Analysis of EDTA-soluble Cell Surface Components of Gram-positive Anaerobic Cocci

Abstract

The protein content of EDTA extracts from 76 strains of Gram-positive anaerobic cocci was examined using SDS-PAGE. Strains of Peptostreptococcus anaerobius produced almost identical profiles ; greater heterogeneity was observed within the species Peptococcus rnagnus, Peptococcus prevotii and Peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. Serological investigation of these extracts by ELISA revealed numerous crossreactions among the different biotypes. Immunoblot transfers from polyacrylamide gels demonstrated two common antigens within strains of the species Ps. anaerobius, but these were not species-specific.

The differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis

“…We concluded, as did Smith et al, 23 that, on the basis of protein patterns, P . magnus, as currently defined, is heterogeneous.…”

“…Our isolates of "P. prevotii" came from various sites, including the vagina. Smith et al 23 found that their P . prevotii was heterogeneous but, as we did originally, could have included indole-negative P .…”

Ex vivo killing of Enterococcus faecalis and mixed plaque bacteria in planktonic and biofilm culture by modified photoactivated disinfection

“…It has also been shown that EDTA can remove some surface components of gram‐positive E. faecalis (Hancock , Smith et al . , George et al . ).…”

Crystalline surface protein of Peptostreptococcus anaerobius

“…Peptostreptococcus strains were obtained from reference culture collections (DSM, German Collection of Micro-organisms ; ATCC, American Type Culture Collection) or isolated from infections of the oral cavity (Table 1). Identification of the clinical strains was based on Gram staining, oxygen tolerance, profiles of preformed enzymes (RapID ANA I1 system, Innovative Diagnostic Systems ; and API AnIdent system, API Analytab Products) and comparison of the wholecell protein profiles with the reference strains in SDS-PAGE (see below) (Smith et al, 1986b). Pure cultures were preserved in skim milk (Difco) at -70 OC.…”