Serological classification of <i>Bacteroides</i> from the human oral cavity

Ebersole, Jeffrey L.; Taubman, Martin A.; Smith, Daniel J.; Frey, D. E.

doi:10.1111/j.1600-0765.1988.tb01022.x

Cited by 8 publications

(3 citation statements)

References 32 publications

(13 reference statements)

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“…It has been shown that various periodontal species contain strains with different virulence (van Steenbergen etal, 1982;Sundqvist et al, 1982;Neiders et al, 1989). Further, serotype b of A. actinomycetemcomitans and serotype I of P. intermedia are more associated with periodontal disease (Zambon et al, 1983;Ebersole et al, 1988;Dahlen et al, 1990a). Since virulence factors are phenotypical expressions of the genotype, it is suggested that variation in virulence may be related to certain genotypes or that virulence factors may be Pos.…”

Section: Enzyme-activitymentioning

confidence: 95%

Role of Suspected Periodontopathogens in Microbiological Monitoring of Periodontitis

Dahlén

1993

Adv Dent Res.

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Periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. Although a specificity to some degree is found for Actinobacillus actinomycetemcomitans in localized juvenile periodontitis (LJP), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. What we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshold of tissue integrity. This hypothesis is concomitant with the view of periodontal disease as a polymicrobial infection, predominantly anaerobic, which occurs commonly in the oral cavity or elsewhere in the body. Some micro-organisms (risk markers) occur more frequently than others and may significantly determine the outcome of this host-parasite interaction. Microbiological sampling and analysis seem to be of limited value in risk assessment; however, they can be used as tools in diagnosis in LJP patients and acute infections, and in treatment decision and therapy control in "refractory" patients. Suspected pathogens (risk markers) are Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and some species of spirochetes, while the roles of Prevotella intermedia, Bacteroidesforsythus, Fusobacterium nucleatum, Campylobacter rectus, and Peptostreptococcus micros are more uncertain. The presence of periodontopathogens as well as enterics, Staphylococcus aureus and Candida especially, should be considered in patients with systemic individual disorders--e.g., diabetes mellitus, neutropenia, agranulocytosis, and AIDS--or with implants.

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Section: Enzyme-activitymentioning

confidence: 95%

Role of Suspected Periodontopathogens in Microbiological Monitoring of Periodontitis

Dahlén

1993

Adv Dent Res.

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“…The cells were harvested by centrifugation (7,000 x g), and the pellets were washed with sterile PBS (0.02 M phosphate, pH 7.4). The cells were formalin killed by overnight treatment with 0.5% buffered formal saline (13). The killed W. recta cells were washed and stored at 4°C in PBS.…”

Section: Methodsmentioning

confidence: 99%

Virulence of Wolinella recta in a murine abscess model

et al. 1991

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The virulence of Wolinella recta isolates was studied in an experimental animal model by using monoinfection of BALB/c mice. Infection with clinical isolates of W. recta 576 and W. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas W. recta ATCC 33238 failed to induce a similar lesion. Histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum proteins and neutrophils. Virulence-modulating agents such as dexamethasone, galactosamine, hydrazine sulfate, and dextran microcarrier beads were used in conjunction with W. recta infection. Dexamethasone, hydrazine sulfate, and dextran beads enhanced the infectivity and pathogenicity of W. recta for lesion formation and tissue destruction compared with what was found in untreated control mice. Galactosamine sensitization enhanced the virulence potential of W. recta to such an extent that a lethal outcome was observed. Laboratory passage of clinical isolates demonstrated a decreased virulence in high-passage strains, which correlated with the minimal virulence observed in the extensively passaged W. recta ATCC 33238. Serum immunoglobulin G (IgG) and IgM responses were detected in the serum of infected animals, and cross-reacting antibody indicated variation in the antigenic makeup of various W. recta strains. Enhanced IgG antibody responses were observed following the secondary challenge. Mice with acquired antibody response to initial infection remained susceptible to lesion formation with subsequent challenge, but the size of the lesion was significantly reduced, indicating partial protection. Serum IgG and IgM antibody levels were significantly increased by active immunization when compared with levels in mice which had recovered from infection. The immunization significantly decreased the lesion size; however, even these high levels of antibody failed to abrogate the lesion induction. 2806 on August 5, 2020 by guest

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“…Reports by Ebersole et al have also described the potential usefulness of the enzyme-linked immunosorbent assay (ELISA) in the serologic identification of oral Bacteroides spp. and other microorganisms (5,6).…”

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confidence: 99%

Slot immunoblot assay for detection and quantitation of periodontal disease-associated microorganisms in dental plaque

Poperin

Lopatin

1991

J Clin Microbiol

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A rapid method for qualitative and quantitative detection of specific oral microorganisms from subgingival dental plaque is described. Plaque samples were suspended in phosphate-buffered saline containing protease inhibitors and 0.5% formaldehyde, briefly sonicated to disperse bacterial aggregates, and applied to nitrocellulose membranes in a slot blot manifold. Subsequent incubations with species-specific rabbit antibody and anti-rabbit antibody-alkaline phosphatase conjugate and development with BCIP-NBT substrate resulted in an easily discernible, permanent stain being deposited at the sample application site. Comparison with known concentrations of pure, cultured microorganisms applied to the same membranes permitted qualitative or semiquantitative plaque characterization by visual inspection. Analysis of the blots with a computer-linked flatbed scanner provided quantitative data on microbial content. The reproducibility of the results (standard error of the mean, <10%) obtained with slot immunoblotting greatly exceeded that of the results obtained with immunofluorescence analysis (standard error of the mean, >57%). Because it is versatile, rapid, sensitive, reproducible, permanent, and relatively inexpensive, slot immunoblotting lends itself to use in large-scale investigations for the detection and quantitation of specific microbial species.

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Serological classification of Bacteroides from the human oral cavity

Cited by 8 publications

References 32 publications

Role of Suspected Periodontopathogens in Microbiological Monitoring of Periodontitis

Role of Suspected Periodontopathogens in Microbiological Monitoring of Periodontitis

Virulence of Wolinella recta in a murine abscess model

Slot immunoblot assay for detection and quantitation of periodontal disease-associated microorganisms in dental plaque

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