Detection of conjugated diene isomers of linoleic acid in liver lipids of rats fed a choline-devoid diet indicates that the diet does not cause lipoperoxidation

Banni, S.; Day, Billy W.; Evans, Rhobert W.; Corongiu, Francesco P.; Lombardi, Benito

doi:10.1016/0955-2863(95)00049-6

Cited by 78 publications

(54 citation statements)

References 22 publications

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“…However, its use in the direct analysis of plasma is controversial because of the presence of interfering substances, such as heme proteins, purines or pyrimidines in the UV region measurement [42,57]. Increased sensitivity of the method can be achieved by an extraction of lipids into organic solvents in combination HPLC with UV detection [34,58]. However, the result of application the method to lipid extracts from human body fluids after HPLC separation was surprising, because the majority of pre-treated lipid fraction absorbs at wavelengths typical for conjugated dienes consisting of conjugated linoleic acid isomer (cis-9, trans-11-octadecadienoic acid) [59].…”

Section: Conjugated Dienesmentioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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Section: Conjugated Dienesmentioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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“…The presence of these derivatives has been mentioned in the liver of different species such as the rat [16][17][18]21], lamb [19] and mouse [20]. Moreover, it has been recently demonstrated in the rat that the liver is able to convert rumenic acid into a conjugated derivative identified on the basis of its retention time as the cis-6, cis-9, trans-11 C18:3 [21].…”

Section: Discussionmentioning

confidence: 99%

“…In such situations, large amounts of FA, including CLA which are stored in adipose tissues, are released into the blood and taken up by the liver. The intensity of the different CLA metabolic pathways in the liver is still poorly documented except for the fact that in the liver of different species such as the rat [16][17][18], lamb [19] and mouse [20] given CLA-supplemented diets, CLA is converted into conjugated C18:3 by ∆6 desaturase activity [2,17]. Although these studies reported the presence of CLA derivatives in the liver of animals, only Gruffat et al [21] recently demonstrated, by using rat liver slices incubated with cis-9, trans-11 and trans-10, cis-12 CLA isomers, that CLA conversion into conjugated C18:3 actually occurs in this organ.…”

Section: Introductionmentioning

confidence: 99%

In vitro metabolism of rumenic acid in bovine liver slices

et al. 2005

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-Ruminant products are the major source of CLA for humans. However, during periods of fat mobilization, the liver might play an important role in CLA metabolism which would limit the availability of the latter for muscles and milk. In this context, rumenic acid (cis-9, trans-11 CLA) metabolism in the bovine liver (n = 5) was compared to that of oleic acid (n = 3) by using the in vitro liver slice method. Liver slices were incubated for 17 h in a medium containing 0.75 mM of FA mixture and 55 µM of either [1-14 C] rumenic acid or [1-14 C] oleic acid at 37 °C under an atmosphere of 95% O 2 -5% CO 2 . Rumenic acid uptake by liver slices was twice (P = 0.009) that of oleic acid. Hepatic oxidation of both FA (> 50% of incorporated FA) led essentially to the production of acidsoluble products and to a lower extent to CO 2 production. Rumenic acid was partly converted (> 12% of incorporated rumenic acid) into conjugated C18:3. CLA and its conjugated derivatives were mainly esterified into polar lipids (71.7%), whereas oleic acid was preferentially esterified into neutral lipids (59.8%). Rumenic acid secretion as part of VLDL particles was very low and was onefourth lower than that of oleic acid. In conclusion, rumenic acid was highly metabolized by bovine hepatocytes, especially by the oxidation pathway and by its conversion into conjugated C18:3 for which the biological properties need to be elucidated. rumenic acid / oleic acid / metabolism / liver / bovine Abbreviations: ASP, acid-soluble products; BSA, bovine serum albumin; CLA, conjugated linoleic acid; FA, fatty acids; NL, neutral lipids; PL, polar lipids; VLDL, very-low density lipoproteins.

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“…According to Banni et al (1995), Menendez et al (2005), among the monounsaturated fatty acids, oleic acid was the main one, which has as main effects, reducing the fraction of total cholesterol and cholesterol low density lipoprotein (LDLc) without reducing the fraction of high lipoprotein cholesterol density (HDLc). Furthermore, it causes changes in the platelet membrane producing an anti-thrombotic action Kobori and Jorge (2005) analyzing the orange seed oil extracted by hot solvent, found peroxide value of 29.4 meq/ kg.…”

Section: Resultsmentioning

confidence: 99%

Physico-chemical Characterization of Seed Oils Extracted from Oranges (Citrus sinensis)

Aranha

Jorge

2013

FSTR

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This study aimed to characterize the seeds of oranges, varieties Hamlin, Natal, Pera-rio and Valencia, on the composition and physico-chemical properties of its oil through the determination of free fatty acids, peroxide value, refraction, iodine value, saponification value, unsaponifiable matter, oxidative stability and fatty acid profile. The results were submitted to analysis of variance and differences between the average results were tested at 5% probability by Tukey test. The orange seed oils showed high levels of lipids, about 40%, and showed a low degree of degradation, when analyzing the free fatty acids and peroxide value. It was found through the fatty acid profile and refraction and iodine indexes seed oil, Pera-rio orange was the most unsaturated. The seed oil, Valencia orange had a higher oxidative stability in Rancimat at 100℃.

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Detection of conjugated diene isomers of linoleic acid in liver lipids of rats fed a choline-devoid diet indicates that the diet does not cause lipoperoxidation

Cited by 78 publications

References 22 publications

Automation of Methods for Determination of Lipid Peroxidation

Automation of Methods for Determination of Lipid Peroxidation

In vitro metabolism of rumenic acid in bovine liver slices

Physico-chemical Characterization of Seed Oils Extracted from Oranges (Citrus sinensis)

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