Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

Affonso, Ingrid Bortolin; Souza, Andressa de; Martini, Matheus Cavalheiro; Santos, Márcia Mercês Aparecida Bianchi dos; Spilki, Fernando Rosado; Arns, Clarice Weis; Samara, Samir Issa

doi:10.5433/1679-0359.2014v35n5p2539

Cited by 5 publications

(3 citation statements)

References 17 publications

(37 reference statements)

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“…1c ) differ between human and bovine RSV F (N63K and Q202E). The other main difference between the two RSV F molecules is the presence of an N-linked glycosylation site at N70 in hRSVF that is absent in the bovine counterpart for the strain used in this study 37 (Figs. 1a, c ).…”

Section: Resultsmentioning

confidence: 99%

Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV

et al. 2017

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Human respiratory syncytial virus (hRSV) is responsible for serious lower respiratory tract disease in infants and in older adults, and remains an important vaccine need. RSV fusion (F) glycoprotein is a key target for neutralizing antibodies. RSV F stabilized in its pre-fusion conformation (DS-Cav1 F) induces high neutralizing antibody titers in naïve animals, but it remains unknown to what extent pre-fusion F can boost pre-existing neutralizing responses in RSV seropositive adults. We here assess DS-Cav1 F immunogenicity in seropositive cattle pre-exposed to bovine RSV, a virus closely related to hRSV. A single immunization with non-adjuvanted DS-Cav1 F strongly boosts RSV neutralizing responses, directed towards pre-fusion F-specific epitopes, whereas a post-fusion F is unable to do so. Vaccination with pre-fusion F thus represents a promising strategy for maternal immunization and for other RSV vaccine target populations such as older adults.

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Section: Resultsmentioning

confidence: 99%

Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV

et al. 2017

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“…Following a positive ELISA, lung homogenates were added to the cell culture and observed daily until CPE including syncytia and round apoptotic cells were visible. The isolation of BRSV in culture can be challenging because the virus is relatively labile and can struggle to grow in culture; however, this successful isolation now opens the door to extensive studies that would not have been possible otherwise [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Circulation of Indigenous Bovine Respiratory Syncytial Virus Strains in Turkish Cattle: The First Isolation and Molecular Characterization

Yazıcı

Özan

Tamer

et al. 2020

Animals

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Bovine respiratory disease (BRD) is a huge economic burden on the livestock industries of countries worldwide. Bovine respiratory syncytial virus (BRSV) is one of the most important pathogens that contributes to BRD. In this study, we report the identification and first isolation, with molecular characterization, of a new BRSV strain from lung specimens of three beef cows in Turkey that died from respiratory distress. After the screening of lung tissues for BRD-associated viruses using a multiscreen antigen-ELISA, a BRSV antigen was detected. This was then confirmed by real-time RT-PCR specific for BRSV. Following confirmation, virus isolation was conducted in MDBK cell cultures and clear CPE, including syncytia compatible with BRSV, were detected. RT-nested PCR, using F gene-specific primers, was performed on the cultured isolates, and the products were sequenced and deposited to Genbank with accession numbers MT179304, MT024766, and MT0244767. Phylogenetic analysis of these sequences indicated that the cattle were infected with BRSV from subgroup III and were closely related to previously identified American and Turkish strains, but contained some amino acid and nucleotide differences. This research paves the way for further studies on the molecular characteristics of natural BRSV isolates, including full genome analysis and disease pathogenesis, and also contributes to the development of robust national strategies against this virus.

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“…BRSV infection in calves is widespread in America [20,21] , many European countries [1,8,[22][23][24] and Turkey according to serologic [10,11] and virologic [2] evidence. There is only one study on the investigation with virological methods of BRSV and also other some viruses causing the respiratory disease in calves.…”

Section: Discussionmentioning

confidence: 99%

Solunum Sistemi Hastalığı Olan Buzağılarda Saptanan Bovine Respiratory Syncytial Virusun Filogenetik Analizi

Karayel-Hacioglu¹,

Coşkun²,

Yelken³

et al. 2018

Kafkas Univ Vet Fak Derg

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Bovine respiratory disease (BRD) causes economic losses related to a reduction in weight gain of affected animals, veterinary treatment costs, death, etc. One of the important respiratory tract disease viruses is the bovine respiratory syncytial virus (BRSV). In this study, it is aimed to report the molecular characterization of detected BRSVs. Therefore, nasal samples from three calves in a herd seen severe respiratory disorders were examined for BRSV and other possible viral etiological agents by PCRs and the amplicons were sequenced. In the phylogenetic tree, BRSV circulating in this herd is clustered with the genetic subgroup III BRSVs deposited in GenBank from some other countries. This study on the molecular characterization of BRSV circulating in calves would contribute for future studies on the epidemiology of this infection and the development and/or choice the effective vaccines in Turkey.

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Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

Cited by 5 publications

References 17 publications

Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV

Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV

Circulation of Indigenous Bovine Respiratory Syncytial Virus Strains in Turkish Cattle: The First Isolation and Molecular Characterization

Solunum Sistemi Hastalığı Olan Buzağılarda Saptanan Bovine Respiratory Syncytial Virusun Filogenetik Analizi

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