Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay.

Abstract

SUMMARY An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the heterologous type species. Cross-reactivity between heterologous species and antiserum was only significant between closely related biotypes. This cross-reactivity could be removed by absorption of the antisera with whole cells. Tabaqchali, 1978a).There is much interest in the possible use of serological techniques for the titration of serum antibodies to establish the involvement of a Bacteroides species in an infection, or to show the normal levels of antibodies directed against commensal strains of Bacteroides in healthy or compromised individuals. Several attempts have been made, mostly by isolating the organism from an established infection and using it as a source of the antigen (eg, Danielsson et al., 1972), or by using type cultures of the Bacteroidaceae as sources of antigen (eg, Quick et al., 1972;Rissing et al., 1974). Many negative results were obtained from cases of proven infections involving identified Bacteroides species.These studies all suffer from a basic lack of knowledge of the immunogenic structures of the

Detection of Gonococcal Antigens by an Indirect Sandwich Enzyme-linked Immunosorbent Assay

“…These results confirm the evidence presented by other investigators for adequate binding of protein to polystyrene (Brodeur, Ashton and Diena, 1978;Glynn and Ison, 1978;Poxton, 1979;Young and Low, 1981). With homologous OM complex, the antibody titres of the anti-strain 9 and anti-strain 82409 rabbit serum were 15.3 and 12.2 times higher than when determined by GCFT.…”

Analysis of EDTA-soluble Cell Surface Components of Gram-positive Anaerobic Cocci

“…Four clinical isolates of GPAC were selected for the production of antisera in rabbits, based on the method of Poxton (1979). The strains were Ps.…”

A Novel Enzyme Immunoassay Commonly Applied for Ten Strains of Pyricularia oryzae

“…Ind., Osaka, Japan. /3-D-Galactosidase (GAL)-labeled goat anti-rabbit IgG antibody (19) Buffers. PBS, 0.01 M phosphate-buffered saline, pH 7.0; buffer A (0.02 M sodium phosphate buffer, pH 7.0, containing 0.1 M NaC1, 1 mm MgC12, 0.1 % BSA and 0.1 % NaN3) ; buffer B (0.05 M sodium phosphate, pH 7.4, containing 0.01 M EDTA and 0.1% BSA).…”