Automation of Methods for Determination of Lipid Peroxidation

Sochor, Jiří­; Ruttkay-Nedecký, Branislav; Babula, Petr; Adam, Vojtěch; Hubálek, Jaromír; Kízek, René

doi:10.5772/45945

Cited by 33 publications

(39 citation statements)

References 99 publications

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“…The quantity of MDA was determined by measuring absorbance of MDA/TBA adduct (Sochor et al 2012). Samples were added to 0.25 % TBA in 10 % trichloroacetic acid.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Aspects of Reaction of Ozone with Galactolipid and Galactolipid–Tocopherol Layers

2014

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The impact of reaction of galactolipids with ozone on the physicochemical properties of their monolayers was examined. In Megli and Russo (Biochim Biophys Acta, 1778:143–152, 2008), Cwiklik and Jungwirth (Chem Phys Lett, 486:99–103, 2010), Jurkiewicz et al. (Biochim Biophys Acta, 1818:2388–2402, 2012), Khabiri et al. (Chem Phys Lett, 519:93–99, 2012), and Conte et al. (Biochim Biophys Acta, 1828:510–517, 2013), the properties of layers formed from model mixtures composed of chosen lipids and selected oxidation products were studied, whereas in this work, question was raised as to how the oxidation reactions taking place in situ affect the physical properties of the galactolipid layers. So, set experiment should take into account the effect of all reaction products. The mechanical characteristics of monolayers of monogalactosyldiacyl-glycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were determined by Langmuir trough technique, and the electrical properties of liposomes formed from these lipids by measuring their electrophoretic mobility. Considerable loss of galactolipid molecules forming monolayers was found at ozone concentrations (in aqueous medium) higher than 0.1 ppm with a stronger effect measured for MGDG. That goes along with the greater amounts of MDA found in the extracts of oxidized MGDG films compared with DGDG. Based on this, it was concluded that an additional galactose group present in DGDG molecules acts protectively under oxidative conditions. The surface tension of the solutions (of small volume) contacting the oxidized galactolipids films was significantly reduced, indicating the presence of soluble in polar media, surface active reaction products. The presence of α-tocopherol in mixtures with tested galactolipids at a molar ratio of lipid to tocopherol equal to 1.7:1 caused some inhibition of lipid oxidation, reducing the decrease of amount of lipid particles forming the monolayer. Here, also protective effect of α-tocopherol was greater for the MGDG compared to DGDG.

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“…The quantity of MDA was determined by measuring absorbance of MDA/TBA adduct (Sochor et al 2012). Samples were added to 0.25 % TBA in 10 % trichloroacetic acid.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Aspects of Reaction of Ozone with Galactolipid and Galactolipid–Tocopherol Layers

2014

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“…In fact, in comparison to the saturated fatty acids (with no double bonds) and monounsaturated fatty acids (with one double bond), PUFAs are more vulnerable to ROSmediated peroxidation because of the presence of greater number of double bonds in a fatty-acid chain, and the easy removal of a hydrogen atom (Wagner et al 1994;Porter et al 1995). Owing to their unstable nature, lipoperoxides decompose to form a wide range of compounds including the reactive carbonyl compounds, especially certain aldehydes [such as malondialdehyde (MDA) and 4-hydroxy-2-nonenal or hydroxynonenal (4-HNE)], which in turn fetch severe consequences to cells by binding free amino groups of amino acids of proteins (reviewed by Sochor et al 2012). With particular reference to mitochondria, lipid peroxidation principally refers to peroxidation of PUFA of membrane lipids such as linoleic acid, linolenic acid and arachidonic acid, where various cytotoxic aldehydes, alkenals, and hydroxyalkenals can be yielded (Taylor et al 2004).…”

Section: Lipid Peroxidation In Plantsmentioning

confidence: 99%

“…Enzymatic lipid peroxidation is catalyzed by the enzymes lipoxygenase (LOX, EC 1.13.11.12) and cyclooxygenase (EC 1.14.99.1), which are involved in the formation of eicosanoids, which represent a group of biologically active lipid compounds derived from unsaturated fatty acids containing 20 carbon atoms (reviewed by Sochor et al 2012). In particular, LOXs (linoleate/oxygen oxidoreductases) are ubiquitously occurring non-heme Fe-containing fatty acid dioxygenases, soluble in water, constituted by a single polypeptide associated with an atom of Fe(III), and catalyze the Gill and Tuteja 2010;Sharma et al 2012;Sabater and Martin 2013) addition of molecular oxygen to PUFAs via regio-and stereospecific oxygenation, and thereby produce hydroperoxy fatty acids and oxy-free radicals (Garder 1991;Sofo et al 2004a).…”

Section: Lipid Peroxidation In Plantsmentioning

confidence: 99%

“…In another instance, modified FOX2 and iodometric assays were applied to detect LHP in methanol extract of a range of plant tissues including the pericarp (avocado; Persea americana; European pear, Pyrus communis), periderm (potato; Solanum tuberosum), leaves (cabbage, Fig. 4 Schematic representation of the basic mechanism of reactive oxygen species (ROS)-mediated peroxidation (oxidative/ non-enzymatic modification) of lipids (based on Gutteridge 1995;Södergren 2000;Mäkinen 2002;Sochor et al 2012) Brassica oleracea convar. capitata var.…”

Section: Ferrous Oxidation In Xylenol-orange Assaymentioning

confidence: 99%

“…Owing to the short-lived nature of toxic intermediates and ROS, and to the inefficiency of methodologies for their direct estimation, the quantification of stable end-products of reactions of the ROS (and their reaction products) with cellular macromolecules has been advocated as an alternative approach (Orhan et al 2004). In this context, plant-tissue contents of malondialdehyde (MDA, one of the final products of peroxidation of unsaturated fatty acids in phospholipids) and carbonylated proteins [reactive carbonyls (RCs)] are considered as the biochemical markers of lipid peroxidation/cell-membrane damage (Taulavuori et al 2001;Sochor et al 2012) and protein oxidation (Møller and Kristensen 2004), respectively. Increased peroxidation (degradation) of lipids and the elevated protein oxidation are common in plants growing under environmental stresses (Romero Puertas et al 2002;Han et al 2009;Tanou et al 2009;Mishra et al 2011;reviewed by Sharma et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

Anjum

Sofo

Scopa

et al. 2014

Environ Sci Pollut Res

258

144

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Stress factors provoke enhanced production of reactive oxygen species (ROS) in plants. ROS that escape antioxidant-mediated scavenging/detoxification react with biomolecules such as cellular lipids and proteins and cause irreversible damage to the structure of these molecules, initiate their oxidation, and subsequently inactivate key cellular functions. The lipid- and protein-oxidation products are considered as the significant oxidative stress biomarkers in stressed plants. Also, there exists an abundance of information on the abiotic stress-mediated elevations in the generation of ROS, and the modulation of lipid and protein oxidation in abiotic stressed plants. However, the available literature reflects a wide information gap on the mechanisms underlying lipid- and protein-oxidation processes, major techniques for the determination of lipid- and protein-oxidation products, and on critical cross-talks among these aspects. Based on recent reports, this article (a) introduces ROS and highlights their relationship with abiotic stress-caused consequences in crop plants, (b) examines critically the various physiological/biochemical aspects of oxidative damage to lipids (membrane lipids) and proteins in stressed crop plants, (c) summarizes the principles of current technologies used to evaluate the extent of lipid and protein oxidation, (d) synthesizes major outcomes of studies on lipid and protein oxidation in plants under abiotic stress, and finally, (e) considers a brief cross-talk on the ROS-accrued lipid and protein oxidation, pointing to the aspects unexplored so far.

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