Detection of ESBL and AmpC producing Klebsiella pneumoniae ST11 and ST147 from urinary tract infections in Iran

Abstract

In the present study a total of 200 Klebsiella pneumoniae isolates were collected from patients with urinary tract infections (UTIs) in Tehran, Iran. Antibiotic resistance was determined by disk diffusion and broth dilution methods. Detection of extended-spectrum β-lactamases (ESBLs) and AmpCs was performed using phenotypic tests. Polymerase chain reaction (PCR) was applied to detect the ESBL, AmpC, and integron genes. Analysis of AmpC and cassette arrays of integron genes was performed using DNA sequencing. Plasmids were analyzed by PCR-based replicon typing and conjugation. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were applied to explore the genomic relatedness among the isolates. The highest levels of resistance were observed against ampicillin (100%), followed by piperacillin (57.5%), ceftazidime (46%), trimethoprim/sulfamethoxazole (44%), ciprofloxacin (32.5%), and imipenem (19%). Approximately, 66.5% of isolates harbored at least one of the beta-lactamase genes (bla TEM, bla SHV, bla CTX-M, and bla OXA-1). In addition, 22.5% of isolates carried at least one of the AmpC genes including bla DHA and bla CIT. Integron class I was the most prevalent integron among resistant isolates. According to the results of replicon typing, IncFII, IncL/M, and IncA/C were the most frequent replicons, respectively. All selected isolates were able to transfer bla CTX-M, also two isolates transferred the bla DHA-1 gene to Escherichia coli K12 through conjugation. Finally, 21 isolates were categorized into 4 pulsotypes and 11 unique clusters in PFGE. MLST identified ST147 and ST11 sequence types but ST147 was the most prevalent in the current study.

High Prevalence of blaOXA-48 and blaNDM-Producing Carbapenem-Resistant Klebsiella pneumoniae Isolated from Clinical Samples in Shahid Rajaei Hospital in Tehran, Iran

“…Various transferable plasmids harbor the bla NDM-1 and bla OXA-48 genes (IncA/C, IncFII, IncN, or untypeable plasmids, and IncL/M, IncN, IncA/C, or untypeable plasmids, respectively) (13,14). NDM-1 and OXA-48 carbapenemaseproducing K. pneumoniae (CPKP) show a remarkable dissemination ability.…”

Investigating the effects of zinc oxide and titanium dioxide nanoparticles on the formation of biofilm and persister cells in Klebsiella pneumoniae

“…Klebsiella pneumoniae is a gram-negative bacterium belonging to the Enterobacteriaceae family, which as an opportunistic human pathogen causes severe infections such as urinary tract infections, meningitis, pneumonia, and septicemia, especially in hospitalized patients [1,2]. Recent reports indicate the high prevalence of multidrug resistance in K. pneumoniae.…”

Evaluation of biosynthesized silver nanoparticles effects on expression levels of virulence and biofilm‐related genes of multidrug‐resistantKlebsiella pneumoniaeisolates

“…Treatment of severe healthcare-associated infections caused by this microorganism is complicated by antibiotic resistance. Antibiotic resistance among pathogenic bacteria is an issue that has been considered a global problem [5,6]. K. pneumoniae infections have long been treated with betalactam antibiotics.…”