Evaluation of biosynthesized silver nanoparticles effects on expression levels of virulence and biofilm‐related genes of multidrug‐resistant <i>Klebsiella pneumoniae</i> isolates

Mousavi, Seyed Mohammad; Moeinizadeh, Mostafa; Aghajanidelavar, Mahshad; Rajabi, Sajad; Mirshekar, Maryam

doi:10.1002/jobm.202200612

Cited by 9 publications

(3 citation statements)

References 47 publications

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“…The suppression of bio lm formation may be the result of SFELS-AgNPs penetrating the bacterial cell wall, suppressing the synthesis of exopolysaccharide layers, and interfering with cell adhesion and communication [49]. Mousavi et al, recently studied bio lm reduction in K. pneumonia strains using biologically synthesized silver nanoparticles their results are comparable with this study as they have obtained signi cant reduction at 128 µg/mL of nanoparticles [50]. Additionally, prior research indicated that these NPs might be effective antibacterial agents against a variety of bacterial infections, such as K. pneumoniae [51].…”

Section: Effect Of Sfels-agnps On K Pneumonia Bio Lm Formationsupporting

confidence: 71%

Biosynthesis of silver nanoparticles using supercritical CO 2 mediated phenolic contents extracted from Lagerstroemia speciosa leaf inhibits Klebsiella pneumoniae biofilm formation

Khandare,

Kumar,

Sharma

et al. 2024

Preprint

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A large number of scientists are now working in order to create silver nanoparticles (AgNPs) that can be used as biomedicines against cancerous cell lines and bacteria that are resistant to drugs. In the current study, optimal supercritical fluid extract (SFE) of Lagerstroemia speciosa (LS) leaves at pressure 29.59 MPa, temperature 89.50 ºC and extraction time 53.85 min. was used to extract phenolic compounds for the synthesis of AgNPs. The synthesis was studied for 0–20 hrs. Initially the synthesis was confirmed by observing change in colour phenomenon. UV -spectroscopy confirmed the synthesis of nanoparticles (SFELS-AgNPs) demonstrated a maximum surface plasmon resonance at 430 nm. The crystallite dimension of nanoparticles was determined using XRD (13.47 nm), TEM results confirmed the diameter of the obtained silver nanoparticles between 8–20 nm. The nanoparticles possessed − 25.6 mV electric charge on the surface confirmed using zeta potential analyser. Furthermore, energy-dispersive X-ray analysis (EDAX), was used to analyze the presence of differential elements in generated materials. The developed nanoparticles were evaluated for their potential antimicrobial properties against, two gram-positive viz. Staphylococcus aureus and Bacillus cereus, and three gram-negative bacteria viz. Klebsiella pneumonia, Pseudomonas aeruginosa and Escherichia coli with different concentrations (100–400 µg/mL). The nanoparticle showed a minimum inhibitory concentration (MIC) of 64 µg/ml whereas the minimum bactericidal concentration (MBC) 128 µg/ml against K. pneumonia. They significantly inhibited K. pneumonia biofilm formation confirmed using scanning electron microscopy (SEM). The results were encouraging compared to the standards drug Chloramphenicol and other controls. The generated nanoparticles have highly effective antimicrobial properties against pathogenic bacteria.

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Section: Effect Of Sfels-agnps On K Pneumonia Bio Lm Formationsupporting

confidence: 71%

Biosynthesis of silver nanoparticles using supercritical CO 2 mediated phenolic contents extracted from Lagerstroemia speciosa leaf inhibits Klebsiella pneumoniae biofilm formation

Khandare,

Kumar,

Sharma

et al. 2024

Preprint

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“…One study found that in resistant Klebsiella pneumoniae strains, the expression of the efflux pump gene OxqAB was decreased by both commercial and biosynthesized Ag NPs (Dolatabadi et al, 2021). What is more, Ag NPs were potential NPs that showed excellent reduction of biofilm-related genes (fimH, rmpA, and mrkA) in K. pneumoniae (Mousavi et al, 2023). Wang et al, developed the Ag NP-functionalized titanium implant surface.…”

Section: Ag Npsmentioning

confidence: 99%

Targeting bacterial biofilm-related genes with nanoparticle-based strategies

Afrasiabi,

Partoazar

2024

Front. Microbiol.

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Persistent infection caused by biofilm is an urgent in medicine that should be tackled by new alternative strategies. Low efficiency of classical treatments and antibiotic resistance are the main concerns of the persistent infection due to biofilm formation which increases the risk of morbidity and mortality. The gene expression patterns in biofilm cells differed from those in planktonic cells. One of the promising approaches against biofilms is nanoparticle (NP)-based therapy in which NPs with multiple mechanisms hinder the resistance of bacterial cells in planktonic or biofilm forms. For instance, NPs such as silver (Ag), zinc oxide (ZnO), titanium dioxide (TiO2), copper oxide (Cu), and iron oxide (Fe3O4) through the different strategies interfere with gene expression of bacteria associated with biofilm. The NPs can penetrate into the biofilm structure and affect the expression of efflux pump, quorum-sensing, and adhesion-related genes, which lead to inhibit the biofilm formation or development. Therefore, understanding and targeting of the genes and molecular basis of bacterial biofilm by NPs point to therapeutic targets that make possible control of biofilm infections. In parallel, the possible impact of NPs on the environment and their cytotoxicity should be avoided through controlled exposure and safety assessments. This study focuses on the biofilm-related genes that are potential targets for the inhibition of bacterial biofilms with highly effective NPs, especially metal or metal oxide NPs.

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“…Other properties of nanoparticles identified as responsible for antibiofilm roles are given by shape, surface charge, and composition. The adherence of bacteria is inhibited by using surfaces with nano-roughness [28,29,59].…”

Section: Introductionmentioning

confidence: 99%

TiO2 Nanocomposite Coatings and Inactivation of Carbapenemase-Producing Klebsiella Pneumoniae Biofilm—Opportunities and Challenges

Bereanu,

Vintilă,

Bereanu

et al. 2024

Microorganisms

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The worldwide increase of multidrug-resistant Gram-negative bacteria is a global threat. The emergence and global spread of Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella pneumoniae represent a particular concern. This pathogen has increased resistance and abilities to persist in human reservoirs, in hospital environments, on medical devices, and to generate biofilms. Mortality related to this microorganism is high among immunosuppressed oncological patients and those with multiple hospitalizations and an extended stay in intensive care. There is a severe threat posed by the ability of biofilms to grow and resist antibiotics. Various nanotechnology-based strategies have been studied and developed to prevent and combat serious health problems caused by biofilm infections. The aim of this review was to evaluate the implications of nanotechnology in eradicating biofilms with KPC-producing Klebsiella pneumoniae, one of the bacteria most frequently associated with nosocomial infections in intensive care units, including in our department, and to highlight studies presenting the potential applicability of TiO2 nanocomposite materials in hospital practice. We also described the frequency of the presence of bacterial biofilms on medical surfaces, devices, and equipment. TiO2 nanocomposite coatings are one of the best long-term options for antimicrobial efficacy due to their biocompatibility, stability, corrosion resistance, and low cost; they find their applicability in hospital practice due to their critical antimicrobial role for surfaces and orthopedic and dental implants. The International Agency for Research on Cancer has recently classified titanium dioxide nanoparticles (TiO2 NPs) as possibly carcinogenic. Currently, there is an interest in the ecological, non-toxic synthesis of TiO2 nanoparticles via biological methods. Biogenic, non-toxic nanoparticles have remarkable properties due to their biocompatibility, stability, and size. Few studies have mentioned the use of nanoparticle-coated surfaces as antibiofilm agents. A literature review was performed to identify publications related to KPC-producing Klebsiella pneumoniae biofilms and antimicrobial TiO2 photocatalytic nanocomposite coatings. There are few reviews on the antibacterial and antibiofilm applications of TiO2 photocatalytic nanocomposite coatings. TiO2 nanoparticles demonstrated marked antibiofilm activity, but being nano in size, these nanoparticles can penetrate cell membranes and may initiate cellular toxicity and genotoxicity. Biogenic TiO2 nanoparticles obtained via green, ecological technology have less applicability but are actively investigated.

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Evaluation of biosynthesized silver nanoparticles effects on expression levels of virulence and biofilm‐related genes of multidrug‐resistant Klebsiella pneumoniae isolates

Cited by 9 publications

References 47 publications

Biosynthesis of silver nanoparticles using supercritical CO 2 mediated phenolic contents extracted from Lagerstroemia speciosa leaf inhibits Klebsiella pneumoniae biofilm formation

Biosynthesis of silver nanoparticles using supercritical CO 2 mediated phenolic contents extracted from Lagerstroemia speciosa leaf inhibits Klebsiella pneumoniae biofilm formation

Targeting bacterial biofilm-related genes with nanoparticle-based strategies

TiO2 Nanocomposite Coatings and Inactivation of Carbapenemase-Producing Klebsiella Pneumoniae Biofilm—Opportunities and Challenges

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