Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure

Saito, Makoto; Matsumoto, M.; Funabashi, Mitsuru

doi:10.1016/0168-1605(92)90018-x

Cited by 34 publications

(21 citation statements)

References 11 publications

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“…A value of Ͻ10/g was assigned when no hybridization signals were obtained. (2,3,6,8,13,14,21,23). With the HGMF-CH method, up to 1,600 separate colonies on a single membrane can be simultaneously screened for the presence of the cpe gene.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, due to the lack of a specific method to isolate cpepositive C. perfringens from clinical and environmental samples, the present knowledge of the epidemiology of the cpepositive strains is deficient. Several PCR methods for the detection of cpe-positive C. perfringens from fecal samples have been described (6,8,13,21,23). The enumeration of cpepositive C. perfringens is obtained by nested PCR combined with the most-probable number (MPN) technique (14).…”

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confidence: 99%

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Enumeration and Isolation of cpe -Positive Clostridium perfringens Spores from Feces

2004

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Enumeration and Isolation of cpe -Positive Clostridium perfringens Spores from Feces

2004

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“…A summary of diagnostic accuracy from individual studies of HipO Campylobacter PCR is shown in 86 other samples. The test performed well with the small number of samples on which it was tested; however, the kit is produced for research use only, and is not available for routine diagnostic use.…”

Section: Sroc Analysismentioning

confidence: 99%

A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food

Abubakar¹,

Irvine²,

Aldus³

et al. 2007

Health Technol Assess

103

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“…27 28 For example, Saito et al successfully used the supernatant of sporulation cultures of faeces as template DNA to detect cpe by PCR. 28 Further work is needed to overcome the problems associated with the extraction and amplification of C perfringens DNA from stool samples. Resolution of this problem could render PCR an important tool for understanding more about C perfringens diarrhoeal diseases.…”

Section: Diagnosismentioning

confidence: 99%

Evidence for antibiotic induced Clostridium perfringens diarrhoea

Modi¹,

Wilcox²

2001

Journal of Clinical Pathology

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Clostridium diYcile is a well documented cause of antibiotic associated diarrhoea in hospitalised patients, but may account for only approximately 20% of all cases. This leader reviews the current knowledge and understanding of the pathogenesis, epidemiology, and diagnosis of non-food borne Clostridium perfringens diarrhoea. Although enterotoxigenic C perfringens has been implicated in some C diYcile negative cases of antibiotic associated diarrhoea, C perfringens enterotoxin detection methods are not part of the routine laboratory investigation of such cases. Testing for C perfringens enterotoxin in faecal samples from patients with antibiotic associated diarrhoea and sporadic diarrhoea on a routine basis would have considerable resource implications. Therefore, criteria for initiating investigations and optimum laboratory tests need to be established. In addition, establishing the true burden of C perfringens antibiotic associated diarrhoea is important before optimum control and treatment measures can be defined. (J Clin Pathol 2001;54:748-751)

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Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure

Cited by 34 publications

References 11 publications

Enumeration and Isolation of cpe -Positive Clostridium perfringens Spores from Feces

Enumeration and Isolation of cpe -Positive Clostridium perfringens Spores from Feces

A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food

Evidence for antibiotic induced Clostridium perfringens diarrhoea

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