Detection of Biofilm Formation in some Pathogenic Bacteria Using Tube and Congo Red Agar Methods

Abstract

The research included studying the ability of some bacteria that were isolated from urinary catheter tips and urine samples in a previous study, to form biofilms by Congo red and tube method. The results showed that the highest biofilm formation using tube method was occurred by Citrobacter freundii, Escherichia coli and klebsiella spp.(100%), while Pseudomonas fluorescens did not form a biofilm. of gram positive bacteria, Staphylococcus aureus showed the highest biofilm formation (87.5%), while Micrococcus spp. showed the lowest level (33.3%). Using the congo red method in the gram positive bacteria, S.aureus showed biofilm formation of 31.25%, while S.epidermidis and Micrococcus spp. did not form biofilms. Using the same method for gram negative bacteria, E.coli showed a highest ability of (50%), while Ps.fluorescens and C.freundii did not form biofilms.

Allelopathic effect of some plants on morphological attributes of invasive alien weed: Malachra capitata (L.)L.

“…[hereafter it will be referred as R. communis] is a plant belonging to Euphorbiaceae, commonly found in the tropical and temperate climates of the world [9][10], which is well known for many of its medicinal and industrial uses [11][12]. Vitex negundo L. [hereafter it will be referred as V. negundo] an aromatic shrub belonging to verbenaceae widely known for its use as green manure, medicine in ayurvedic, unani systems of medicine and as a mosquito repellent.…”