Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

Axpe, Eneko; Lopez-Euba, Tamara; Castellanos–Rubio, Ainara; Mérida, David; Garcı́a, J.A.; Plaza-Izurieta, Leticia; Fernández-Jiménez, Nora; Plazaola, F.; Bilbao, José Ramón

doi:10.1371/journal.pone.0083838

Cited by 38 publications

(36 citation statements)

References 15 publications

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“…This PT-100 and the heater were installed in an aluminium sample holder. The manufacturing of the 22 NaCl sealed source of 15 µCi was described in detail in a previous article 27 . The positron source was sandwiched between two identical samples.…”

Section: Positron Annihilation Lifetime Spectroscopy (Pals)mentioning

confidence: 99%

Ceramide increases free volume voids in DPPC membranes

et al. 2015

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Positron annihilation lifetime spectroscopy (PALS) can measure changes in local free volume voids in lipid bilayers. PALS has been applied, together with differential scanning calorimetry (DSC) and molecular dynamics (MD) simulations, to study free volume voids in DPPC and DPPC:ceramide (85:15 mol:mol) model membranes in the 20-60 ºC range. The free volume void average size clearly increases with the gel-fluid phase transition of the lipid, or lipid mixture. Ceramide increases void size at all temperatures, particularly in the range causing the gel-fluid transition of the mixture. A parallel study of PALS and calorimetric data indicates that, for the complex thermotropic transition of the DPPC:ceramide mixture, PALS is detecting the transition of the DPPC component, while calorimetry changes indicate mainly the melting of the ceramide-enriched domains. Molecular dynamics calculations provide a clear distinction between ceramide-rich and -poor domains, and show that the voids are predominantly located near the membrane nodal plane. The ceramide-induced increase in void volume size occurs as well at temperatures when both phospholipid and ceramide are in the fluid state, indicating that the effect is not the result of phospholipid-ceramide domain coexistence. The above observations may be related to hitherto unexplained properties of ceramide, such as the increase in membrane permeability, and the induction of transmembrane (flip-flop) lipid motion.

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Section: Positron Annihilation Lifetime Spectroscopy (Pals)mentioning

confidence: 99%

Ceramide increases free volume voids in DPPC membranes

et al. 2015

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“…simultaneous imaging of the density distribution of annihilation points as well as positron annihilation lifetime spectroscopy [8,36]. The positronium imaging technique has successfully distinguished cancer from healthy tissue by in vitro probing of human tissues or in the in vitro organoid (3D cell culture) systems [37]. Study of positronium decay can provide new data in medical diagnosis as the mean lifetime of positronium depends on the size of free volume between atoms, whereas its formation probability depends on the concentration of voids [38].…”

Section: Concept Of J-pemmentioning

confidence: 99%

Development of J-PEM for Breast Cancer Detection and Diagnosis Using Positronium Imaging

Shivani¹,

Luczynska²,

Heinze³

et al. 2020

Acta Phys. Pol. B

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The purpose of the presented investigations is to design, construct and establish the characteristic performance of the Jagiellonian Positron Emission Mammography(J-PEM), being designed for the detection and diagnosis of breast cancer. Its construction is based on a novel idea of PET tomography based on plastic scintillators and wavelength shifter (WLS) and a new concept of positronium imaging. We have prepared a simulation program based on Monte Carlo methods for optimizing the geometry and material of the J-PEM prototype. Here we present the first results from the simulations and a brief review of the state of art of breast imaging modalities and their characteristics motivating our investigation.

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“…PALS techniques were applied successfully to investigate some living biological systems [25][26][27][28]. As it was mentioned above both factors: void sizes and concentration influence the 3γ fraction.…”

Section: Applications To Medical Imagingmentioning

confidence: 99%

New Porosimetric Method Based on the 3γ Annihilation Rate. Applications to Materials Science and Medical Imaging

Jasińska

Zgardzińska

2017

Acta Phys. Pol. A

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A new parameter δ based on 3γ annihilation is proposed. It describes the porosity of the materials containing small intermolecular voids below 1 nm. It can be applied to material investigation to compare changes in the material porosity depending on temperature or pressure as well as to study of ageing or manufacturing processes. Particularly it is dedicated to prepare the new imaging method and can be used during positron emission tomography diagnosis allowing determination the kind or stage of pathogenic alteration.

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Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

Cited by 38 publications

References 15 publications

Ceramide increases free volume voids in DPPC membranes

Ceramide increases free volume voids in DPPC membranes

Development of J-PEM for Breast Cancer Detection and Diagnosis Using Positronium Imaging

New Porosimetric Method Based on the 3γ Annihilation Rate. Applications to Materials Science and Medical Imaging

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