Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Shaefer, D F; Falkler, William A.

doi:10.1128/jcm.18.1.18-22.1983

J Clin Microbiol

1983

DOI: 10.1128/jcm.18.1.18-22.1983

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Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

D F Shaefer¹,

William A. Falkler²

Abstract: Antisera were produced which were reactive with Bacteroides fragilis, B. gingjralis, or B. melaninogenicus subsp. melaninogenicus. Anti-B. gingivalis seruli exhibited strong reactions with strains of homologous species and reacted with only one other species, B. thetaiotaomicron. Antigens shared by organisms of the B. fragilis group and B. melaninogenicus subspecies of the Bacteroidaceae must be considered when serological methods are used for their identification.

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Cited by 4 publications

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“…originally established B. gingivalis as distinct from B. asaccharolyticus. Polyclonal reagents have also been used to examine antigenic heterogeneity in B. gingivalis (13,17,18). Reed et al (17) and Mouton et al (13) found that these reagents were specific for B. gingivalis; however, the presence of a cross-reactive antigen in strains of Bacteroidaceae has been reported (10).…”

mentioning

confidence: 99%

The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples

Chen¹,

Bochacki²,

Reynolds³

et al. 1986

Infect Immun

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Hybridomas were established which produce monoclonal antibodies specific for Bacteroides gingivalis, a pathogen associated with human periodontal disease. Spleen cells from BALB/c mice immunized with formalinized B. gingivalis were fused with Sp2/0-Ag14 myeloma cells. Of 1,050 wells with positive growth, 60 contained antibody reactive with the immunizing strain of B. gingivalis by enzyme-linked immunosorbent assay. Expansion of these cultures and cloning by limited dilution resulted in 28 clones which reacted with B. gingivalis but not with other orals and nonoral black-pigmented Bacteroides species or any of 29 representative strains of other oral bacteria. Of these 28 clones, 14 were also specific for B. gingivalis by indirect immunofluorescence microscopy. One clone, BBG-12 producing immunoglobulin G2b(kappa), was chosen to identify B. gingivalis in subgingival plaque because of its high reactivity in indirect immunofluorescence assays. This antibody reacted strongly with all 17 representative B. gingivalis strains obtained from diverse sources. Furthermore, when this reagent was applied to subgingival plaque samples, B. gingivalis was stained with high specificity and low background fluorescence, indicating that it may be useful for clinical identification of this organism.

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mentioning

confidence: 99%

The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples

Chen¹,

Bochacki²,

Reynolds³

et al. 1986

Infect Immun

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Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species

Ogawa¹,

Mukai²,

Shimauchi³

et al. 1991

Oral Microbiology and Immunology

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Rabbit polyclonal antibody (Poly-1) and mouse monoclonal antibodies (mAbs) TO-11, TO-14 and TO-M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly-1 and the 3 mAbs were screened for their reactivity with whole cells oral and nonoral black-pigmented bacterial species by enzyme-linked immunosorbent assay (ELISA) and the binding experiment using [125I]Poly-1 and [125I]mAbs. ELISA revealed that Poly-1 definitely reacted with whole cells of all the 11 strains of P. gingivalis tested. However, 8 of 11 P. gingivalis strains reacted with mAbs TO-11, TO-14 and TO-M1. These results were confirmed by the specific binding of radiolabelled Poly-1 and mAb TO-11 to the 8 strains. The M(r) of the fimbrial subunit protein (fimbrilin) isolated and purified from P. gingivalis strains 381, BH18/10, HW24D-1, 6/26 and OMZ 314 was 41 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It was found by immunoblotting that mAbs TO-11 and TO-14/TO-M1 recognized different epitopes of fimbrial protein from P. gingivalis strains. Immunoelectron micrographs of whole cells and the purified fimbriae of P. gingivalis strains visualized similar serotype-specific antibody bindings to the fimbriae. These results indicate that 11 strains of P. gingivalis could be divided into at least 2 separate groups based on the immunochemical specificities of the fimbriae.

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Serological classification of Bacteroides from the human oral cavity

Ebersole

Taubman

Smith

et al. 1988

J of Periodontal Research

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Ebersole JL, Taubman MA. Smith DJ, Frey DE: Serological classification of Bacteroides//-om the human oral cavity. J Periodont Res 1988: 23: 22-27. Studies were initiated to investigate the antigenic relationship among orai species of Bacteroides. Antigenic differences among the Bacteroides were assessed with an ELISA using antisera from immunized rabbits and sera obtained from subjects with periodontal disease. Multiple species of Bacleroides were examined, including; B. gingivalis. 2 homology groups of B. inlermedius, B. melaninogenieus, B. denticola. B. loescheii, B. oralis. B. ievii. B. gracilis. B. huccae. and B. eorporis. Both rabbit and human sera reacted with distinct surface antigenic determinants in each of the SF>ecies oi Bacteroides. Additional relationships were defined, such that the 2 homology groups of B. intermedius exhibited a similarity of approximately 30-40%, while the B. melaninogenicus and B. loescheii showed a 20-25% similarity of surface antigens. B. denticola was antigenically related to the B. intermedius and B. melaninogenicus strains by 10-15%, The remaining cross-reactivities were generally less than 5% among the Bacteroides species. The findings are consistent with specific surface antigenic determinants on the oral Bacteroides spp. and suggest the ability to detect specific responses to these organisms in periodontal disease patients.

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Reaction of clinical isolates and typed strains of the family Bacteroidaceae with rabbit anti-Bacteroides sera examined by radial immunodiffusion

Cited by 4 publications

References 23 publications

The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples

The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples

Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species

Serological classification of Bacteroides from the human oral cavity

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