High Prevalence of blaOXA-48 and blaNDM-Producing Carbapenem-Resistant Klebsiella pneumoniae Isolated from Clinical Samples in Shahid Rajaei Hospital in Tehran, Iran

Mokhtari, Maryam; Mojtahedi, Ali; Mahdieh, Nejat; Jafari, Alireza; Arya, Mohammad Javad

doi:10.5812/jjm-130804

Cited by 5 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OXA-48-like was found as the most prevalent carbapenemase being identified in all CRKP isolates followed by NDM and VIM enzymes. This is in accordance with recent studies which reported OXA-48-Like and NDM as the most frequent carbapenemases among CRKP isolates in Iran ( 20 , 21 ). Since the first recognition of OXA-48 enzyme in 2001, several variants of OXA-48 carbapenemases have emerged, collectively forming the “OXA-48-like” subfamily including OXA-181, OXA-232, OXA-204, OXA-162, OXA-163, OXA-244, OXA-245, OXA-370 and OXA-405.…”

Section: Discussionsupporting

confidence: 93%

“…However, 66.6% and 79% of CRKP isolates were identified as colistin and amikacin resistant respectively. While similar studies from Iran have reported tigecycline as the most effective antibiotic against CRKP with resistance rate of 3.3–3.9% ( 20 , 21 ), the identified resistance rates for amikacin in these studies were found to be 50.9% ( 20 ) and 38% ( 21 ). There have been increasing numbers of reports on colistin resistance among CRKP isolates worldwide ( 31 , 32 ), particularly from Iran ( 30 ) making this old agent less effective for treatment of CRE infections in the country.…”

Section: Discussionmentioning

confidence: 57%

See 1 more Smart Citation

High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant Klebsiella pneumoniae of clinical origin from Iran

Ghanbarinasab,

Haeili,

Ghanati

et al. 2023

IJM

View full text Add to dashboard Cite

Background and Objectives: Klebsiella pneumoniae is increasingly developing resistance to last-resort antibiotics such as carbapenems. This study aimed to investigate the dissemination of common carbapenemase encoding genes among 48 clinical isolates of carbapenem-resistant Klebsiella pneumoniae (CRKP). Materials and Methods: Antimicrobial susceptibility testing was performed by broth dilution and disc diffusion methods. The phenotypic evaluation of carbapenemase production was performed by using Modified Carbapenem Inactivation Meth- od. Presence of carbapenemase encoding genes bla KPC , bla , bla OXA-48-like , bla , and bla VIM was screened by PCR.R. Results: Overall, carbapenemases were produced in all CRKP isolates. The bla OXA-48-like and bla NDM were the most prevalent genes detected among all and 66.6% (n=32) of CRKP isolates respectively. The bla VIM was detected in only one isolate co-harboring NDM and OXA-48-like carbapenemases. The bla KPC and bla IMP genes were not identified in any of the isolates. While tigecycline was the most active agent against CRKP isolates with low resistance rate (4.1%), high rate of resistance was observed to colistin (66.6%), amikacin (79%) and most of other tested antimicrobials. Conclusion: Our results revealed predominant prevalence of OXA-48-like and NDM carbapenemases among CRKP clinical isolates. High rate of resistance to last-resort agents such as colistin among CRKP isolates is a source of great concern.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 57%

High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant Klebsiella pneumoniae of clinical origin from Iran

Ghanbarinasab,

Haeili,

Ghanati

et al. 2023

IJM

View full text Add to dashboard Cite

show abstract

“…A systematic review and meta-analysis on the prevalence of colistin resistance of K. pneumoniae isolates in Iran revealed that the pooled prevalence of colistin resistance in clinical isolates was 6.9% 10 . However, the rate of K. pneumoniae carbapenem resistance was more than 73% in different studies 11 , 12 .…”

Section: Introductionmentioning

confidence: 96%

Prevalence and epidemiological investigation of mgrB-dependent colistin resistance in extensively drug resistant Klebsiella pneumoniae in Iran

Zahedi Bialvaei,

Eslami,

Ganji

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Carbapenemases-producing K. pneumoniae are challenging antimicrobial therapy of hospitalised patients, which is further complicated by colistin resistance. The aim of this study was to investigate the molecular epidemiological insights into carbapenemases-producing and colistin-resistant clinical K. pneumoniaeA total of 162 colistin resistant clinical strains of K. pneumoniae were collected during 2017–2019. Antimicrobial susceptibility and the colistin minimum inhibitory concentration were determined. Using PCR assay, the prevalence of resistance-associated genes including blaKPC, blaIMP, blaVIM, blaOXA-48, blaNDM-1 and mcr-1 to -9 was examined. Additionally, a PCR assay was used to examine the mgrB gene in colistin-resistant bacteria. 94.4% of the tested strains were resistant to imipenem and 96.3% were resistant to meropenem. Colistin resistance (MIC > 4 µg/L) was observed in 161 isolates (99.4%) by Colistin Broth Disk Elution method. The KPC enzyme was the most common carbapenemase and was identified in 95 strains (58.6%), followed by the IMP, VIM and OXA-48 detected in 47 (29%), 23 (14.2%) and 12 (7.4%) isolates, respectively. However, no NDM-1 gene was detected. Additionally, none of the studied isolates harbored mcr variants, while mgrB gene was observed in 152 (92.6%) isolates. Colistin resistance of K. pneumoniae isolates may be associated with mgrB gene mutation. To stop the spread of resistant K. pneumoniae, surveillance must be improved, infection prevention protocols must be followed, and antibiotic stewardship must be practised.

show abstract

“…The isolates carry higher variants of OXA exhibit a high level of resistance to β-lactams, monobactams, and carbapenems as well as to aminoglycosides and fluoroquinolones [ 5 ]. Due to the increase of carbapenem-resistant E. coli in different regions of the world, periodic epidemiological surveillance and investigating the mechanisms of carbapenem-resistant isolates are essential for the global control of CRE [ 7 ]. The importance of the horizontal transfer of carbapenemase genes through mobile genetic elements and its relationship with increasing resistance to different groups of antibiotics has been highlighted [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

The emergence of plasmid-encoded oxacillinase and carbapenemase among uropathogenic Escherichia coli (UPEC) isolated from hospitalized patients in the North of Iran

Sadeghi¹,

Mojtahedi²,

Nikokar³

et al. 2023

Heliyon

Self Cite

View full text Add to dashboard Cite

High Prevalence of blaOXA-48 and blaNDM-Producing Carbapenem-Resistant Klebsiella pneumoniae Isolated from Clinical Samples in Shahid Rajaei Hospital in Tehran, Iran

Cited by 5 publications

References 42 publications

High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant Klebsiella pneumoniae of clinical origin from Iran

High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant Klebsiella pneumoniae of clinical origin from Iran

Prevalence and epidemiological investigation of mgrB-dependent colistin resistance in extensively drug resistant Klebsiella pneumoniae in Iran

The emergence of plasmid-encoded oxacillinase and carbapenemase among uropathogenic Escherichia coli (UPEC) isolated from hospitalized patients in the North of Iran

Contact Info

Product

Resources

About