Detection of Antibiotics Residue in Chicken Meat 
 Using TLC

., M.A. Tajick; Shohreh, Bahram

doi:10.3923/ijps.2006.611.612

Cited by 38 publications

(3 citation statements)

References 8 publications

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“…The upper oily layer was discarded and defatation of bottom layer was further done twice with equal volumes of diethyl ether. It has been found that concentrating the extract made detection easier while performing TLC (Tajick & Shohreh, 2006). So the defatted extract was then concentrated to about 2 mL in a rotary vacuum evaporator at a temp of 50 °C.…”

Section: Sample Preparationmentioning

confidence: 99%

Detection of Antibiotic Residues in Broiler Meat by Thin Layer Chromatography

Thapa¹,

Acharya

Dahal³

et al. 2023

KMC J.

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Antibiotic usage has played a significant role in the growth of poultry industry all across the globe. But because of indiscriminate use of antibiotics, several incidences related to occurrence of antibiotic residues in broiler meat have been reported. In the context of Nepal, only limited studies have been attempted to assess the presence of antibiotic residues in poultry meat and thus this study aims to determine the same in Dharan. A questionnaire survey was conducted among poultry farmers and veterinary shops in Dharan where 44% of respondents had training on poultry farming but only 4 % had knowledge on antimicrobial resistance in microorganism. The survey report showed maximum usage of doxycycline and tetracycline in poultry farms. Four types of broiler meat, namely, liver, breast muscle, kidney and gizzard were collected and screening of antibiotic residues (ciprofloxacin, doxycycline, enrofloxacin, gentamycin and tetracycline) in them was performed by thin layer chromatography. On thin layer chromatography, 9%, 17%, 8%, 3% and 21% of samples were detected with ciprofloxacin, doxycycline, enrofloxacin, gentamycin and tetracycline residues respectively. Highest occurrence of ciprofloxacin, doxycycline, enrofloxacin and tetracycline residues were found in kidney (16%), gizzard (32%), liver (12%) and kidney (36%) samples respectively.

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Section: Sample Preparationmentioning

confidence: 99%

Detection of Antibiotic Residues in Broiler Meat by Thin Layer Chromatography

Thapa¹,

Acharya

Dahal³

et al. 2023

KMC J.

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show abstract

“…Chromatography TLC was performed according to [8] and [14] with some modifications. After cutting TLC plate (MN-Germany) into appropriate size (4x5 cm), two straight lines were drawn across the plate approximately 2cm from the bottom and 1 cm from the upper edge with a pencil.…”

Section: G Detection Of Antibiotic Residue By Thin Layermentioning

confidence: 99%

Detection of Heavy Metals and Antibiotic Residues in Poultry Feeds in Selected Areas of Bangladesh

et al. 2023

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Presence of heavy metals and antibiotics in the poultry feed even in low concentrations is considered as potentially toxic and may cause hazardous effects on animal leading to accumulate in food chain. This study was performed to assess the status of heavy metals and antibiotics residue in different poultry feed samples collected from local markets of Mymensingh and Kishoreganj sadar upazila of Bangladesh. A total of 40 poultry feed samples were analyzed to detect the concentration of three heavy metals- chromium (Cr), cadmium (Cd) and lead (Pb) using atomic absorption spectrophotometry and antibiotic residues using thin layer chromatography. A survey was also conducted among 30 feed sellers in the study areas whose knowledge regarding presence of heavy metals and antibiotics residues in poultry feed was evaluated. Survey data showed that about 80% feed sellers were literate whereas 20% were illiterate. Around 23% feed sellers had knowledge about the presence of heavy metals and 90% had about antibiotic residues in poultry feeds and its detrimental public health effects. The average Cr, Cd and Pb contents in poultry feed collected from Mymensingh sadar upazila were 0.402, 1.630 and 18.314 mgkg-1, respectively. The average content of Cr and Cd in feed collected from Kishoreganj sadar upazila were 7.884 and 0.006 mgkg-1, respectively, whereas Pb content was below the detection level in this area. The results from this study showed that Cd and Pb in Mymensingh sadar upazila, Cr in Kishoreganj sadar upazila, ciprofloxacin, doxycycline and oxytetracycline in both upazilas were present at alarming levels in most of the feed samples which could be harmful for poultry as well as human health. Therefore, it is suggested to take proper steps for monitoring and regular detection of heavy metals and antibiotic residues in poultry feeds with sources identification as we as community-based awareness among the stakeholder.

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“…Residues are defined as chemical substances or metabolites of medicinal products that may accumulate within the tissues or edible parts of treated animals (EC-European Commission, 2012). These residues may result from inappropriate or extra-label drug usage, failure to maintain drug withdrawal periods, or poor livestock production practices (Tajick and Shohreh, 2006). The treated animals may rapidly and efficiently metabolize some drugs while slowly and poorly metabolizing others; thus, the residues accumulate in the edible portion of the animals.…”

Section: Introductionmentioning

confidence: 99%

Reducing Veterinary Drug Residues in Animal Products: A Review

Rana

Lee

Kang

2019

Food Sci Anim Resour

109

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A survey we conducted suggests that the ingestion of veterinary drug residues in edible animal parts constitutes a potential health hazard for its consumers, including, specifically, the possibility of developing multidrug resistance, carcinogenicity, and disruption of intestinal normal microflora. The survey results indicated that antibiotics, parasitic drugs, anticoccidial, or nonsteroidal anti-inflammatory drugs (NSAIDs) are broadly used, and this use in livestock is associated with the appearance of residues in various animal products such as milk, meat, and eggs. We observed that different cooking procedures, heating temperatures, storage times, fermentation, and pH have the potential to decrease drug residues in animal products. Several studies have reported the use of thermal treatments and sterilization to decrease the quantity of antibiotics such as tetracycline, oxytetracycline, macrolides, and sulfonamides, in animal products. Fermentation treatments also decreased levels of penicillin and pesticides such as dimethoate, malathion, Dichlorodiphenyldichloroethylene, and lindane. pH, known to influence decreases in cloxacillin and oxacillin levels, reportedly enhanced the dissolution of antimicrobial drug residues. Pressure cooking also reduced aldrin, dieldrin, and endosulfan in animal products. Therefore, this review provides updated information on the control of drug residues in animal products, which is of significance to veterinarians, livestock producers, and consumer health.

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Detection of Antibiotics Residue in Chicken Meat Using TLC

Cited by 38 publications

References 8 publications

Detection of Antibiotic Residues in Broiler Meat by Thin Layer Chromatography

Detection of Antibiotic Residues in Broiler Meat by Thin Layer Chromatography

Detection of Heavy Metals and Antibiotic Residues in Poultry Feeds in Selected Areas of Bangladesh

Reducing Veterinary Drug Residues in Animal Products: A Review

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