Enumeration and Isolation of
            <i>cpe</i>
            -Positive
            <i>Clostridium perfringens</i>
            Spores from Feces

Heikinheimo, Annamari; Lindström, Miia; Korkeala, Hannu

doi:10.1128/jcm.42.9.3992-3997.2004

Cited by 21 publications

(19 citation statements)

References 22 publications

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“…Several studies have indicated that cpe ‐positive strains occur in low numbers in co‐existence with large numbers of cpe ‐negative C. perfringens in the intestines of humans and animals (Miwa et al. 1997; Heikinheimo et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the broiler chicken isolates were obtained from conventionally cultivated samples by picking a couple of colonies from each sample, thus the isolates represented the dominant C. perfringens population in the broiler intestinal samples. In the future, by using a specific method for detection and isolation of cpe ‐positive C. perfringens strains (Heikinheimo et al. 2004), the cpe ‐positive C. perfringens strains could be detected and isolated from the dominating cpe ‐negative C. perfringens population, and the presence of the cpe gene and the type of the isolate could be further confirmed with the multiplex PCR assay.…”

Section: Discussionmentioning

confidence: 99%

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Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Heikinheimo

Korkeala

2005

Lett Appl Microbiol

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Aims: The aim of the study was to determine the presence of genes coding for alpha (cpa), beta (cpb), epsilon (etx), iota (iA) and enterotoxin (cpe) from Clostridium perfringens broiler chicken isolates, using multiplex PCR assay established in the study. Methods and Results: The multiplex PCR assay was shown to be specific when tested with 10 C. perfringens strains representing different toxin types, and 15 strains of other bacterial species. All 118 broiler chicken C. perfringens isolates were shown to carry the cpa gene but not cpb, etx, iap or cpe genes, signifying that all isolates represented type A and were cpe-negative. Conclusions: The assay established in the study enables the simultaneous detection of the major toxin genes and the cpe gene from C. perfringens isolates. Significance and Impact of the Study: The present study offers a new primer pair for detecting cpa, combined with a multiplex PCR assay. In addition, the study provides data of the presence of different toxin genes in C. perfringens isolates obtained from broiler chickens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Heikinheimo

Korkeala

2005

Lett Appl Microbiol

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“…Since both CPEnt gene-positive and -negative C. perfringens isolates may be present simultaneously in an individual and the ratio of CPEnt-positive to total C. perfingens strains is presumably low [15], non-enterotoxigenic isolates may have been picked for subcultures resulting in false-negative PCR results. In addition, C. perfringens strains could have lost their CPEnt gene during subculture procedures [31].…”

Section: Discussionmentioning

confidence: 98%

“…Non-toxigenic strains of C. perfringens can be usually found in the gut flora of healthy individuals in small numbers (up to 10 3 colony forming units [CFU]/g stool) [14]. In the stool of symptomatic patients, ≥ 10 6 spores of C. perfringens per g stool can be detected [14]; the ratio of CPEnt positive to total C. perfringens strains is estimated to be low [15].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Clostridium difficile Toxins A and B and Clostridium perfringens Enterotoxin A in Stool Samples of Patients with Antibiotic–Associated Diarrhea

et al. 2005

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“…Aunque era de esperar que el porcentaje de cepas toxigénicas fuera bajo, es posible que las cepas hayan correspondido, por azar, a cepas no toxigénicas, porque en una misma muestra puede coexistir un pequeño número de células cpe-positivas con un gran número de células cpe-negativas (Miwa et al 1997, Heikinheimo et al 2004, Heikinheimo et al 2006.…”

Section: Discussionunclassified

Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica

Gamboa-Coronado

Mau-Inchaustegui

Rodríguez-Cavallini

2010

RBT

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Molecular characterization and antimicrobial resistance of Clostridium perfringens isolates of different origins from Costa Rica. Clostridium perfringens, a Gram positive, spore-forming anaerobe, is widely distributed in nature. Based upon their production of four major toxins α, β, ε and ι, C. perfringens is classified into five toxinotypes (A-E). Some strains produce an enterotoxin (CPE), encoded by the cpe gene, which causes diarrhea in humans and some animals. C. perfringens strains that had been previously isolated and been kept at -80°C were analyzed for the presence of toxin genes and for antimicrobial resistance: 20 from soils, 20 from animal, 20 from human origin and 21 from food non related to outbreaks. According to PCR results, all strains were classified as C. perfringens type A, since only α toxin gene was detected, while cpe was detected in two strains (2.5%) isolated from food, as it has been described in other world regions. Antibiotic resistance to at least one antibiotic was detected in 44% of the strains, 41% was resistant to clindamycin, 25% to chloramphenicol, 22% to penicillin and 20% to metronidazole. Soils strains showed the highest resistance percentages to almost all antibiotics. Multiresistance (to three or more antibiotic groups) was detected in the strains from soil (40%), human origin (30%), food (14%) and animal origin (5%). The high resistance rates found may be explained by the widespread use of antimicrobials as growth promoters in plants and animals; also these resistant strains may act as reservoir of resistance genes that may be transferred between bacteria in different environments. Rev. Biol. Trop. 59 (4): 1479-1485. Epub 2011 December 01.

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Enumeration and Isolation of cpe -Positive Clostridium perfringens Spores from Feces

Cited by 21 publications

References 22 publications

Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Multiplex PCR assay for toxinotyping Clostridium perfringens isolates obtained from Finnish broiler chickens

Prevalence of Clostridium difficile Toxins A and B and Clostridium perfringens Enterotoxin A in Stool Samples of Patients with Antibiotic–Associated Diarrhea

Caracterización molecular y resistencia antimicrobiana de aislamientos de Clostridium perfringens de diferentes orígenes en Costa Rica

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