Detection of Circulatory MicroRNAs in Prostate Cancer

Srivastava, Anshu; Goldberger, Helle; Afzal, Zainab; Suy, Simeng; Collins, Sean P.; Kumar, Deepak

doi:10.1007/978-1-4939-1804-1_27

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…This confirmed the previous report of an increased mi-R141 by Mattie et al [211]. Srivastava et al [212] have listed twelve miRNAs with relevance to prostate cancer. However, they advise caution concerning placing too much emphasis on their use until further work has been performed to ensure their viability.…”

Section: Prostate Cancer (Pc)supporting

confidence: 90%

Biology of Circulating Nucleic Acids and Possible Roles in Diagnosis and Treatment in Diabetes and Cancer

Gahan

2012

IDDT

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The presence of DNA and RNA circulating in human plasma and serum is described. The possible sources of the DNA/RNA in blood, their ability to enter other cells and to express in the recipient cells are discussed and the relationship with metastases considered. The possible role(s) of the DNA/RNA in clinical diagnosis, in monitoring treatment and in prognosis are considered for diabetes and oncology.

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Section: Prostate Cancer (Pc)supporting

confidence: 90%

Biology of Circulating Nucleic Acids and Possible Roles in Diagnosis and Treatment in Diabetes and Cancer

Gahan

2012

IDDT

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“…RNA extraction from microdissected cells and serum samples was performed essentially as described earlier [ 15 , 16 , 18 ]. RNA was quantified using NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-212 negatively regulates starvation induced autophagy in prostate cancer cells by inhibiting SIRT1 and is a modulator of angiogenesis and cellular senescence

et al. 2015

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Among a number of non-coding RNAs, role of microRNAs (miRNAs) in cancer cell proliferation, cancer initiation, development and metastasis have been extensively studied and miRNA based therapeutic approaches are being pursued. Prostate cancer (PCa) is a major health concern and several deregulated miRNAs have been described in PCa. miR-212 is differentially modulated in multiple cancers however its function remains elusive. In this study, we found that miR-212 is downregulated in PCa tissues when compared with benign adjacent regions (n = 40). Also, we observed reduced levels of circulatory miR-212 in serum from PCa patients (n = 40) when compared with healthy controls (n = 32). Elucidating the functional role of miR-212, we demonstrate that miR-212 negatively modulates starvation induced autophagy in PCa cells by targeting sirtuin 1 (SIRT1). Overexpression of miR-212 also leads to inhibition of angiogenesis and cellular senescence. In conclusion, our study indicates a functional role of miR-212 in PCa and suggests the development of miR-212 based therapies.

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“…According to these authors, results did not improve when the exogenous controls cel-miR-54 and cel-miR-238 to celmiR-39 were added. Exogenous miRNAs controls, such as ath-miR-159a, cel-miR-54, cel-miR-238 and particularly celmiR-39, appear as the optimal way to normalize miRNAs expression in absence of an ideal endogenous control [44,45], although biological variability should be taken into account to improve miRNAs measurement.…”

Section: Preanalytical and Analytical Variables In Mirna Measurementmentioning

confidence: 99%

miRNAs as novel biomarkers in the management of prostate cancer

Filella

Foj

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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microRNAs (miRNAs) are small non-coding RNAs that control gene expression posttranscriptionally and are part of the giant non codifying genoma. Cumulating data suggest that miRNAs are promising potential biomarkers for many diseases, including cancer. Prostate cancer (PCa) detection is currently based in the serum prostate-specific antigen biomarker and digital rectal examination. However, these methods are limited by a low predictive value and the adverse consequences associated with overdiagnosis and overtreatment. New biomarkers that could be used for PCa detection and prognosis are still needed. Recent studies have demonstrated that aberrant expressions of microRNAs are associated with the underlying mechanisms of PCa. This review attempts to extensively summarize the current knowledge of miRNA expression patterns, as well as their targets and involvement in PCa pathogenesis. We focused our review in the value of circulating and urine miRNAs as biomarkers in PCa patients, highlighting the existing discrepancies between different studies, probably associated with the important methodological issues related to their quantitation and normalization. The majority of studies have been performed in serum or plasma, but urine obtained after prostate massage appears as a new way to explore the usefulness of miRNAs. Large screening studies to select a miRNA profile have been completed, but bioinformatics tools appear as a new approach to select miRNAs that are relevant in PCa development.Promising preliminary results were published concerning miR-141, miR-375 and miR-21, but larger and prospective studies using standardized methodology are necessary to define the value of miRNAs in the detection and prognosis of PCa.

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Detection of Circulatory MicroRNAs in Prostate Cancer

Cited by 8 publications

References 30 publications

Biology of Circulating Nucleic Acids and Possible Roles in Diagnosis and Treatment in Diabetes and Cancer

Biology of Circulating Nucleic Acids and Possible Roles in Diagnosis and Treatment in Diabetes and Cancer

MicroRNA-212 negatively regulates starvation induced autophagy in prostate cancer cells by inhibiting SIRT1 and is a modulator of angiogenesis and cellular senescence

miRNAs as novel biomarkers in the management of prostate cancer

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