Detection of bovine viral diarrhoea virus nucleic acid, but not infectious virus, in bovine serum used for human vaccine manufacture

Laassri, Majid; Mee, Edward T.; Connaughton, Sarah M.; Manukyan, Hasmik; Gruber, Marion F.; Hernandez, Carmen A. Rodriguez; Minor, Philip D.; Schepelmann, Silke; Chumakov, Konstantin; Wood, David

doi:10.1016/j.biologicals.2018.06.002

Cited by 7 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some national and foreign companies offer non-irradiated FBS, which has potential detrimental consequences, such as contamination of cell cultures used in basic research, virological diagnosis, upstream processing of biopharmaceuticals, embryo technology, and other animal reproduction protocols. A clear evidence of these risks is the frequent findings of viral agents' traces in cell lines, pharmaceutics products and vaccines (13).…”

Section: Discussionmentioning

confidence: 99%

“…Although the commercialization of gamma-irradiated FBS is recommended, some companies market it without the irradiation process and this could lead to silent contamination of products in the research and industrial sector. Further, gamma-irradiation may not be 100% effective under all conditions (13). Besides Brazil, the epidemiology of Hobi-like virus in South America is unknown (14).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Pécora

Aguirreburualde

Ridpath³

et al. 2019

Front. Vet. Sci.

View full text Add to dashboard Cite

Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. Therefore, 124 local samples of non-irradiated FBS originating from Argentina were surveyed for the presence of pestiviruses using RT-PCR. Amplicons from pestivirus positive samples were genotyped. Four samples were positive for HoBi virus-specific RT-PCR, while the BVDV-positive samples (n = 45) were classified as BVDV-1b (82.2%), BVDV-1a (13.3%), and BVDV-2 (4.5%). Virus isolation and serological profile assessment were performed for the four HoBi-positive FBS lots. These results confirm the circulation of HoBi-like virus in some regions of the Argentinean territory, highlighting the need to review the diagnostic techniques currently used in the clinical cases suspected of BVDV and in contamination control protocols for adventitious agents in cells and biotechnological products.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Pécora

Aguirreburualde

Ridpath³

et al. 2019

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…The previous molecular methods were designed for specific detection of Sabin strains in clinical and environmental samples but cannot distinguish Sabin strains from their genetically modified nOPV derivatives [ 20 , 22 , 23 , 24 , 25 ]. Here we describe the assay for specific detection and quantitation of Sabin 2 virus even in the presence of relevant amounts of nOPV2 virus.…”

Section: Discussionmentioning

confidence: 99%

“…Several molecular-based procedures have been developed for detection of OPV strains, including ELISA [ 20 ], reverse transcription-polymerase chain reaction (RT-PCR) followed by hybridization with specific oligonucleotides [ 21 , 22 ], and quantitative RT-PCR [ 23 ] that uses degenerate primers with mixed-base and inosine residues. Recently we have developed quantitative one-step RT-PCR (qosRT-PCR) and quantitative multiplex one-step RT-PCR (qmosRT-PCR) assays for detection and quantitation of the three Sabin strains of OPV [ 24 , 25 ]. None of these methods were designed to specifically identify Sabin strains in the presence of nOPV.…”

Section: Introductionmentioning

confidence: 99%

Development of a Quantitative One-Step RT-PCR Method for the Detection of Sabin 2 Virus Contamination in a Novel Oral Poliovirus Vaccine Type 2

et al. 2021

Self Cite

View full text Add to dashboard Cite

To control circulating vaccine-derived type 2 poliovirus outbreaks, a more genetically stable novel Oral Poliovirus Vaccine type 2 (nOPV2) was developed by targeted modifications of Sabin 2 genome. Since the use of OPV2 made of Sabin 2 strain has been stopped, it is important to exclude the possibility that batches of nOPV2 are contaminated with Sabin 2 virus. Here, we report the development of a simple quantitative one-step reverse-transcription polymerase chain reaction assay for the detection and quantitation of Sabin 2 virus in the presence of overwhelming amounts of nOPV2 strain. The method is specific and linear within 8 log10 range even in the presence of relevant amounts of nOPV2 virus. It is sensitive, with a lower limit of detection of 0.2 CCID50/mL (an equivalent of 198 genome copies per mL), and generates reproducible results. This assay can be used for quality control and lot release of the nOPV2.

show abstract

“…However, a high concentration of FBS is not recommended in vaccine seed virus storage due to its high cost; moreover, FBS might inhibit infectivity of the virus (Iki et al ). Additionally, nucleic acids of adventitious virus such as bovine viral diarrhoea virus stored in FBS might alter the result of the contamination test conducted on vaccine seed virus despite performing virus inactivation treatment of FBS (Laassri et al ). Sucrose is well known as a cryo‐protectant for enveloped viruses, such as varicella‐zoster virus, respiratory syncytial virus and herpes simplex virus, as it might stabilize infectivity by preventing conformational changes in the viral envelope structure (Munk and Ackermann ; Law and Hull ; Grose et al ).…”

Section: Introductionmentioning

confidence: 99%

Three‐percent sucrose acts as a thermostabilizer for cell‐adapted foot‐and‐mouth disease virus without any negative effect on viral growth

et al. 2020

View full text Add to dashboard Cite

Aims: As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. Methods and Results: We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell-adapted FMDV O/SKR/JC/2014, containing H56R mutation in VP3, as vaccine seed virus at À80, 4, 25 or 37°C for 2, 4 or 7 days. The stabilizing effect of 3% sucrose on O/SKR/JC/2014 was observed at 25, 37°C, and after repeated freeze-thaw cycles. Additionally, we tested the effect of 3% sucrose on the growth of FMDV or cells and did not observe any decrease in either viral growth or cell viability. Conclusions: Our study showed the protective effect of 3% sucrose on FMDV infectivity at various temperatures; this virus stock in 3% sucrose could be used for infecting cells without the removal of sucrose. Significance and Impact of the Study: We suggest that 3% sucrose-containing medium could be beneficial for the stable storage and transport of cell-adapted FMDV.

show abstract

Detection of bovine viral diarrhoea virus nucleic acid, but not infectious virus, in bovine serum used for human vaccine manufacture

Cited by 7 publications

References 29 publications

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Development of a Quantitative One-Step RT-PCR Method for the Detection of Sabin 2 Virus Contamination in a Novel Oral Poliovirus Vaccine Type 2

Three‐percent sucrose acts as a thermostabilizer for cell‐adapted foot‐and‐mouth disease virus without any negative effect on viral growth

Contact Info

Product

Resources

About