Detection of cefquinome in milk by liquid chromatography and screening methods

Suhren, G.; Knappstein, Karin

doi:10.1016/s0003-2670(02)01473-3

Cited by 23 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, a number of methods for the determination of cefquinome were reported for milk and animal tissues. These methods include high-performance liquid chromatography with UV detector (HPLC-UV) (17,18,21), HPLC with diode array detector (5), liquid chromatography combined with tandem mass spectrometry (1), and screening methods (7,8,13,14,18). To the authors' knowledge, only two published papers involving analytical methods for the determination of cefquinome in plasma, one employing liquid chromatography combined with electrospray tandem mass spectrometry (LC-ESI-MS-MS) (11) and one using HPLC-UV (9), reported complete validation procedures.…”

mentioning

confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Üney

Altan

Elmas

2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Cefquinome has a broad spectrum of antibacterial activity and was developed especially for use in animals. A simple and sensitive high-performance liquid chromatography (HPLC) method with UV-visible detection for quantification of cefquinome concentrations in sheep plasma was developed and validated. Separation of cefquinome from plasma components was achieved on a Phenomenex Gemini C 18 column (250 mm by 4.6 mm; internal diameter [i.d.], 5 m). The mobile phase consisted of acetonitrile and 0.1% trifluoroacetic acid in water and was delivered at a rate of 0.9 ml/min. A simple and rapid sample preparation involved the addition of methanol to 200 l of plasma to precipitate plasma proteins followed by direct injection of 50 l of supernatant into the high-performance liquid chromatography system. The linearity range of the proposed method was 0.02 to 12 g/ml. The intraday and interday coefficients of variation obtained from cefquinome were less than 5%, and biases ranged from ؊3.76% to 1.24%. Mean recovery based on low-, medium-, and high-quality control standards ranged between 92.0 and 93.9%. Plasma samples were found to be stable in various storage conditions (freeze-thaw, postpreparative, short-term, and long-term stability). The method described was found to be readily available, practicable, cheap, rapid, sensitive, precise, and accurate. It was successfully applied to the study of the pharmacokinetics of cefquinome in sheep. This method can be very useful and an alternate to performing pharmacokinetic studies in the determination of cefquinome for clinical use.

show abstract

mentioning

confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Üney

Altan

Elmas

2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Shantier's studies showed that cefquinome sulfate solutions are degraded via hydrolysis, which appears to depend on temperature and hydroxide ion concentration [13]. Chromatographic methods were used for the analysis of cefquinome sulfate in animal biological fluids and tissues [14][15][16]. The HPLC and TLC methods developed in this study were successfully used to separate the alkaline degradation products from the parent compound [17].…”

Section: Introductionmentioning

confidence: 99%

Critical parameters for the stability of cefquinome sulfate in aqueous solutions and solid phase

Dołhań

Urbaniak

Manuszewska

et al. 2017

Reac Kinet Mech Cat

View full text Add to dashboard Cite

Cefquinome sulfate is a veterinary, parenteral, fourth-generation cephalosporin with a methoxyimino-aminothiazolyl moiety into the acyl side chain and the quaternary quinoline group at position 3 of the cefem ring. Cefquinome sulfate is known to undergo degradation, which could be additionally increased by the presence of some critical factors such as temperature, relative air humidity, buffer components as well as hydrogen and hydroxide ions. The aim of this study was to evaluate the factors underlying the stability of cefquinome sulfate (CFQ) in aqueous solution and in the solid state taking into account general and specific acid-base hydrolysis in the pH range 0.45-10.48 at 343 K; the effect of temperature on the stability at 0% RH and an increased relative air humidity (RH = 76.4%); the influence of relative air humidity at 343 K (66.5-90%). To determine the observed rate constants, an isocratic HPLC-UV method was used. The three pK a values of cefquinome sulfate, kinetic (k obs. , k pH ), and activation parameters (E a , DH = , and DS = ) were determined.

show abstract

“…Besides its use in pigs, cefquinome is also indicated for treatment of clinical mastitis in lactating cows caused by Escherichia coli and Streptococcus and Staphylococcus species. 1,2 Apart from viruses, respiratory pathogens that are commonly found in pigs include Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida, Bordetella bronchiseptica and Streptococcus suis. The susceptibility of Ł Correspondence to: A. Maes, Department of Pharmacology, Toxicology, Biochemistry and Organ Physiology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.…”

Section: Introductionmentioning

confidence: 99%

“…3 Three procedures for the determination of cefquinome and other cephalosporins in milk using high-performance liquid chromatography (HPLC) with UV detection have been described. 1,2,4 Moreover, a multiresidue method for analysis of 15 penicillins and cephalosporins in bovine tissues (muscle and kidney) and milk using LC-MS/MS has also been published. 5 The extraction procedure described in almost all of these methods is based on a liquid extraction followed by a solid phase extraction.…”

Section: Introductionmentioning

confidence: 99%

Determination of cefquinome in pig plasma and bronchoalveolar lavage fluid by high‐performance liquid chromatography combined with electrospray ionization mass spectrometry

Maes

Meyns

Sustronck³

et al. 2007

J. Mass Spectrom.

View full text Add to dashboard Cite

The aim of this study was to develop a rapid and sensitive method for the quantification of cefquinome in animal plasma and bronchoalveolar lavage (BAL) fluid using high-performance liquid chromatography combined with electrospray tandem mass spectrometry (LC-ESI-MS/MS). Cefadroxil is used as internal standard. For plasma, the sample preparation includes a simple deproteinization step with a Microcon filter. This allows detecting the unbound cefquinome concentration, which is correlated with the concentration in other body fluids, such as BAL fluid. To be able to detect the total plasma concentration, deproteinization with acetonitrile, followed by a back-extraction of actonitrile with dichloromethane was performed. The BAL fluid is centrifuged to precipitate floating particles. Chromatographic separation is achieved on a PLRP-S column using 0.005% formic acid and methanol as mobile phase. For plasma, good linearity was observed in the range of 5-2500 ng ml(-1) for both the unbound and total concentration. The response in BAL fluid was linear in the range of 4-1000 ng ml(-1). The limit of quantification (LOQ) was set at 5.00 ng ml(-1) for plasma and at 4.00 ng ml(-1) for BAL fluid. The limit of detection (LOD) was 3.12 ng ml(-1) and 0.41 ng ml(-1) for the unbound and total concentration in plasma, respectively, and was 1.43 ng ml(-1) for BAL fluid. The method was shown to be of use in a pharmacokinetic study in pigs, where the correlation between cefquinome concentrations in plasma and BAL fluid of pigs was studied.

show abstract

Detection of cefquinome in milk by liquid chromatography and screening methods

Cited by 23 publications

References 12 publications

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Critical parameters for the stability of cefquinome sulfate in aqueous solutions and solid phase

Determination of cefquinome in pig plasma and bronchoalveolar lavage fluid by high‐performance liquid chromatography combined with electrospray ionization mass spectrometry

Contact Info

Product

Resources

About