Detection of Extended-Spectrum-Beta-Lactamase (ESBL) Producing Escherichia Coli in Meat Chicken from Traditional Market in Surabaya, East Java, Indonesia

Wardhana, Dhandy Koesoemo; Effendi, Mustofa Helmi; Harijani, Nenny; Ooi, Hong-Kean

doi:10.37506/v11/i1/2020/ijphrd/194032

Cited by 7 publications

(5 citation statements)

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“…Also, reports from local markets in Indonesia recorded huge microbial contamination of several chicken meats by Salmonella spp. (85 %) and E. coli (90.03 %) [57,58]. Findings from this study further corroborated our study on the prevalence of S. aureus, Salmonella spp., and E. coli in chicken meat.…”

Section: Microbiological Analysis Of Chicken Samplessupporting

confidence: 91%

Chemical and microbiological quality of commercial fresh and frozen chicken drumstick in Umuhia, Nigeria

2022

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Chicken meat, an excellent source of good quality proteins, is highly susceptible to microbial contaminations and heavy metals from feeds, processing, and retailing environments, thereby posing health risks to consumers. Therefore, the proximate composition, heavy metal concentrations, and microbial loads of commercial fresh and frozen chicken drumsticks, randomly sourced from poultry farms and groceries, respectively, within Umuahia metropolis, Abia State, Nigeria, were investigated to ascertain their qualities. A total of 40 (20 each of the fresh and frozen) chicken drumstick samples were processed and analyzed for proximate (moisture, fats, ash, proteins, fibers, and carbohydrates), heavy metal (cadmium, chromium, copper, manganese, nickel, zinc, and lead) contents, and microbial loads (total viable counts, Escherichia coli counts, Salmonella counts, Staphylococcus counts and fungal counts) and characterization using standard techniques. Results were analyzed statistically (p<0.01, 0.05). The fresh chicken drumstick samples had significantly higher (p<0.01, 0.05) levels of moisture (61.38 ± 0.34 %), proteins (22.30 ± 0.02 %), ash (5.27 ± 0.17 %) and microbial contents (0.24 × 102 CFU/g to 2.42 × 104 CFU/g), when compared with the frozen drumstick samples with significantly higher (p<0.01, 0.05) contents of fats (3.70 ± 0.13 %), carbohydrates (12.55 ± 0.27 %) and heavy metals (0.047 ± 0.017 mg/kg to 0.852 ± 0.456 mg/kg). This suggests that fresh chicken drumstick is more nutritionally beneficial, provided they are thoroughly treated with heat before consumption to prevent foodborne diseases from bacterial and fungal contaminations. Although the levels of heavy metals in both meat samples are below the toxic limits, consumers should be wary of bioaccumulation and bio-magnification of heavy metals when constantly exposed to them. Hence, constant surveillance of chicken meat is encouraged to monitor their exposure levels to these chemicals and biohazards.

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Section: Microbiological Analysis Of Chicken Samplessupporting

confidence: 91%

Chemical and microbiological quality of commercial fresh and frozen chicken drumstick in Umuhia, Nigeria

2022

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“…Widhi and Saputra (2021) reported a of 71.4% in a sample from the Purwokerto City Market. Wardhana et al (2020) reported a prevalence of 90.03% in a sample from the Surabaya Traditional Market.…”

Section: Discussionmentioning

confidence: 98%

Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia

YANESTRIA

Dameanti²,

MUSAYANNAH³

et al. 2022

Biodiversitas

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Abstract. Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi MH, Ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas 23: 4460-4465. Escherichia coli is one of the bacteria that can be used as an indicator of environmental pollution. This bacterium has the ability to become an antimicrobial-resistant bacterium that impacts public health. The antimicrobial ability can develop into a bacterium that produces extended-spectrum ?-lactamase (ESBL). The purpose of this study was to reveal the occurrence of extended-spectrum ?-lactamase (ESBL) producing E. coli from a broiler farm environment in Pasuruan district and to describe the phenotypic pattern of E. coli producing ESBL that has been detected. A total of 175 samples were used in this study consisting of 115 samples of coop swabs and 65 samples of wastewater around the farm. The samples were isolated and identified to find E. coli by using different culture media viz. McConkey agar (MCA), eosin methylene blue agar (EMBA), Gram staining, indole test, methyl red Voges Proskauer (MR-VP), citrate, and triple sugar iron agar (TSIA). Detection of ESBL using the double disc synergy test (DDST) according to standard Clinical and Laboratory Standards Institute procedures and the VITEK®2 compact apparatus. The results of ESBL confirmation with DDST and VITEK®2 showed that 16 (9.14%) of the 175 environmental samples confirmed E. coli produced ESBL. The results of the VITEK®2 test also produced a phenotypic pattern of resistance properties of ESBL-producing E. coli and found 12 types of resistance patterns. The combination of “AM AMP ATM KZ CTX CRO CIP” and “AM AMP ATM KZ CTX CRO GM CIP SXT” are the 2 most common resistance patterns (18.75%), while the other 10 resistance patterns occur at the same level (6.25%). The data presented here confirmed the presence of ESBL-producing E. coli in the farm environment, which can contribute to the dissemination of MDR bacteria in the environment if not monitored. Therefore, the presence of ESBL-producing E. coli in Pasuruan is worrisome since it can lead to an impact on human health.

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“…The previous studies also stated that several chicken meat samples from local markets in Indonesia presented microbial contamination, which included S. aureus (6.7%) [11], Salmonella spp. (85%) [12], and E. coli (90.03%) [13]. The contamination of poultry products including raw broiler meat by pathogenic microorganisms, especially bacteria, has become one of the most challenging problems in the food industry worldwide [14].…”

Section: Introductionmentioning

confidence: 99%

Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia

et al. 2021

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Background and Aim: Chicken meat can be contaminated by microorganisms anywhere in the supply chain, from farm to market, and these microorganisms can be transmitted to humans through direct contact, contact with the environment, and food consumption. The microbial contamination has a serious impact on public health. This study aimed to analyze the microbial contamination of chicken meat sampled from local markets in Surabaya, East Java, Indonesia. Materials and Methods: A total of 60 samples of fresh chicken meat obtained from 10 traditional markets (six samples per market) were examined for the presence of bacteria. Staphylococcus aureus, Salmonella spp., and Escherichia coli were identified using Gram staining, culturing, and biochemical tests. The most probable number (MPN) method was used to identify E. coli. Results: Most chicken meat samples were positive for S. aureus (58.3%), Salmonella spp. (48.3%), and E. coli (40%). The samples were considered positive for E. coli if the MPN value was higher than 1×101 CFU/g. Conclusion: High microbial contamination was found in all the chicken meat sampled from local markets in Surabaya. Such contamination can lead to foodborne diseases so, proper hygiene and sanitation standards should be followed from slaughterhouses to the end-users.

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Detection of Extended-Spectrum-Beta-Lactamase (ESBL) Producing Escherichia Coli in Meat Chicken from Traditional Market in Surabaya, East Java, Indonesia

Cited by 7 publications

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Chemical and microbiological quality of commercial fresh and frozen chicken drumstick in Umuhia, Nigeria

Chemical and microbiological quality of commercial fresh and frozen chicken drumstick in Umuhia, Nigeria

Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia

Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia

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