Detection of Bacteroides fragilis Infection by Precipitin Antibody

Jp, Rissing; Jg, Crowder; Jw, Smith; White, A. Clinton

doi:10.1093/infdis/130.1.70

Cited by 26 publications

(9 citation statements)

References 8 publications

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“…fragilis serum reacting with non-B. fragilis antigen preparations within the B. fragilis group (2,3,5,9,17,26,27,30,31) or Bacteroides species not of the B. fragilis group (2,7,12). This current study suggests that F. nucleatum and C. ochraceus share antigenic determinants with B. fragilis.…”

Section: Discussionsupporting

confidence: 49%

Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Shaefer¹,

Falkler²

1983

J Clin Microbiol

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Antisera were produced which were reactive with Bacteroides fragilis, B. gingjralis, or B. melaninogenicus subsp. melaninogenicus. Anti-B. gingivalis seruli exhibited strong reactions with strains of homologous species and reacted with only one other species, B. thetaiotaomicron. Antigens shared by organisms of the B. fragilis group and B. melaninogenicus subspecies of the Bacteroidaceae must be considered when serological methods are used for their identification.

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Section: Discussionsupporting

confidence: 49%

Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Shaefer¹,

Falkler²

1983

J Clin Microbiol

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“…Several attempts have been made, mostly by isolating the organism from an established infection and using it as a source of the antigen (eg, Danielsson et al, 1972), or by using type cultures of the Bacteroidaceae as sources of antigen (eg, Quick et al, 1972;Rissing et al, 1974). Many negative results were obtained from cases of proven infections involving identified Bacteroides species.…”

mentioning

confidence: 99%

Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay.

Poxton¹

1979

J Clin Pathol

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SUMMARY An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. Cross-reactivity between heterologous species and antiserum was only significant between closely related biotypes. This cross-reactivity could be removed by absorption of the antisera with whole cells. Tabaqchali, 1978a).There is much interest in the possible use of serological techniques for the titration of serum antibodies to establish the involvement of a Bacteroides species in an infection, or to show the normal levels of antibodies directed against commensal strains of Bacteroides in healthy or compromised individuals. Several attempts have been made, mostly by isolating the organism from an established infection and using it as a source of the antigen (eg, Danielsson et al., 1972), or by using type cultures of the Bacteroidaceae as sources of antigen (eg, Quick et al., 1972;Rissing et al., 1974). Many negative results were obtained from cases of proven infections involving identified Bacteroides species.These studies all suffer from a basic lack of knowledge of the immunogenic structures of the

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“…Small amounts of natural antibodies, mainly IgM, are found in normal human serum using highly sensitive methods such as haemagglutination and immunofluorescent tests (Danielsson et al, 1972;Quick et al, 1972;Rissing et al, 1974). The antibody detected by the CIEP test is mainly IgG (Lightfoot, 1976), and our findings indicate that it can detect specific B. fragilis antibodies in the serum of patients with active infection.…”

Section: Discussionmentioning

confidence: 64%

“…In these infections the pathogenic role of B. fragilis can be ascertained either by a good response to treatment with metronidazole or by the finding of specific antibodies in the patient's serum. Recent reports indicate that antibodies can be demonstrated in sera by agglutination, haemagglutination, immunodiffusion, and immunofluorescent methods (Danielsson et al, 1972;Quick et al, 1972;Rissing et al, 1974). The reports show that IgG immunoglobulins are probably more important than IgM in the diagnosis of bacteroides infection.…”

mentioning

confidence: 99%

Diagnosis of Bacteroides fragilis infection with counter-immunoelectrophoresis.

Okubadejo¹,

Lightfoot²,

Hewitt³

1978

J Clin Pathol

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SUMMARY In a study of 188 patients and 109 controls, the detection of antibody by counterimmunoelectrophoresis was used as a diagnostic aid in human infections with Bacteroides fragilis. It was found that positive results indicated current infection and negative results were not conclusive. The method used was simple, rapid, and easily performed in a routine laboratory, but further work is needed to enhance antigen potency.Bacteroides fragilis strains are often cultured from routine bacteriological specimens in laboratories with adequate anaerobic technique (Leigh, 1974; Peach, 1975). At present the organisms are the most frequent cause of non-sporing Gram-negative anaerobic infection in man. During the past five years the bacteria have been isolated with increasing frequency in many laboratories. Nevertheless the pathogenicity of the isolates is often questioned because the bacteria are a major component of normal body flora (Quick, 1972;Topley, 1975;Willis, 1975). A foul-smelling discharge usually indicates anaerobic infection, butthis sign may be absent in certain lesions in which B. fragilis is a likely infective agent. These include superficial wounds, bed sores, diabetic ulcers, salpingitis, pelvic inflammatory disease, vaginitis, episiotomy wounds, and otitis. In these infections the pathogenic role of B. fragilis can be ascertained either by a good response to treatment with metronidazole or by the finding of specific antibodies in the patient's serum. Recent reports indicate that antibodies can be demonstrated in sera by agglutination, haemagglutination, immunodiffusion, and immunofluorescent methods (Danielsson et al., 1972;Quick et al., 1972;Rissing et al., 1974). The reports show that IgG immunoglobulins are probably more important than IgM in the diagnosis of bacteroides infection. IgG antibodies can be detected efficiently and quickly by counter-immunoelectrophoresis (World Health Organisation, 1973); so far as we know, this technique has not been applied to the detection of antibodies in B. fragilis. We report a study using this method."Surgeon Lieutenant Commander, Royal Navy Received for publication 29 March 1978 Patients and methods INFECTED PATIENTS' TEST SERA Sera were taken from patients suspected of having bacteroides infection because of positive cultures or persistent, foul-smelling, purulent lesions suggesting anaerobic infection. Initial specimens of serum were usually obtained within three days of the isolation of B. fragilis, and subsequent serum samples were taken whenever possible at an interval of about 7-10 days.CONTROL SERA Sera were also collected from patients who had no evidence of infection but had had B. fragilis in high vaginal swabs before a gynaecological operation (uninfected patients). Other control sera were obtained from healthy young male naval recruits and healthy female blood donors.All sera used in this study were taken from blood given for other purposes. COUNTER-IMMUNOELECTROPHORESIS (CIEP)Tests were performed using Shandon Southern U77 electrophoretic appar...

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Detection of Bacteroides fragilis Infection by Precipitin Antibody

Cited by 26 publications

References 8 publications

Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay.

Diagnosis of Bacteroides fragilis infection with counter-immunoelectrophoresis.

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