibiotics, and can acquire drug resistance either via integrons, plasmid transfer, or transposon acquisition [14–16].…”

Section: Introductionmentioning

confidence: 99%

“…The first-line treatment of choice for systemic enterococcal infections are glycopeptide antibiotics, mainly vancomycin (Van) and teicoplanin (Tei). Enterococcus faecalis and E. faecium are most frequently described as etiological agents of nosocomial infections caused by vancomycin-resistant enterococci (VRE) [14]. Until now, nine distinct vancomycin resistance gene clusters have been described in enterococci ( vanA , vanB , vanC , vanD , vanE , vanG , vanL , vanM , and vanN ) [17].…”

Section: Introductionmentioning

confidence: 99%

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Methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococci in fecal samples of birds from South-Eastern Poland

et al. 2019

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BackgroundThe incidence of human infection and colonization with methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) has increased in the recent years. Environmental sources, including bird droppings, might play an important role as resistance reservoirs.ResultsFresh fecal samples were collected from rooks and wild-living birds during the autumn-winter period of 2016/2017, and tested for the presence of bacteria associated with human diseases. Besides bacteria representing the genera Enterococcus, Campylobacter, Escherichia, and Staphylococcus, Enterobacter, Citrobacter, Proteus, Hafnia, and Pseudomonas were also identified. The susceptibility of S. aureus and Enterococcus spp. isolates to methicillin, and vancomycin and teicoplanin, respectively, was analyzed to assess the avian wildlife as a reservoir of MRSA and VRE strains. Twenty-two percent of all S. aureus isolates were methicillin-resistant. These strains were screened by polymerase chain reaction (PCR), using the most widely used primer sets specific for the mecA gene. Twenty percent of all Enterococcus strains were phenotypically vancomycin-resistant. The presence of van resistance genes in these strains was investigated by PCR using vanA and vanB gene-specific primers. A good correlation between mecA gene detection and disc diffusion data was observed, while some discrepancy was noted between the PCR data and the vancomycin/teicoplanin phenotypic resistance pattern. The incidence of strains resistant to methicillin and glycopeptide antibiotics in wild-living birds was twice that in rooks.ConclusionsThe study suggests that rooks from urban areas and passerine birds from the natural habitat carry antibiotic-resistant Enterococcus spp. and S. aureus strains, probably reflecting the presence of such isolates in the environmental food sources.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococci in fecal samples of birds from South-Eastern Poland

et al. 2019

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“…Different isolates of E. faecalis and E. faecium exhibited varied strategies of virulence factors.). In India Bhatt et al, (2014) established that VanA gene was observed in all the fourteen isolates by Multiplex PCR. One of the PCR amplicons was directed for sequencing and the sequence established was submitted in the GenBank (GenBank accession no.…”

Section: Enterococcus Virulence Genesmentioning

confidence: 99%

“…Amongst VRE colonized patients, (6, 4.5%) acquired VRE infection. Karimzadeh et al, (2016) Bhatt et al, (2014) demonstrated that 14, (14.6%) out of 96 Enterocoocus spp isolates, were resistant to vancomycin via vancomycin E test method (MIC32mg/ml).…”

Section: Vancomycin Resistant Enterococci (Vre)mentioning

confidence: 99%

History, Virulence Genes, Identification and Antimicrobial Resistance of Enterococcus faecalis Isolated from Diabetic Foot Patients

Bloushy¹,

Elbehiry²

2018

Int.J.Curr.Microbiol.App.Sci

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“…In addition to being the most relevant to hospital-acquired infections, E. faecalis and E. faecium are also the two main reservoirs for vanA, vanB, and vanM, which are the genes contributing to high levels of resistance to vancomycin; therefore, they are the main targets of studies focused on VRE. Vancomycin-resistant E. faecalis has been more frequently detected than other Enterococcus species; however, E. faecium is recently being increasingly detected [16,17].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci

et al. 2020

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The prevalence of vancomycin resistant enterococcus (VRE) carrier-state has been increasing in patients of intensive care unit and it would be a public health threat. Different research groups conducted decolonizing VRE with probiotic and the results were controversial. Therefore, a systemic approach to search for the probiotic species capable of decolonizing VRE is necessary. Thus, VRE was co-cultured with ten probiotic species. The fluctuations of each bacterial population were analyzed by 16S rRNA sequencing. Microbial network analysis (MNA) was exploited to identify the most critical species in inhibiting the VRE population. The MNA-selected probiotic cocktail was then validated for its efficacy in inhibiting VRE, decolonizing VRE from Caco-2 cells via three approaches: exclusion, competition, and displacement. Finally, the expression of VRE virulence genes after co-incubation with the probiotic cocktail were analyzed with quantitative real-time PCR (qRT-PCR). The MNA-selected probiotic cocktail includes Bacillus coagulans, Lactobacillus rhamnosus GG, Lactobacillus reuteri, and Lactobacillus acidophilus. This probiotic combination significantly reduces the population of co-cultured VRE and prevents VRE from binding to Caco-2 cells by down-regulating several host-adhesion genes of VRE. Our results suggested the potential of this four-strain probiotic cocktail in clinical application for the decolonization of VRE in human gut.

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Detection of glycopeptide resistance genes in enterococci by multiplex PCR

Cited by 17 publications

References 11 publications

Methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococci in fecal samples of birds from South-Eastern Poland

Methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococci in fecal samples of birds from South-Eastern Poland

History, Virulence Genes, Identification and Antimicrobial Resistance of Enterococcus faecalis Isolated from Diabetic Foot Patients

Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci

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