Three‐percent sucrose acts as a thermostabilizer for cell‐adapted foot‐and‐mouth disease virus without any negative effect on viral growth

Hwang, Ji Hyeon; Moon, Youn-Gi; Lee, G.; Kim, M.‐Y.; Lee, K.‐N.; Park, J.‐H.; Lee, M.; Kim, B.; Kim, Su-Mi

doi:10.1111/jam.14565

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Stabilizing additives such as disaccharides [17][18][19], amino acids [20], detergent [21], metal ions [19,22], etc. have been reported for various viruses, including FMDV, and research on stabilizing FMDVs by amino acid substitution of viral capsid proteins is being conducted worldwide [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation

Jin,

Lee,

Kim

et al. 2024

Vaccines

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The thermal stability of the in-house-developed foot-and-mouth disease (FMD) type O and A viruses was evaluated, and the O Jincheon virus was found to exhibit the lowest thermal stability. To overcome this instability, we proposed a novel stabilizer, calcium chloride. The thermal stability of FMDVs increased up to a CaCl2 concentration of 10 mM, and it had a decreasing trend at >30 mM. The O Jincheon virus showed a significant decrease in the amount of antigen over time at 4 °C. In contrast, the samples treated with CaCl2 showed stable preservation of the virus without significant antigen loss. After the CaCl2-formulated vaccine was administered twice to pigs, the virus neutralization titer reached approximately 1:1000, suggesting that the vaccine could protect pigs against the FMDV challenge. In summary, the O Jincheon virus is difficult to utilize as a vaccine given its low stability during storage after antigen production. However, following its treatment with CaCl2, it can be easily utilized as a vaccine. This study evaluated CaCl2 as a novel stabilizer in FMD vaccines and may contribute to the development of stable vaccine formulations, especially for inherently unstable FMDV strains.

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Section: Introductionmentioning

confidence: 99%

Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation

Jin,

Lee,

Kim

et al. 2024

Vaccines

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show abstract

“…Traditional thermostabilizers, including metal salts and organotin compounds, have been widely used to address these issues (Aisawa, 2019;Hwang, 2020;Kuzma-Kichta, 2020;Muhammed, 2019;Shabaev, 2019). However, these conventional stabilizers often come with drawbacks such as high cost, environmental concerns, and regulatory restrictions.…”

Section: Introductionmentioning

confidence: 99%

Thermostabilizer for Polyvinyl Chloride-development of Synergistic Compounds

Lutfullaev,

Tavashov,

Dostmuradova

2024

JME

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Thermostabilizer-synergistic mixtures with double salts of T-product for PVX were developed and their synergism efficiency phenomena were studied using DTA and TGA methods. The mixtures of thermostabilizers synergics of mixed salts of the product – T for PVCh were worked out by the methods of DTA and TGA and the effect of synergism was studied. This study aims to develop thermostabilizer-synergistic mixtures with double salts of T-product for polyvinyl chloride (PVC) and to investigate their synergistic efficiency using Differential Thermal Analysis (DTA) and Thermogravimetric Analysis (TGA) methods. The research involved synthesizing stabilizers, preparing PVC mixtures, conducting heat resistance tests, and analyzing the data obtained. The efficiency of the synergistic mixtures was evaluated through DTA and TGA to assess their impact on the thermal stability and degradation behavior of PVC. The study found that the synergistic mixtures significantly enhanced the thermal stability of PVC. The results indicated that the mixtures, particularly those involving lead salts of T-product, demonstrated a pronounced synergistic effect, delaying the onset of thermal degradation and improving overall stability. The effectiveness of the stabilizers was 2-3 times higher when used in mixtures compared to individual use. The optimal ratio for maximum stabilization efficiency was found to be 1:1, with specific mixtures showing an induction period of up to 120 minutes. The developed thermostabilizer-synergistic mixtures with double salts of T-product effectively enhance the thermal stability of PVC. The synergistic interactions between the components contribute to improved performance, making these mixtures promising candidates for enhancing the durability and lifespan of PVC materials. This advancement addresses critical issues related to environmental sustainability, performance enhancement, cost reduction, and market expansion, ensuring that PVC remains a viable and valuable material for a wide range of applications.

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Three‐percent sucrose acts as a thermostabilizer for cell‐adapted foot‐and‐mouth disease virus without any negative effect on viral growth

Cited by 2 publications

References 40 publications

Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation

Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation

Thermostabilizer for Polyvinyl Chloride-development of Synergistic Compounds

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