Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Pécora, Andrea; Aguirreburualde, María Sol Pérez; Ridpath, Julia F.; Santos, María José Dus

doi:10.3389/fvets.2019.00359

Cited by 20 publications

(17 citation statements)

References 37 publications

(41 reference statements)

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“…Thus, the BVDV subgenotypes detected in these biological samples are similar, and this correlates with the diversity detected in the animal population of Mexico [ 3 ]. These results were consistent with a study conducted in Argentina, where an analysis of FBS samples resulted in the identification of BVDV-1a, 1b, 2a, and HoBi-like viruses in that country [ 31 ]. This suggests that analysis of lots of FBS produced in Mexico on a regular basis may be a valuable tool for detecting the circulation of new pestiviruses in this country.…”

supporting

confidence: 92%

Detection and genotyping of bovine viral diarrhea virus found contaminating commercial veterinary vaccines, cell lines, and fetal bovine serum lots originating in Mexico

Gómez‐Romero

Velazquez‐Salinas

Ridpath³

et al. 2021

Arch Virol

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In this communication, we report the presence of RNA of bovine viral diarrhea virus (BVDV) as a contaminant of different biological products used in Mexico for veterinary vaccine production. For this purpose, six batches of monovalent vaccines, eight cell line batches used for vaccine production, and 10 fetal bovine serum lots (FBS) commercially available in Mexico from different suppliers were tested by reverse transcription polymerase chain reaction (RT-PCR). Viral RNA was detected in 62.5% of the samples analyzed. Phylogenetic analysis revealed the presence of the subgenotypes BVDV-1a, 1b, and BVDV-2a in the tested samples. Collectively, these findings indicate that contamination by BVDV RNA occurs in commercial vaccines and reagents used in research and production of biological products. The ramifications of this contamination are discussed.

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supporting

confidence: 92%

Detection and genotyping of bovine viral diarrhea virus found contaminating commercial veterinary vaccines, cell lines, and fetal bovine serum lots originating in Mexico

Gómez‐Romero

Velazquez‐Salinas

Ridpath³

et al. 2021

Arch Virol

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“…Northern Italy was estimated to be a source area to other parts of the country of subgenotypes that are now widespread at the national level (19,24). In addition, biological products contaminated by fetal bovine serum have to be considered as possible source for introduction of bovine pestivirus species and subgenotypes into new areas (6,31,56). A data integration of the cattle movement dataset with the pestivirus status is advisable to optimize the outcome of molecular characterization of pestiviruses, performing an accurate contact tracing among farms and investigating transmission pathways among different areas.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Bovine Pestiviruses Circulating in Italy

Luzzago

Decaro

2021

Front. Vet. Sci.

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Pestiviruses are widespread and economically important pathogens of cattle and other animals. Pestivirus A (formerly known as Bovine viral diarrhea virus 1, BVDV-1), Pestivirus B (Bovine viral diarrhea virus 2, BVDV-2), and Pestivirus H (HoBi-like pestivirus, HoBiPeV) species are infecting primarily cattle. Like other RNA viruses, pestiviruses are characterized by a high degree of genetic variability. This high rate of variability is revealed by the existence of a number of viral subgenotypes within each species. In cattle, the highest number of pestivirus subgenotypes has been documented in European countries, particularly in Italy. The aim of this review is to report an up-to-date overview about the genetic diversity of pestiviruses in Italian cattle herds. All three bovine pestiviruses species have been identified in cattle population with variable frequency and geographical distribution. The genetic diversity of Italian pestiviral strains may have diagnostic and immunological implications, affecting the performance of diagnostic tools and the full cross-protection elicited by commercially available vaccines. Implementation and strengthening of coordinated approaches for bovine pestivirus control in Italy are recommended. Therefore, it would be extremely important to increase control and restriction measures to the trade of cattle and biological products of bovine origin, including those containing fetal bovine serum.

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“…All the strains identified were typed as BVDV-1 according to the primers designed by Gilbert et al [16]. Previous studies described that around 90% of the strains in Argentina were BVDV-1 [31][32][33][34]. Although type 2 was not identified in the tested samples, the primers used here were able to detect this BVDV type in Argentinean field isolates [34].…”

Section: Discussionmentioning

confidence: 85%

Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study

et al. 2020

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Bovine viral diarrhea (BVD) is a major worldwide disease with negative economic impact on cattle production. Successful control programs of BVD require the identification and culling of persistently infected (PI) animals with bovine viral diarrhea virus (BVDV). A variety of diagnostic tests are available to detect BVDV, but no comparison has been performed among those tests in Argentina. Sera collected from 2864 cattle, belonging to 55 herds from three Argentinean provinces, were analyzed by nested RT-PCR (RT-nPCR) to detect BVDV for diagnostic purposes. Additionally, this study evaluated the agreement of the RT-nPCR along with virus isolation, antigen-capture ELISA, and real-time RT-PCR for BVDV detection in archived bovine serum samples (n = 90). The RT-nPCR was useful for BVDV detection in pooled and individual serum samples. BVDV was detected in 1% (29/2864) of the cattle and in 20% (11/55) of the herds. The proportion of BVDV-positive sera was not statistically different among the tests. In addition, comparisons showed high agreement levels, with the highest values between both RT-PCR protocols. The frequency of BVDV infection at individual and herd level was lower than the reported values worldwide. Since follow-up testing was not performed, the frequency of PI cattle was unknown. Also, this study demonstrated that the four diagnostic tests can be used reliably for BVDV identification in individual serum samples. Further epidemiologically designed studies that address prevalence, risk factors, and economic impact of BVDV in Argentina will be necessary to implement effective control programs.

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Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Cited by 20 publications

References 37 publications

Detection and genotyping of bovine viral diarrhea virus found contaminating commercial veterinary vaccines, cell lines, and fetal bovine serum lots originating in Mexico

Detection and genotyping of bovine viral diarrhea virus found contaminating commercial veterinary vaccines, cell lines, and fetal bovine serum lots originating in Mexico

Epidemiology of Bovine Pestiviruses Circulating in Italy

Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study

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