Detection of clinical interactions between methadone and anti-retroviral compounds using an enantioselective capillary electrophoresis for methadone analysis

Esteban, Javier; Pellín, María de la Cruz; Gimeno, Carmen; Barril, J.; Mora, Eva; Giménez, Jesús; Vilanova, Eugenio

doi:10.1016/j.toxlet.2004.04.007

Cited by 20 publications

(8 citation statements)

References 19 publications

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“…Compared to the previous decade, during which mostly uncharched CDs were employed [26], we noted a strong increase of the use of charged CDs. S-␤-CD, with 7-11 mol sulfate/mol ␤-CD, was most often used [58,60,63,69,71,79,80,83,95,97,99,101,108,110,118,123,131,134,135,139,[154][155][156][157][158][159][160][161][162][163][164][165][166][167][168][169] whereas the better defined highly sulfated CD derivatives (HS-␤-CD and HS-␥-CD [170]) were only used in a few assays [50,51,72,111,144,145]. These and other selectors, such as phosphated-␥-CD (PH-␥-CD) [94,106,126], carboxymethyl-␤-CD (CM-␤-CD) or carboxymethyl-␥-CD (CM-␥-CD) …”

Section: Principlesmentioning

confidence: 99%

“…Using a pH 2.7 buffer with a dual CD system comprising 2 mM CM-␤-CD and 4 mM dimethyl-␤-CD (DM-␤-CD), analysis of liquid/liquid extracts of rat urine revealed that the N-oxidation of the two deprenyl enantiomers occurs in a stereoselective manner. Other studies included the assessment of the impact of antiviral drugs on the metabolism of methadone for which monitoring of the R-enantiomer, the enantiomer with the much higher therapeutic effect compared to S-methadone, is of clinical interest [111], the ketoreduction of oxycodone and noroxycodone to oxycodol and noroxycodol, respectively, processes in which diastereoisomers are formed [119], and the hydroxylation of itraconazole [108]. In all these studies, stereoselectivity was assessed via a stereoisomeric ratio, e.g.…”

Section: Assessment Of Stereoselective Pharmacokinetics and Drug Metamentioning

confidence: 99%

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Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

Caslavska

Thormann

2011

Journal of Chromatography A

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Section: Principlesmentioning

confidence: 99%

Section: Assessment Of Stereoselective Pharmacokinetics and Drug Metamentioning

confidence: 99%

Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

Caslavska

Thormann

2011

Journal of Chromatography A

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“…Published enantioselective assays for the monitoring of methadone or methadone and EDDP in biological samples are based on GC‐MS , HPLC with UV detection , LC‐MS , CE with UV detection and CE‐MS . Enantiomeric CE is based upon interaction between analytes and one or multiple chiral selectors which are added to the running buffer and was successfully applied to bioanalytical drug monitoring .…”

Section: Introductionmentioning

confidence: 99%

“…While it is straightforward to separate methadone enantiomers by CE in presence of neutral cyclodextrins, resolution of the EDDP stereoisomers is difficult with these chiral selectors . With sulfated cyclodextrins, however, both methadone and EDDP enantiomers separate easily .…”

Section: Introductionmentioning

confidence: 99%

Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane

et al. 2019

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An enantioselective assay for the determination of methadone and its main metabolite 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine in equine plasma based on capillary electrophoresis with highly sulfated γ‐cyclodextrin as chiral selector and electrokinetic analyte injection is described. The assay is based on liquid/liquid extraction of the analytes at alkaline pH from 0.1 mL plasma followed by electrokinetic sample injection of the analytes from the extract across a buffer plug without chiral selector. Separation occurs cationically at normal polarity in a pH 3 phosphate buffer containing 0.16% (w/v) of highly sulfated γ‐cyclodextrin. The developed assay is precise (intra‐ and interday RSD < 4% and < 7%, respectively), is capable to determine enantiomer levels of methadone and 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine in plasma down to 2.5 ng/mL, and was successfully applied to monitor enantiomer drug and metabolite levels in plasma of a pony that was anesthetized with racemic ketamine and isoflurane and received a bolus of racemic methadone and a bolus followed by constant rate infusion of racemic methadone. The data suggest that the assay is well suited for pharmacokinetic purposes.

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“…Chiral separations may also be of use for monitoring therapeutic drug concentrations of methadone (where the R-enantiomer dominates with respect to therapeutic effect) [86], and for monitoring new imidazole compounds that are under investigation for aromatase-inhibitor activities [87]. Another interesting study showed that it may be possible to decouple enantioselectivity from the effect of dimerization for given separation patterns.…”

mentioning

confidence: 99%

Recent applications of affinity interactions in capillary electrophoresis

2006

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Systems biology depends on a comprehensive assignment and characterization of the interactions of proteins and polypeptides (functional proteomics) and of other classes of biomolecules in a given organism. High-capacity screening methods are in place for ligand capture and interaction screening, but a detailed dynamic characterization of molecular interactions under physiological conditions in efficiently separated mixtures with minimal sample consumption is presently provided only by electrophoretic interaction analysis in capillaries, affinity CE (ACE). This has been realized in different fields of biology and analytical chemistry, and the resulting advances and uses of ACE during the last 2.5 years are covered in this review. Dealing with anything from small divalent metal ions to large supramolecular assemblies, the applications of ACE span from low-affinity binding of broad specificity being exploited in optimizing selectivity, e.g., in enantiomer analysis to miniaturized affinity technologies, e.g., for fast processing immunoassay. Also, approaches that provide detailed quantitative characterization of analyte-ligand interaction for drug, immunoassay, and aptamer development are increasingly important, but various approaches to ACE are more and more generally applied in biological research. In addition, the present overview emphasizes that distinct challenges regarding sensitivity, parallel processing, information-rich detection, interfacing with MS, analyte recovery, and preparative capabilities remain. This will be addressed by future technological improvements that will ensure continuing new applications of ACE in the years to come.

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Detection of clinical interactions between methadone and anti-retroviral compounds using an enantioselective capillary electrophoresis for methadone analysis

Cited by 20 publications

References 19 publications

Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane

Recent applications of affinity interactions in capillary electrophoresis

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