Detection of avian group D rotavirus using the polymerase chain reaction for the VP6 gene

Bezerra, Delana Andreza Melo; Silva, René Ribeiro da; Kaiano, Jane Haruko Lima; Silvestre, Rodrigo Vellasco Duarte; Oliveira, Darleise de Souza; Linhares, Alexandre C.; Gabbay, Yvone Benchimol; Mascarenhas, Joana D’Arc Pereira

doi:10.1016/j.jviromet.2012.07.017

Cited by 30 publications

(46 citation statements)

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“…The nucleotide comparison of the strains from this study with RVD previously described in Brazil revealed a high identity (99% nucleotide identity) among KJ101589 (Bezerra et al 2012, Bezerra et al 2014) and KC689307. Likewise, the putative amino acids sequences all samples showed 100% identity, except the strain KC689306 (99.5-99.9% amino acid identity) (Bezerra et al 2012, Bezerra et al 2014.…”

Section: Sequencing Analysis Of the Vp6 Genesupporting

confidence: 55%

“…Likewise, the putative amino acids sequences all samples showed 100% identity, except the strain KC689306 (99.5-99.9% amino acid identity) (Bezerra et al 2012, Bezerra et al 2014.…”

Section: Sequencing Analysis Of the Vp6 Genementioning

confidence: 90%

“…In Brazil, RVD was previously detected in Northern Brazilian Region by a specific RT-PCR assay, with a high occurrence ranging from 53% (16/30 samples) to 35.3% (30/85) (Bezerra et al 2012, Bezerra et al 2014.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the avian rotaviruses detected in Brazil belong to RVA , Asano et al 2011. Nevertheless, RVD and RVF have been recently described in the country (Bezerra et al 2012, Bezerra et al 2014). Here we report the occurrence of RVD in feces of broilers, layers and broiler breeders on Brazilian poultry farms representing 8 different states, using a RT-PCR technique based on the amplification of the VP6 gene, followed by nucleotide sequencing reaction of the amplicons in order to better understand the phylogeny of the strains detected.…”

Section: Introductionmentioning

confidence: 99%

“…Total RNA was extracted with TRIzol Reagent ® (Invitrogen™) and cDNA was synthesized using random primers (Invitrogen™) and M-MLV Reverse Transcriptase (Invitrogen™) as described by the manufacturer. The cDNA was subjected to PCR targeting the VP6 gene, with RVD specific primers and thermal cycling conditions described by Bezerra et al (2012) generating a 742 bp gel band.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

et al. 2015

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RESUMO.-[Monitoramento e caracterização molecular de rotavírus do grupo D em criações comerciais brasileiras.] Rotavírus são agentes etiológicos de diarreia tanto em humanos como em várias espécies animais. Dados sobre rotavírus do grupo D (RVD) em aves são escassos, especialmente no Brasil. Nós detectamos RVD em 4 pools de conteúdo intestinal de frango de corte, poedeiras e matrizes de um total de 111 pools originários de 8 estados brasileiros, representando uma ocorrência de 3,6% a partir de uma RT-PCR específica para RVD, tendo como alvo o gene VP6. A árvore filogenética confirmou que as amostras brasileiras pertencem ao grupo D e três das sequências obtidas foram idênticas em termos de aminoácidos enquanto uma apresentou 99,5% de identidade com as demais. As sequências aqui definidas são semelhantes a outras sequências previamente definidas no Brasil, confirmando a natureza conservada da proteína VP6. INTRODUCTIONRotaviruses are members of the Reoviridae family, and they are a major cause of acute gastroenteritis in young children and several mammalian and avian species (Brüssow et al. 1992, Estes & Kapikian 2007. They are non-enveloped icosahedral particles and its genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1 to VP4, VP6 and VP7) and six nonstructural proteins (NSP1 to NSP6). Based on antibody reactivity or genetic sequencing of structural protein VP6 of the internal capsid, rotaviruses have been classified into the groups A to G ( Brazil. E-mail: laila_andreia@hotmail.com Rotaviruses are etiological agents of diarrhea both in humans and in several animal species. Data on avian Group D rotaviruses (RVD) are scarce, especially in Brazil. We detected RVD in 4 pools of intestinal contents of broilers, layer and broiler breeders out of a total of 111 pools from 8 Brazilian states, representing an occurrence of 3.6%, by a specific RVD RT-PCR targeting the VP6 gene. Phylogenetic tree confirmed that the Brazilian strains belong to group D and 3 of the sequences were identical in terms of amino acid whereas one showed 99.5% identity with the others. The sequences described in this study are similar to other sequences previously detected in Brazil, confirming the conserved nature of the VP6 protein.

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Section: Sequencing Analysis Of the Vp6 Genesupporting

confidence: 55%

“…Likewise, the putative amino acids sequences all samples showed 100% identity, except the strain KC689306 (99.5-99.9% amino acid identity) (Bezerra et al 2012, Bezerra et al 2014.…”

Section: Sequencing Analysis Of the Vp6 Genementioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

et al. 2015

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Animal Rotaviruses

Vlasova

Deol

Sircar

et al. 2020

Livestock Diseases and Management

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Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

et al. 2016

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The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.

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Detection of avian group D rotavirus using the polymerase chain reaction for the VP6 gene

Cited by 30 publications

References 20 publications

Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

Monitoring and molecular characterization of group Drotavirus in Brazilian poultry farms

Animal Rotaviruses

Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil

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